欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2022, Vol. 48 ›› Issue (12): 3004-3017.doi: 10.3724/SP.J.1006.2022.13060

所属专题： 玉米：遗传育种·种质资源·分子遗传学

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

玉米转录因子ZmbHLH91对非生物逆境胁迫的应答

悦曼芳1,2(), 张春2(), 郑登俞2, 邹华文1(), 吴忠义2()   

  1. 1长江大学农学院, 湖北荆州 434025
    2北京市农林科学院生物技术研究所 / 农业基因资源与生物技术北京市重点实验室, 北京 100097
  • 收稿日期:2021-10-25 接受日期:2022-02-25 出版日期:2022-12-12 网络出版日期:2022-04-01
  • 通讯作者: 邹华文,吴忠义
  • 作者简介:悦曼芳, E-mail: 2278874241@qq.com;
    张春, E-mail: spring2007318@163.com第一联系人：

    **同等贡献

  • 基金资助:
    国家自然科学基金项目(32001430);国家自然科学基金项目(32171952);国家自然科学基金项目(31971839);北京市农林科学院科技创新能力建设专项(KJCX20200205);北京市农林科学院科技创新能力建设专项(KJCX20200407)

Response of maize transcriptional factor ZmbHLH91 to abiotic stress

YUE Man-Fang1,2(), ZHANG Chun2(), ZHENG Deng-Yu2, ZOU Hua-Wen1(), WU Zhong-Yi2()   

  1. 1College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China
    2Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences / Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing 100097, China
  • Received:2021-10-25 Accepted:2022-02-25 Published:2022-12-12 Published online:2022-04-01
  • Contact: ZOU Hua-Wen,WU Zhong-Yi
  • About author:First author contact:

    **Contributed equally to this work

  • Supported by:
    National Natural Science Foundation of China(32001430);National Natural Science Foundation of China(32171952);National Natural Science Foundation of China(31971839);Beijing Academy of Agriculture and Forestry Sciences(KJCX20200205);Beijing Academy of Agriculture and Forestry Sciences(KJCX20200407)

RichHTML

43

PDF

406

摘要：

bHLH (basic helix-loop-helix)是植物中一个重要的转录因子家族, 在调控植物生长发育、逆境胁迫及信号转导过程中发挥着重要作用。目前, 动物中大部分bHLH转录因子功能已明确, 但是在植物中, 尤其是玉米中的研究报道较少。在前期工作中, 我们对玉米生长发育过程中的4个关键时期进行了根系表型鉴定和转录组测序分析, 发现转录因子ZmbHLH91在六叶期(V6)、十二叶期(V12)和抽雄期(VT)的相邻时期间的表达均差异显著, 且ZmbHLH91在V6, V12和VT这些根系生长发育活跃期的表达量较高, 推测该基因可能调控玉米根系的生长发育。为探究ZmbHLH91基因在根系生长和抵抗逆境胁迫方面的作用, 本研究克隆了ZmbHLH91 (AC: NC_AQL05369)基因, 该基因全长2112 bp, 具有bHLH转录因子家族特有的保守结构域。实时荧光定量PCR (RT-qPCR)分析表明, ZmbHLH91在玉米根中的表达量最高, 其在三叶期幼根中的表达量高于抽雄期成熟根。在高盐、渗透、低温以及脱水胁迫处理下, 玉米幼苗中ZmbHLH91的表达均上调。在无胁迫处理的1/2 MS培养基上, ZmbHLH91异源表达拟南芥与野生型拟南芥的根长无明显差别, 而在梯度浓度NaCl和甘露醇处理的培养基上, ZmbHLH91异源表达拟南芥的根均长于野生型, 且差异显著; 在土壤中进行干旱和高盐处理后, 转基因拟南芥比野生型拟南芥表现出更好的生长状态、更高的过氧化物酶(POD)活性和更高的绿叶率。推测ZmbHLH91基因可能参与响应高盐、干旱以及渗透胁迫等逆境条件。ZmbHLH91基因在茉莉酸(jasmonic acid, JA)、脱落酸(abscisic acid, ABA)等激素处理下均上调表达, 推测ZmbHLH91基因可能参与响应JA和ABA激素信号。在梯度浓度JA处理的培养基上, 转基因拟南芥的根长均长于野生型, 且差异显著。酵母双杂交实验表明ZmbHLH91与ZmMYC2相互作用, ZmMYC2是JA信号通路中重要的蛋白, 由此推测ZmbHLH91蛋白可能参与JA信号通路。综上所述, ZmbHLH91可能参与高盐、干旱和渗透胁迫应答及JA信号转导途径。本研究为进一步解析ZmbHLH91在玉米中的生物学功能提供了重要的参考依据。

关键词: 玉米, ZmbHLH91, 盐胁迫, 渗透胁迫, 酵母双杂, 信号转导

Abstract:

bHLH (basic helix-loop-helix) is an important transcription factor family in plants and play an important role in regulating plant growth and development, adversity stress, and signal transduction. At present, most bHLH transcription factor function in animals is clear, but there are few studies reported in plants, especially in maize. In the early work, we carried out root phenotype identification and transcriptome sequencing analysis in the four key periods of maize growth and development. There was significantly differentially expressed in ZmbHLH91 during the adjacent periods of the sixth leaf stage (V6), the twelfth leaf stage (V12), and the tasseling stage (VT). The relative expression levels of ZmbHLH91 were higher in V6, V12, and VT, which were the active periods of root growth and development. We speculated that ZmbHLH91 may have important regulatory effect on the growth and development of maize root system. To study the function of ZmbHLH91 in root growth and response to stress, in this study, we cloned ZmbHLH91 (AC:NC_AQL05369) gene. This gene was 2112 bp in full-length with a unique conserved domain of the bHLH transcription factor family. The RT-qPCR analysis showed that ZmbHLH91 had a higher expression in roots, whereas, the expression in the third leaf young root was higher than that in the tasseling mature root. While under different adversity stress, ZmbHLH91 was up-regulated. There was no significant difference in root length between ZmbHLH91 transgenic Arabidopsis strains and wild type on a 1/2 MS medium without stress treatment, but the root length of ZmbHLH91 transgenic Arabidopsis lines was longer than that of wild type on the medium treated with gradient concentrations NaCl and mannitol, and the difference was significant. Compared to the wild type, transgenic Arabidopsis lines revealed better growth status, higher peroxidase (POD) activity, higher green leaf rate after drought, and higher salt treatment in the soil. It is speculated that the ZmbHLH91 gene may be involved in responding to high salt, drought, and osmotic stress. The ZmbHLH91 was also up-regulated under jasmonic acid (JA), abscisic acid (ABA), and other hormone treatments. On the medium treated with gradient concentration JA, the root length of ZmbHLH91 transgenic Arabidopsis lines was longer than that of wild type, and the difference was significant. ZmbHLH91 interacted with the ZmMYC2 proved by yeast two hybrid system, speculating that it may be involved in the JA signaling pathway. In conclusion, ZmbHLH91 may be involved in high salt, drought, and osmosis stress response and JA signal transduction pathways. This study provides an important reference for further analysis of the biological functions of ZmbHLH91 in maize.

Key words: maize, ZmbHLH91, salt stress, osmotic stress, yeast two-hybrid, signal transduction

图2

ZmbHLH91表达量的分析 A: ZmbHLH91在玉米不同组织中的相对表达量; B: ZmbHLH91在V6、V12、VT、R3时期的表达量变化, C~F: ZmbHLH91分别在脱水、高盐、渗透、低温处理后的表达量; G: ZmbHLH91在玉米不同激素处理中的相对表达量。数据为3个生物学重复±标准差; 不同小写字母表示差异显著(P < 0.05); *: P < 0.05; **: P < 0.01。"

表1

本试验中所用的引物"

引物名称
Primer name		 引物序列
Primer sequence (5'-3')
pZmbHLH91-F TGAAATCACCAGTCGGTACCATGAACCTGTGGACGGACGA (Kpn I)
pZmbHLH91-R CCCTTGCTCACCATGGTACCCCTGCCCATGACAGACCCGG (Kpn I)
pZmbHLH91RT-F1 GTGGACATCAAGGATTCCTACT
pZmbHLH91RT-R1 GAAATCCGAGAAGTTGAGCTCC
pZmbHLH91RT-F2 TCCATGACGCAGTCCTTCCTCAACGG
pZmbHLH91RT-R2 ATGGTCTCGGCGGTCTGGAACACG
pZmbHLH91JM-F GGCCATGGAGGCCGAATTCATGAACCTGTGGACGGACGAC (EcoR I)
pZmbHLH91JM-R TATGCTAGTTATGCGGCCGCTTACCTGCCCATGACAGACC (BamH I)
pGAPDHRT-F CCCTTCATCACCACGGACTAC
pGAPDHRT-R AACCTTCTTGGCACCACCCT
pActinRT-F1 GGTAACATTGTGCTCAGTGGTGG
pActinRT-R1 AACGACCTTAATCTTCATGCTGC
pActinRT-F2 TACGAGATGCCTGATGGTCAGGTCA
pActinRT-R2 TGGAGTTGTACGTGGCCTCATGGAC

图1

ZmbHLH91生物信息学分析 A: 氨基酸序列比对; B: 跨膜结构预测; C: 蛋白空间结构预测; D: ZmbHLH91启动子序列分析。"

图3

T3代拟南芥PCR检测和RT-qPCR检测 A: T3代拟南芥PCR检测; B: T3代拟南芥RT-qPCR检测。M: DL5000 marker; P: 阳性对照; N: 阴性对照; W: 水对照; WT: 野生型拟南芥; L-1~L-6: T3代拟南芥; **: P < 0.01。"

图4

不同盐浓度下转基因拟南芥根长比较 A~D分别为 0、0.10、0.15和0.18 mol L-1盐处理的生长情况; E: 不同盐浓度下拟南芥平均主根长度。WT: 野生型拟南芥; L-1、L-2、L-3: 转ZmbHLH91拟南芥; 标尺为1.5 cm; *: P < 0.05; **: P < 0.01。"

图5

不同甘露醇浓度下转基因拟南芥根长比较 A~D分别为0、0.15、0.20和0.30 mol L-1甘露醇处理的生长情况; E: 不同甘露醇浓度下拟南芥平均主根长度。缩写同图4; 标尺为1.5 cm; *: P < 0.05; **: P < 0.01。"

图6

不同ABA和JA浓度下转基因拟南芥根长比较 A: 在0、10、25 和 50 µmol L-1 ABA处理下的生长情况; B: 不同ABA浓度下拟南芥平均主根长度; C: 0、50、75和100 µmol L-1 JA处理下的生长情况; D: 不同JA浓度下拟南芥平均主根长度。缩写同图4; 标尺为1.5 cm; *: P < 0.05; **: P < 0.01。"

图7

干旱处理下转基因拟南芥表型分析 A: 拟南芥表型; B: POD活性; C: 干旱处理后绿叶率; D: 复水处理后绿叶率。缩写同图4; **: P < 0.01。"

图8

高盐处理下转基因拟南芥表型分析 A: 拟南芥表型; B: POD活性; C: 高盐处理后绿叶率。缩写同图4; **: P < 0.01。"

图9

AD融合的玉米基因与ZmbHLH91互作 AD: pGADT7; BK: pGBKT7; SD2: SD-Leu-Trp缺陷型培养基; SD4: SD-Leu-Trp-His-Ade缺陷型培养基; P: 阳性对照(pGADT7-largeT+ pGBKT7-p53); N: 阴性对照(pGADT7-largeT+pGBKT7-laminC)。"

[1] Mao H, Yu L, Han R, Li Z, Liu H. ZmNAC55, a maize stress-responsive NAC transcription factor, confers drought resistance in transgenic Arabidopsis. Plant Physiol Biochem, 2016, 105: 55-66.
doi: 10.1016/j.plaphy.2016.04.018
[2] Li F, Liu W. Genome-wide identification, classification, and functional analysis of the basic helix-loop-helix transcription factors in the cattle, Bos Taurus. Mamm Genome, 2017, 28: 176-197.
doi: 10.1007/s00335-017-9683-x pmid: 28299435
[3] Murre C, McCaw P S, Vaessin H, Caudy M, Jan L Y, Jan Y N, Cabrera C V, Buskin J N, Hauschka S D, Lassar A B. Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence. Cell, 1989, 58: 537-544.
pmid: 2503252
[4] Jones S. An overview of the basic helix-loop-helix proteins. Genome Biol, 2004, 5: 226.
pmid: 15186484
[5] Murre C, McCaw P S, Baltimore D. A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins. Cell, 1989, 56: 777-783.
pmid: 2493990
[6] Wang C X, Qi C Y, Luo J H, Liu L, He Y, Chen L Q. Characterization of LRL5 as a key regulator of root hair growth in maize. Plant J, 2019, 98: 71-82.
doi: 10.1111/tpj.14200
[7] Jaqueth J S, Hou Z, Zheng P, Ren R, Nagel B A, Cutter G, Niu X, Vollbrecht E, Greene T W, Kumpatla S P. Fertility restoration of maize CMS-C altered by a single amino acid substitution within the Rf4 bHLH transcription factor. Plant J, 2020, 101: 101-111.
doi: 10.1111/tpj.14521
[8] Aslam M, Jakada B H, Fakher B, Greaves J G, Niu X, Su Z, Cheng Y, Cao S, Wang X, Qin Y. Genome-wide study of pineapple (Ananas comosus L.) bHLH transcription factors indicates that cryptochrome-interacting bHLH2 (AcCIB2) participates in flowering time regulation and abiotic stress response. BMC Genomics, 2020, 21: 735.
doi: 10.1186/s12864-020-07152-2
[9] Fan Y, Peng J, Wu J, Zhou P, He R, Allan A C, Zeng L. NtbHLH1, a JAF13-like bHLH, interacts with NtMYB6 to enhance proanthocyanidin accumulation in Chinese Narcissus. BMC Plant Biol, 2021, 21: 275.
doi: 10.1186/s12870-021-03050-1
[10] Guo J, Li W, Shang L, Wang Y, Yan P, Bai Y, Da X, Wang K, Guo Q, Jiang R, Mao C, Mo X. OsbHLH98 regulates leaf angle in rice through transcriptional repression of OsBUL1. New Phytol, 2021, 230: 1953-1966.
doi: 10.1111/nph.17303
[11] Nowak K, Gaj M D. Stress-related function of bHLH109 in somatic embryo induction in Arabidopsis. J Plant Physiol, 2016, 193: 119-126.
doi: 10.1016/j.jplph.2016.02.012
[12] Zhang L, Kang J, Xie Q, Gong J, Shen H, Chen Y, Chen G, Hu Z. The basic helix-loop-helix transcription factor bHLH95 affects fruit ripening and multiple metabolisms in tomato. J Exp Bot, 2020, 71: 6311-6327.
doi: 10.1093/jxb/eraa363
[13] Ortolan F, Fonini L S, Pastori T, Mariath J E A, Saibo N J M, Margis-Pinheiro M, Lazzarotto F. Tightly controlled expression of OsbHLH35 is critical for another development in rice. Plant Sci, 2021, 302: 110716.
[14] 汪德州, 莫小婷, 张霞, 徐妙云, 张兰, 赵军, 王磊. 玉米转录因子ZmbHLH4基因的克隆及功能分析. 中国农业科技导报, 2018, 20(12): 16-25.
doi: 10.13304/j.nykjdb.2018.0189
Wang D Z, Mo X T, Zhang X, Xu M Y, Zhang L, Zhao J, Wang L. Cloning and functional analysis of transcription factors gene ZmbHLH4 from Zea mays. J Agric Sci Technol, 2018, 20(12): 16-25. (in Chinese with English abstract)
[15] Yang Y Y, Zheng P F, Ren Y R, Yao Y X, You C X, Wang X F, Hao Y J. Apple MdSAT1 encodes a bHLHm1 transcription factor involved in salinity and drought responses. Planta, 2021, 253: 46.
doi: 10.1007/s00425-020-03528-6
[16] Liu Y, Ji X, Nie X, Qu M, Zheng L, Tan Z, Zhao H, Huo L, Liu S, Zhang B, Wang Y. Arabidopsis AtbHLH112 regulates the expression of genes involved in abiotic stress tolerance by binding to their E-box and GCG-box motifs. New Phytol, 2015, 207: 692-709.
doi: 10.1111/nph.13387
[17] Yang X, Wang R, Hu Q, Li S, Mao X, Jing H, Zhao J, Hu G, Fu J, Liu C. DlICE1, a stress-responsive gene from Dimocarpus longan, enhances cold tolerance in transgenic Arabidopsis. Plant Physiol Biochem, 2019, 142: 490-499.
doi: 10.1016/j.plaphy.2019.08.007
[18] Zhao Q, Fan Z, Qiu L, Che Q, Wang T, Li Y, Wang Y. MdbHLH130, an apple bHLH transcription factor, confers water stress resistance by regulating stomatal closure and ROS homeostasis in transgenic tobacco. Front Plant Sci, 2020, 11: 543696.
[19] Babitha K C, Vemanna R S, Nataraja K N, Udayakumar M. Overexpression of EcbHLH57 transcription factor from Eleusine coracana L. in tobacco confers tolerance to salt, oxidative and drought stress. PLoS One, 2015, 10: e137098.
[20] Qian Y, Zhang T, Yu Y, Gou L, Yang J, Xu J, Pi E. Regulatory mechanisms of bHLH transcription factors in plant adaptive responses to various abiotic stresses. Front Plant Sci, 2021, 12: 677611.
[21] Li Z, Liu C, Zhang Y, Wang B, Ran Q, Zhang J. The bHLH family member ZmPTF1 regulates drought tolerance in maize by promoting root development and abscisic acid synthesis. J Exp Bot, 2019, 70: 5471-5486.
doi: 10.1093/jxb/erz307
[22] Chen H C, Cheng W H, Hong C Y, Chang Y S, Chang M C. The transcription factor OsbHLH035 mediates seed germination and enables seedling recovery from salt stress through ABA- dependent and ABA-independent pathways, respectively. Rice (New York), 2018, 11: 1-17.
[23] Zhao Q, Xiang X, Liu D, Yang A, Wang Y. Tobacco transcription factor NtbHLH123 confers tolerance to cold stress by regulating the NtCBF pathway and reactive oxygen species homeostasis. Front Plant Sci, 2018, 9: 381.
doi: 10.3389/fpls.2018.00381
[24] Liu H, Yang Y, Liu D, Wang X, Zhang L. Transcription factor TabHLH49 positively regulates dehydrin WZY2gene expression and enhances drought stress tolerance in wheat. BMC Plant Biol, 2020, 20: 259.
doi: 10.1186/s12870-020-02474-5
[25] Wei S, Xia R, Chen C, Shang X, Ge F, Wei H, Chen H, Wu Y, Xie Q. ZmbHLH124 identified in maize recombinant inbred lines contributes to drought tolerance in crops. Plant Biotechnol J, 2021, 19: 2069-2081.
doi: 10.1111/pbi.13637
[26] 杨梦婷, 张春, 王作平, 邹华文, 吴忠义. 玉米ZmbHLH161基因的克隆及功能研究. 作物学报, 2020, 46: 2008-2016.
doi: 10.3724/SP.J.1006.2020.03022
Yang M T, Zhang C, Wang Z P, Zou H W, Wu Z Y. Cloning and functional analysis of ZmbHLH161 gene in maize. Acta Agron Sin, 2020, 46: 2008-2016. (in Chinese with English abstract)
[27] Rabissi A, Vilela B, Lumbreras V, Ludevid D, Culiáñez-Macià F A, Pagés M. Molecular characterization of maize bHLH transcription factor (ZmKS), a new ZmOST1 kinase substrate. Plant Sci, 2016, 253: 1-12.
doi: S0168-9452(16)30423-X pmid: 27968978
[28] Duek P D, Fankhauser C. HFR1, a putative bHLH transcription factor, mediates both phytochrome A and cryptochrome signalling. Plant J, 2003, 34: 827-836.
pmid: 12795702
[29] Kiribuchi K, Jikumaru Y, Kaku H, Minami E, Hasegawa M, Kodama O, Seto H, Okada K, Nojiri H, Yamane H. Involvement of the basic helix-loop-helix transcription factor RERJ1in wounding and drought stress responses in rice plants. Biosci Biotechnol Biochem, 2005, 69: 1042-1044.
doi: 10.1271/bbb.69.1042
[30] Castillon A, Shen H, Huq E. Phytochrome Interacting Factors: central players in phytochrome-mediated light signaling networks. Trends Plant Sci, 2007, 12: 514-521.
doi: 10.1016/j.tplants.2007.10.001 pmid: 17933576
[31] Friedrichsen D M, Nemhauser J, Muramitsu T, Maloof J N, Alonso J, Ecker J R, Furuya M, Chory J. Three redundant brassinosteroid early response genes encode putative bHLH transcription factors required for normal growth. Genetics, 2002, 162: 1445-1456.
doi: 10.1093/genetics/162.3.1445 pmid: 12454087
[32] Seo J S, Joo J, Kim M J, Kim Y K, Nahm B H, Song S I, Cheong J J, Lee J S, Kim J K, Choi Y D. OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice. Plant J, 2011, 65: 907-921.
doi: 10.1111/j.1365-313X.2010.04477.x
[33] Zhang T, Lv W, Zhang H, Ma L, Li P, Ge L, Li G. Genome-wide analysis of the basic Helix-Loop-Helix (bHLH) transcription factor family in maize. BMC Plant Biol, 2018, 18: 235.
doi: 10.1186/s12870-018-1441-z pmid: 30326829
[34] Zhang C, Li X, Wang Z, Zhang Z, Wu Z. Identifying key regulatory genes of maize root growth and development by RNA sequencing. Genomics, 2020, 112: 5157-5169.
doi: 10.1016/j.ygeno.2020.09.030 pmid: 32961281
[35] Clough S J, Bent A F. Floral dip: a simplified method for Agrobacterium mediated transformation of Arabidopsis thaliana. Plant J, 1998, 16: 735-743.
pmid: 10069079
[36] Chini A, Fonseca S, Fernández G, Adie B, Chico J M, Lorenzo O, García-Casado G, López-Vidriero I, Lozano F M, Ponce M R, Micol J L, Solano R. The JAZ family of repressors is the missing link in jasmonate signalling. Nature, 2007, 448: 666-671.
doi: 10.1038/nature06006
[37] Fernández-Calvo P, Chini A, Fernández-Barbero G, Chico J M, Gimenez-Ibanez S, Geerinck J, Eeckhout D, Schweizer F, Godoy M, Franco-Zorrilla J M, Pauwels L, Witters E, Puga M I, Paz-Ares J, Goossens A, Reymond P, De Jaeger G, Solano R. The Arabidopsis bHLH transcription factors MYC3 and MYC4 are targets of JAZ repressors and act additively with MYC2 in the activation of jasmonate responses. Plant Cell, 2011, 23: 701-715.
doi: 10.1105/tpc.110.080788
[38] Liu X, Galli M, Camehl I, Gallavotti A. RAMOSA1 ENHANCER LOCUS2-mediated transcriptional repression regulates vegetative and reproductive architecture. Plant Physiol, 2019, 179: 348-363.
doi: 10.1104/pp.18.00913 pmid: 30348817
[39] Fu J, Liu L, Liu Q, Shen Q, Wang C, Yang P, Zhu C, Wang Q. ZmMYC2 exhibits diverse functions and enhances JA signaling in transgenic Arabidopsis. Plant Cell Rep, 2020, 39: 273-288.
doi: 10.1007/s00299-019-02490-2
[1] 刘恩波, 陈静, 李红星, 于宁宁, 任佰朝, 赵斌, 刘鹏, 张吉旺. 遮阴改变源-库平衡和调节碳水化合物代谢进而抑制夏玉米幼穗发育[J]. 作物学报, 2026, 52(6): 1891-1901.
[2] 左同鸿, 张贺翠, 曾静, 朱利泉. 甘蓝自交不亲和相关基因BoPUB3L的克隆与表达分析[J]. 作物学报, 2026, 52(6): 1698-1710.
[3] 梁进宇, 尹嘉德, 王红丽, 张国平, 侯慧芝, 董博, 马明生. 基于无人机高光谱和机器学习的旱地饲用玉米叶片氮含量估测[J]. 作物学报, 2026, 52(6): 1788-1801.
[4] 邹仪妹, 徐敏, 汪海洋, 姚辉, 王加峰, 刘浩, 任代胜. 两系不育系水稻幼苗根系响应盐胁迫的转录因子调控网络鉴定[J]. 作物学报, 2026, 52(6): 1728-1742.
[5] 杨欣雨, 崔文涛, 迪力尼格尔·阿力木, 汪凯翔, 吴鹏昊, 任姣姣. 玉米穗上叶片数全基因组关联分析和全基因组选择[J]. 作物学报, 2026, 52(5): 1573-1590.
[6] 韩亚鑫, 何冠华, 张小琼, 张登峰, 李永祥, 刘旭洋, 王天宇, 黎裕, 邹华文, 李春辉. 基于RNA-Seq和BSA-Seq联合分析挖掘玉米侧根密度基因资源[J]. 作物学报, 2026, 52(5): 1341-1352.
[7] 孙淑凤, 许振南, 黄嘉鑫, 翁建峰, 李新海. 玉米MAPK家族全基因组鉴定及其对拟轮枝镰孢菌感染的响应[J]. 作物学报, 2026, 52(5): 1291-1308.
[8] 张宁宁, 滕雨菲, 任娜娜, 魏兴卓, 闫书豪, 樊可心, 王永宏, 陈文康, 张兴华, 朱万超, 徐淑兔, 薛吉全. 201份玉米自交系抗旱表型评价及可塑性分析[J]. 作物学报, 2026, 52(5): 1309-1325.
[9] 杨扬, 常诗惠, 田红丽, 易红梅, 王璐, 任洁, 范亚明, 刘亚维, 王凤格, 赵久然. 不同生态区国审玉米品种的遗传多样性分析[J]. 作物学报, 2026, 52(5): 1352-1364.
[10] 张鸿蓉, 王菲儿, 李盼, 仇海龙, 朱静, 赵连豪, 南运有, 何蔚, 樊志龙, 胡发龙, 柴强, 殷文. 减量20%灌水与25%有机肥替代化肥提高青贮玉米产量的光合特性[J]. 作物学报, 2026, 52(5): 1487-1500.
[11] 蔡宏玮, 于爱忠, 姜科强, 王鹏飞, 王玉珑, 霍建喆, 庞小能, 尹波, 尚永盼. 干旱灌区有机肥替代部分化肥促进甜玉米产量提升的关键机制[J]. 作物学报, 2026, 52(4): 1166-1180.
[12] 田红丽, 杨扬, 范亚明, 易红梅, 郭丹丹, 王凤格, 赵久然. 适于玉米品种鉴定的一套三等位变异SNP新型标记组合[J]. 作物学报, 2026, 52(4): 993-1005.
[13] 杨亚莉, 徐明睿, 马越飞, 海艺蕊, 刘凯栋, 刘万茂, 孙颖. 玉米根尖及整根响应缺铁的转录组比较研究[J]. 作物学报, 2026, 52(4): 1006-1021.
[14] 宋裕祯, Bheel Chander Kumar, 王跃, 张颖星, 郭娟, Khound Rituraj, Santra Dipak Kumar, 曹晓宁, 王瑞云. 糜子AP2亚家族全基因组鉴定及PmAP2-1和PmAP2-9耐盐功能分析[J]. 作物学报, 2026, 52(4): 1127-1139.
[15] 张超, 郭欢, 李忠玲, 岳淑宁, 赵娜. 基于BSA-seq技术定位玉米籽粒花青素关联基因[J]. 作物学报, 2026, 52(3): 780-789.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2