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作物学报 ›› 2022, Vol. 48 ›› Issue (12): 3004-3017.doi: 10.3724/SP.J.1006.2022.13060

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

玉米转录因子ZmbHLH91对非生物逆境胁迫的应答

悦曼芳1,2(), 张春2(), 郑登俞2, 邹华文1(), 吴忠义2()   

  1. 1长江大学农学院, 湖北荆州 434025
    2北京市农林科学院生物技术研究所 / 农业基因资源与生物技术北京市重点实验室, 北京 100097
  • 收稿日期:2021-10-25 接受日期:2022-02-25 出版日期:2022-12-12 网络出版日期:2022-04-01
  • 通讯作者: 邹华文,吴忠义
  • 作者简介:悦曼芳, E-mail: 2278874241@qq.com;
    张春, E-mail: spring2007318@163.com第一联系人：

    **同等贡献

  • 基金资助:
    国家自然科学基金项目(32001430);国家自然科学基金项目(32171952);国家自然科学基金项目(31971839);北京市农林科学院科技创新能力建设专项(KJCX20200205);北京市农林科学院科技创新能力建设专项(KJCX20200407)

Response of maize transcriptional factor ZmbHLH91 to abiotic stress

YUE Man-Fang1,2(), ZHANG Chun2(), ZHENG Deng-Yu2, ZOU Hua-Wen1(), WU Zhong-Yi2()   

  1. 1College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China
    2Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences / Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing 100097, China
  • Received:2021-10-25 Accepted:2022-02-25 Published:2022-12-12 Published online:2022-04-01
  • Contact: ZOU Hua-Wen,WU Zhong-Yi
  • About author:First author contact:

    **Contributed equally to this work

  • Supported by:
    National Natural Science Foundation of China(32001430);National Natural Science Foundation of China(32171952);National Natural Science Foundation of China(31971839);Beijing Academy of Agriculture and Forestry Sciences(KJCX20200205);Beijing Academy of Agriculture and Forestry Sciences(KJCX20200407)

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摘要：

bHLH (basic helix-loop-helix)是植物中一个重要的转录因子家族, 在调控植物生长发育、逆境胁迫及信号转导过程中发挥着重要作用。目前, 动物中大部分bHLH转录因子功能已明确, 但是在植物中, 尤其是玉米中的研究报道较少。在前期工作中, 我们对玉米生长发育过程中的4个关键时期进行了根系表型鉴定和转录组测序分析, 发现转录因子ZmbHLH91在六叶期(V6)、十二叶期(V12)和抽雄期(VT)的相邻时期间的表达均差异显著, 且ZmbHLH91在V6, V12和VT这些根系生长发育活跃期的表达量较高, 推测该基因可能调控玉米根系的生长发育。为探究ZmbHLH91基因在根系生长和抵抗逆境胁迫方面的作用, 本研究克隆了ZmbHLH91 (AC: NC_AQL05369)基因, 该基因全长2112 bp, 具有bHLH转录因子家族特有的保守结构域。实时荧光定量PCR (RT-qPCR)分析表明, ZmbHLH91在玉米根中的表达量最高, 其在三叶期幼根中的表达量高于抽雄期成熟根。在高盐、渗透、低温以及脱水胁迫处理下, 玉米幼苗中ZmbHLH91的表达均上调。在无胁迫处理的1/2 MS培养基上, ZmbHLH91异源表达拟南芥与野生型拟南芥的根长无明显差别, 而在梯度浓度NaCl和甘露醇处理的培养基上, ZmbHLH91异源表达拟南芥的根均长于野生型, 且差异显著; 在土壤中进行干旱和高盐处理后, 转基因拟南芥比野生型拟南芥表现出更好的生长状态、更高的过氧化物酶(POD)活性和更高的绿叶率。推测ZmbHLH91基因可能参与响应高盐、干旱以及渗透胁迫等逆境条件。ZmbHLH91基因在茉莉酸(jasmonic acid, JA)、脱落酸(abscisic acid, ABA)等激素处理下均上调表达, 推测ZmbHLH91基因可能参与响应JA和ABA激素信号。在梯度浓度JA处理的培养基上, 转基因拟南芥的根长均长于野生型, 且差异显著。酵母双杂交实验表明ZmbHLH91与ZmMYC2相互作用, ZmMYC2是JA信号通路中重要的蛋白, 由此推测ZmbHLH91蛋白可能参与JA信号通路。综上所述, ZmbHLH91可能参与高盐、干旱和渗透胁迫应答及JA信号转导途径。本研究为进一步解析ZmbHLH91在玉米中的生物学功能提供了重要的参考依据。

关键词: 玉米, ZmbHLH91, 盐胁迫, 渗透胁迫, 酵母双杂, 信号转导

Abstract:

bHLH (basic helix-loop-helix) is an important transcription factor family in plants and play an important role in regulating plant growth and development, adversity stress, and signal transduction. At present, most bHLH transcription factor function in animals is clear, but there are few studies reported in plants, especially in maize. In the early work, we carried out root phenotype identification and transcriptome sequencing analysis in the four key periods of maize growth and development. There was significantly differentially expressed in ZmbHLH91 during the adjacent periods of the sixth leaf stage (V6), the twelfth leaf stage (V12), and the tasseling stage (VT). The relative expression levels of ZmbHLH91 were higher in V6, V12, and VT, which were the active periods of root growth and development. We speculated that ZmbHLH91 may have important regulatory effect on the growth and development of maize root system. To study the function of ZmbHLH91 in root growth and response to stress, in this study, we cloned ZmbHLH91 (AC:NC_AQL05369) gene. This gene was 2112 bp in full-length with a unique conserved domain of the bHLH transcription factor family. The RT-qPCR analysis showed that ZmbHLH91 had a higher expression in roots, whereas, the expression in the third leaf young root was higher than that in the tasseling mature root. While under different adversity stress, ZmbHLH91 was up-regulated. There was no significant difference in root length between ZmbHLH91 transgenic Arabidopsis strains and wild type on a 1/2 MS medium without stress treatment, but the root length of ZmbHLH91 transgenic Arabidopsis lines was longer than that of wild type on the medium treated with gradient concentrations NaCl and mannitol, and the difference was significant. Compared to the wild type, transgenic Arabidopsis lines revealed better growth status, higher peroxidase (POD) activity, higher green leaf rate after drought, and higher salt treatment in the soil. It is speculated that the ZmbHLH91 gene may be involved in responding to high salt, drought, and osmotic stress. The ZmbHLH91 was also up-regulated under jasmonic acid (JA), abscisic acid (ABA), and other hormone treatments. On the medium treated with gradient concentration JA, the root length of ZmbHLH91 transgenic Arabidopsis lines was longer than that of wild type, and the difference was significant. ZmbHLH91 interacted with the ZmMYC2 proved by yeast two hybrid system, speculating that it may be involved in the JA signaling pathway. In conclusion, ZmbHLH91 may be involved in high salt, drought, and osmosis stress response and JA signal transduction pathways. This study provides an important reference for further analysis of the biological functions of ZmbHLH91 in maize.

Key words: maize, ZmbHLH91, salt stress, osmotic stress, yeast two-hybrid, signal transduction

图2

ZmbHLH91表达量的分析 A: ZmbHLH91在玉米不同组织中的相对表达量; B: ZmbHLH91在V6、V12、VT、R3时期的表达量变化, C~F: ZmbHLH91分别在脱水、高盐、渗透、低温处理后的表达量; G: ZmbHLH91在玉米不同激素处理中的相对表达量。数据为3个生物学重复±标准差; 不同小写字母表示差异显著(P < 0.05); *: P < 0.05; **: P < 0.01。"

表1

本试验中所用的引物"

引物名称
Primer name		 引物序列
Primer sequence (5'-3')
pZmbHLH91-F TGAAATCACCAGTCGGTACCATGAACCTGTGGACGGACGA (Kpn I)
pZmbHLH91-R CCCTTGCTCACCATGGTACCCCTGCCCATGACAGACCCGG (Kpn I)
pZmbHLH91RT-F1 GTGGACATCAAGGATTCCTACT
pZmbHLH91RT-R1 GAAATCCGAGAAGTTGAGCTCC
pZmbHLH91RT-F2 TCCATGACGCAGTCCTTCCTCAACGG
pZmbHLH91RT-R2 ATGGTCTCGGCGGTCTGGAACACG
pZmbHLH91JM-F GGCCATGGAGGCCGAATTCATGAACCTGTGGACGGACGAC (EcoR I)
pZmbHLH91JM-R TATGCTAGTTATGCGGCCGCTTACCTGCCCATGACAGACC (BamH I)
pGAPDHRT-F CCCTTCATCACCACGGACTAC
pGAPDHRT-R AACCTTCTTGGCACCACCCT
pActinRT-F1 GGTAACATTGTGCTCAGTGGTGG
pActinRT-R1 AACGACCTTAATCTTCATGCTGC
pActinRT-F2 TACGAGATGCCTGATGGTCAGGTCA
pActinRT-R2 TGGAGTTGTACGTGGCCTCATGGAC

图1

ZmbHLH91生物信息学分析 A: 氨基酸序列比对; B: 跨膜结构预测; C: 蛋白空间结构预测; D: ZmbHLH91启动子序列分析。"

图3

T3代拟南芥PCR检测和RT-qPCR检测 A: T3代拟南芥PCR检测; B: T3代拟南芥RT-qPCR检测。M: DL5000 marker; P: 阳性对照; N: 阴性对照; W: 水对照; WT: 野生型拟南芥; L-1~L-6: T3代拟南芥; **: P < 0.01。"

图4

不同盐浓度下转基因拟南芥根长比较 A~D分别为 0、0.10、0.15和0.18 mol L-1盐处理的生长情况; E: 不同盐浓度下拟南芥平均主根长度。WT: 野生型拟南芥; L-1、L-2、L-3: 转ZmbHLH91拟南芥; 标尺为1.5 cm; *: P < 0.05; **: P < 0.01。"

图5

不同甘露醇浓度下转基因拟南芥根长比较 A~D分别为0、0.15、0.20和0.30 mol L-1甘露醇处理的生长情况; E: 不同甘露醇浓度下拟南芥平均主根长度。缩写同图4; 标尺为1.5 cm; *: P < 0.05; **: P < 0.01。"

图6

不同ABA和JA浓度下转基因拟南芥根长比较 A: 在0、10、25 和 50 µmol L-1 ABA处理下的生长情况; B: 不同ABA浓度下拟南芥平均主根长度; C: 0、50、75和100 µmol L-1 JA处理下的生长情况; D: 不同JA浓度下拟南芥平均主根长度。缩写同图4; 标尺为1.5 cm; *: P < 0.05; **: P < 0.01。"

图7

干旱处理下转基因拟南芥表型分析 A: 拟南芥表型; B: POD活性; C: 干旱处理后绿叶率; D: 复水处理后绿叶率。缩写同图4; **: P < 0.01。"

图8

高盐处理下转基因拟南芥表型分析 A: 拟南芥表型; B: POD活性; C: 高盐处理后绿叶率。缩写同图4; **: P < 0.01。"

图9

AD融合的玉米基因与ZmbHLH91互作 AD: pGADT7; BK: pGBKT7; SD2: SD-Leu-Trp缺陷型培养基; SD4: SD-Leu-Trp-His-Ade缺陷型培养基; P: 阳性对照(pGADT7-largeT+ pGBKT7-p53); N: 阴性对照(pGADT7-largeT+pGBKT7-laminC)。"

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