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作物学报 ›› 2023, Vol. 49 ›› Issue (4): 906-916.doi: 10.3724/SP.J.1006.2023.21029

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦转录因子TaMYB5-3B与株高和千粒重相关

朱治1,2(), 李龙2, 李超男2, 毛新国2, 郝晨阳2, 朱婷2, 王景一2,*(), 常建忠1,*(), 景蕊莲2   

  1. 1山西农业大学山西有机旱作农业研究院/有机旱作山西省重点实验室/省部共建有机旱作国家重点实验室(筹), 山西太原 030031
    2中国农业科学院作物科学研究所, 北京 100081
  • 收稿日期:2022-04-21 接受日期:2022-07-21 出版日期:2023-04-12 网络出版日期:2022-08-29
  • 通讯作者: *常建忠, E-mail: cjzyfx@163.com;王景一, E-mail: wangjingyi@caas.cn
  • 作者简介:E-mail: zz1752782610@163.com
  • 基金资助:
    山西农业大学省部共建有机旱作农业国家重点实验室自主研发项目(202105D121008-2-7);财政部和农业农村部国家现代农业产业技术体系建设专项(小麦, CARS-03)

Transcription factor TaMYB5-3B is associated with plant height and 1000- grain weight in wheat

ZHU Zhi1,2(), LI Long2, LI Chao-Nan2, MAO Xin-Guo2, HAO Chen-Yang2, ZHU Ting2, WANG Jing-Yi2,*(), CHANG Jian-Zhong1,*(), JING Rui-Lian2   

  1. 1Shanxi Institute of Organic Dryland Farming, Shanxi Key Laboratory of Organic Dry Farming, State Key Laboratory of Integrative Sustainable Dryland Agriculture (in Preparation), Shanxi Agricultural University, Taiyuan 030031, Shanxi, China
    2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2022-04-21 Accepted:2022-07-21 Published:2023-04-12 Published online:2022-08-29
  • Contact: *E-mail: cjzyfx@163.com;E-mail: wangjingyi@caas.cn
  • Supported by:
    State Key Laboratory of Integrative Sustainable Dryland Agriculture, the Shanxi Agricultural University(202105D121008-2-7);China Agriculture Research System of MOF and MARA(Wheat, CARS-03)

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摘要：

MYB转录因子在植物生长发育过程中发挥着重要作用。本研究克隆了小麦3B染色体上的TaMYB5-3B基因, 基因组序列全长3005 bp, 其中编码区上游为2112 bp, 编码区为893 bp, 包含2个外显子和1个内含子, 编码一个R2R3-MYB蛋白。序列多态性分析表明, 在TaMYB5-3B的-2048、-1632、-1178、-1156、-504、-461、-433和61 bp处各有1个SNP位点, 分别是G/A转换、G/A转换、G/A转换、T/C转换、C/T转换、A缺失、T缺失和T/A颠换, 这8个SNP位点连锁。基于启动子区SNP-1632的变异开发分子标记, 检测小麦自然群体的基因型, 与表型性状进行关联分析, 结果显示TaMYB5-3B与株高、穗下节长和千粒重显著相关。TaMYB5-3B在群体中有2种单倍型Hap-3B-1和Hap-3B-2, 其中Hap-3B-2是植株较矮、千粒重较高的优异单倍型。在我国的小麦育种历程中Hap-3B-2受到了正向选择, 在育成品种中的频率逐步增加, 但仍然有进一步的应用潜力。研究结果为深入探讨小麦株高和产量的形成机制提供参考, 也为小麦株型和产量分子育种提供了基因资源与选择标记。

关键词: 分子标记, 株高, 千粒重, 单倍型, 关联分析, 小麦

Abstract:

MYB transcription factor plays an important role in plant growth and development. In this study, we cloned TaMYB5-3B gene on chromosome 3B in wheat. The full-length genome sequence is 3005 bp, including 2112 bp promoter region and 893 bp coding region. TaMYB5-3B coding region consists of two exons and one intron, which encodes a R2R3-MYB protein. The polymorphism of TaMYB5-3B was analyzed by sequencing 32 wheat accessions with wide variations. A total of eight SNPs were detected at -2048, -1632, -1178, -1156, -504, -461, -433, and 61 bp, respectively. They were eight SNPs linked by G/A conversion, G/A conversion, G/A conversion, T/C conversion, C/T conversion, A deletion, T deletion and T/A inversion, respectively. A pair of molecular markers were developed based on the promoter region SNP-1632 to detect the genotypes of wheat natural population. The association analysis of genotype and phenotypic traits showed that TaMYB5-3B was significantly associated with plant height (PH), peduncle length (PLE), and 1000-grain weight (TGW). Two haplotypes (Hap-3B-1 and Hap-3B-2) were detected in the population, in which Hap-3B-2 was an excellent haplotype with short PH and high TGW. Hap-3B-2 had been positively selected in the breeding, and its frequency in modern cultivars gradually increased with the advance of breeding years in China. Therefore, TaMYB5-3B could be used to further understand the mechanism of wheat plant height and grain yield formation, and its molecular markers may contribute to ideal plant architecture and grain yield breeding of wheat.

Key words: molecular markers, plant height, 1000-grain weight, haplotype, association analysis, wheat

表1

32份高多态性小麦材料的信息"

序号
Number		 材料
Accession name		 来源
Origin
1 安85中124-1 An 85 Zhong 124-1 中国北京Beijing, China
2 北京10号 Beijing 10 中国北京Beijing, China
3 北京14号 Beijing 14 中国北京Beijing, China
4 北京8686 Beijing 8686 中国北京Beijing, China
5 单R8093 Dan R8093 中国北京Beijing, China
6 丰抗13 Fengkang 13 中国北京Beijing, China
7 京411 Jing 411 中国北京Beijing, China
8 京核8922 Jinghe 8922 中国北京Beijing, China
9 京品10号 Jingpin 10 中国北京Beijing, China
10 04-030 中国北京Beijing, China
11 04-044 中国北京Beijing, China
12 晋2148-7 Jin 2148-7 中国福建Fujian, China
13 白齐麦 Baiqimai 中国甘肃Gansu, China
14 霸王鞭 Bawangbian 中国河北Hebei, China
15 沧州小麦 Cangzhouxiaomai 中国河北Hebei, China
16 冀麦6号 Jimai 6 中国河北Hebei, China
17 冀麦41 Jimai 41 中国河北Hebei, China
18 白糙麦 Baicaomai 中国河南Henan, China
19 内乡188 Neixiang 188 中国河南Henan, China
20 偃展1号 Yanzhan 1 中国河南Henan, China
21 紫杆白芒先 Ziganbaimangxian 中国河南Henan, China
22 昌乐5号 Changle 5 中国山东Shandong, China
23 长6878 Chang 6878 中国山西Shanxi, China
24 红和尚 Hongheshang 中国山西Shanxi, China
25 临抗5108 Linkang 5108 中国山西Shanxi, China
26 长武131 Changwu 131 中国陕西Shaanxi, China
27 大荔1号 Dali 1 中国陕西Shaanxi, China
28 中国春 Chinese Spring 中国四川Sichuan, China
29 9th-5-1 国际玉米小麦改良中心CIMMYT
30 9th-25 国际玉米小麦改良中心CIMMYT
31 9th-50-1 国际玉米小麦改良中心CIMMYT
32 PANDAS 意大利Italy

表2

本研究所用引物"

引物名称
Primer name		 引物序列
Primer sequence (5'-3')		 试验目的
Experimental purpose
TaMYB5-3B-F1 GCCAGATCCGTCAAGCAATTCATGT 克隆基因编码区 Cloning gene coding region
TaMYB5-3B-R1 GCAACGTTTCCCAACATGTGTGC 克隆基因编码区 Cloning gene coding region
TaMYB5-3B-F2 GCAAACGAGGGCTGAATACCAATCA 克隆基因启动子区 Cloning gene coding region
TaMYB5-3B-R2 ATGTTGCCGGAGCTCATCCACTA 克隆基因启动子区/测序 Cloning gene coding region
TaMYB5-3B-dCAPS-F ACCAACTACTTTGGGGGTGCAGA dCAPS分子标记 dCAPS molecular markers
TaMYB5-3B-dCAPS-R GCAACGTTTCCCAACATGTGTGC dCAPS分子标记 dCAPS molecular markers
TaMYB5-3B-cDNA-F GCCAGATCCGTCAAGCAATTCATGT 克隆cDNA/测序 Cloning cDNA/sequencing
TaMYB5-3B-cDNA-R TCTCAGTCTCAGAAAAAGCGTCCGA 克隆cDNA/测序 Cloning cDNA/sequencing
TaMYB-3B-seq-R CAACATGTGGCGTGAGTTCCTCTC 测序 Sequencing
TaMYB-3B-seq-F GAGAGGAACTCACGCCACATGTTG 测序 Sequencing

图1

TaMYB5蛋白序列比对及亲缘关系 A: TaMYB5氨基酸序列比对图。黑线指示R2和R3结构域; B: TaMYB5蛋白进化树。TaMYB5-3B用红点标注, Ta: 小麦; Td: 野生二粒小麦; Hv: 大麦; Bd: 二穗短柄草; Sb: 高粱; Zm: 玉米; Si: 谷子; Ph: 哈氏黍; Pv: 柳枝稷; Ob: 短花药野生稻; Os: 水稻。"

图2

TaMYB5-3B的核苷酸多态性和分子标记开发 A: TaMYB5-3B的结构示意图与多态性位点, 红色字母表示设计分子标记的SNP位点; B: 分子标记dCAPS-1632的开发, 红框和红点代表通过碱基T错配为C引入Bgl II酶切位点, 红色字母代表2种单倍型碱基差异; C: PCR产物用Bgl II酶切的结果。M: 100 bp DNA ladder。"

表3

小麦dCAPS-1632标记与农艺性状关联分析"

年份
Year		 地点
Site		 处理
Treatment		 性状Trait
株高PH 穗下节长PLE 千粒重TGW
2015 顺义Shunyi WW 0.01404* 0.01882* ns
WW+HS 0.00191** 0.01511* 0.01324*
DS ns ns 0.00139**
DS+HS 0.04595* 0.02753* 0.01404*
2016 顺义Shunyi WW 0.01204* 0.01945* ns
WW+HS 0.00600** 0.02717* ns
DS 0.00661** 0.00912** 0.01480*
DS+HS 0.01621* 0.00883** ns
昌平Changping WW 0.04117* ns 0.04570*
DS 0.03954* ns 0.00178**
2017 顺义Shunyi WW 0.00621** 0.01578* 9.93E-05***
WW+HS 0.00860** 0.03151* 0.00514**
DS 0.02229* ns 0.00405**
DS+HS 0.00712** 0.00773** ns
昌平Changping WW 0.02190* 0.01346* 0.00625**
DS 0.02983* 0.02417* 0.01491*

图3

TaMYB5-3B两种单倍型的农艺性状对比 A~C: TaMYB5-3B的2种单倍型在16种环境中株高(A)、穗下节长(B)和千粒重(C)的比较; D: 2种单倍型在3种环境中株高和千粒重的比较。E: 环境, E1: 15-SY-WW; E2: 15-SY-WW-HS; E3: 15-SY-DS; E4: 15-SY-DS-HS; E5: 16-SY-WW; E6: 16-SY-WW-DS; E7: 16-SY-DS; E8: 16-SY-DS-HS; E9: 16-CP-WW; E10: 16-CP-DS; E11: 17-SY-WW; E12: 17-SY-WW-HS; E13: 17-SY-DS; E14: 17-SY-DS-HS; E15: 17-CP-WW; E16: 17-CP-DS。15: 2015; 16: 2016; 17: 2017; 02: 2002; 05: 2005; 10: 2010。SY: 顺义; CP: 昌平; LY: 洛阳。WW: 水分充足; DS: 干旱胁迫; HS: 高温胁迫。数据显著性采用t检验。**P < 0.01; ***P < 0.001。误差值: ±SE。"

图4

TaMYB5-3B两种单倍型的频率与分布 A~B: TaMYB5-3B两种单倍型在中国10个小麦产区的157个地方品种(A)和348个现代育成品种(B)中的分布。I: 北方冬麦区; II: 黄淮冬麦区; III: 长江中下游麦区; IV: 西南冬麦区; V: 华南冬麦区; VI: 东北春麦区; VII: 北部春麦区; VIII: 西北春麦区; IX: 青藏春冬麦区; X: 新疆冬春麦区。"

图5

我国348个育成品种中TaMYB5-3B单倍型频率以及PH和TGW变化 A: 随年代推进两个单倍型Hap-3B-1和Hap-3B-2在群体3中的频率变化; B: 随年代推进PH和TGW在群体3中的变化。误差值: ±SE。PH和TGW的数据来自Hao等[23]。"

[1] Ray D K, Mueller N D, West P C, Foley J A, Hart J P. Yield trends are insufficient to double global crop production by 2050. PLoS One, 2013, 8: e66428.
doi: 10.1371/journal.pone.0066428
[2] Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L. MYB transcription factors in Arabidopsis. Trends Plant Sci, 2010, 15: 573-581.
doi: 10.1016/j.tplants.2010.06.005
[3] Paz-Ares J, Ghosal D, Wienand U, Peterson P A, Saedler H. The regulatory cl locus of Zea mays encodes a protein with homology to MYB-related proto-oncogene products and with structural similarities to transcriptional activators. EMBO J, 1987, 6: 3553-3558.
doi: 10.1002/j.1460-2075.1987.tb02684.x pmid: 3428265
[4] Li J L, Han G L, Sun C F, Sui N. Research advances of MYB transcription factors in plant stress resistance and breeding. Plant Signal Behav, 2019, 14: 1-9.
[5] 陈清, 汤浩茹, 董晓莉, 侯艳霞, 罗娅, 蒋艳, 黄琼瑶. 植物MYB转录因子的研究进展. 基因组学与应用生物学, 2009, 28: 365-372.
Chen Q, Tang H R, Dong X L, Hou Y X, Luo Y, Jiang Y, Huang Q Y. Progress in the study of plant MYB transcription factors. Genom Appl Biol, 2009, 28: 365-372. (in Chinese with English abstract)
[6] Yang X, Li X, Shan J M, Li Y H, Zhang Y T, Wang Y H, Li W B, Zhao L. Overexpression of GmGAMYB accelerates the transition to flowering and increases plant height in soybean. Front Plant Sci, 2021, 12: 667242.
doi: 10.3389/fpls.2021.667242
[7] Mu R L, Cao Y R, Liu Y F, Lei G, Zou H F, Liao Y, Wang H W, Zhang W K, Ma B, Du J Z. An R2R3-type transcription factor gene AtMYB59 regulates root growth and cell cycle progression in Arabidopsis. Cell Res, 2009, 19: 1291-1304.
doi: 10.1038/cr.2009.83
[8] Yang Y, Zhang L B, Chen P, Liang T, Li X, Liu H T. UV-B photoreceptor UVR8 interacts with MYB73/MYB77 to regulate auxin responses and lateral root development. EMBO J, 2020, 39: e101928.
[9] Seo P J, Xiang F, Qiao M, Park J Y, Park C M. The MYB96 transcription factor mediates abscisic acid signaling during drought stress response in Arabidopsis. Plant Physiol, 2009, 151: 275-289.
doi: 10.1104/pp.109.144220
[10] Li Y F, Zeng X Q, Li Y, Wang L, Zhuang H, Wang Y, Tang J, Wang H L, Xiong M, Yang F Y. MULTI-FLORET SPIKELET 2, a MYB transcription factor, determines spikelet meristem fate and floral organ identity in rice. Plant Physiol, 2020, 184: 988-1003.
doi: 10.1104/pp.20.00743
[11] Yamagishi M, Shimoyamada Y, Nakatsuka T, Masuda K. Two R2R3-MYB genes, homologs of petunia AN2, regulate anthocyanin bio-syntheses in flower tepals, tepal spots and leaves of Asiatic hybrid lily. Plant Cell Physiol, 2010, 51: 463-474.
doi: 10.1093/pcp/pcq011 pmid: 20118109
[12] Li G L, Xu B X, Zhang Y P, Xu Y W, Khan N U, Xie J Y, Sun X M, Guo H F, Wu Z Y, Wang X Q, Zhang H L, Li J J, Xu J L, Wang W S, Zhang Z Y, Li Z C. RGN1 controls grain number and shapes panicle architecture in rice. Plant Biotechnol J, 2021, 20: 158-167.
doi: 10.1111/pbi.13702
[13] Ren D Y, Cui Y J, Hu H T, Xu Q K, Rao Y C, Yu X Q, Zhang Y, Wang Y X, Peng Y L, Zeng D L, Hu J, Zhang G H, Gao Z Y, Zhu L, Chen G, Shen L, Zhang Q, Guo L B, Qian Q. AH2 encodes a MYB domain protein that determines hull fate and affects grain yield and quality in rice. Plant J, 2019, 100: 813-824.
doi: 10.1111/tpj.14481
[14] Zhang Y X, Yu C S, Lin J Z, Liu J, Liu B, Wang J, Huang A, Li H Y, Zha T. OsMPH1 regulates plant height and improves grain yield in rice. PLoS One, 2017, 12: e0180825.
doi: 10.1371/journal.pone.0180825
[15] Azuma A, Udo Y, Sato A, Mitani N, Kono A, Ban Y, Yakushiji H, Koshita Y, Kobayashi S. Haplotype composition at the color locus is a major genetic determinant of skin color variation in Vitis × labruscana grapes. Theor Appl Genet, 2011, 122: 1427-1438.
doi: 10.1007/s00122-011-1542-7
[16] Jiu S T, Guan L, Leng X P, Zhang K K, Haider M S, Yu X, Zhu X D, Zheng T, Ge M Q, Wang C, Jia H F, Shang-Guan L F, Zhang C X, Tang X P, Abdullah M, Javed H U, Han J, Dong Z G, Fang J G. The role of VvMYBA2r and VvMYBA2w alleles of the MYBA2 locus in the regulation of anthocyanin biosynthesis for molecular breeding of grape (Vitis spp.) skin coloration. Plant Biotechnol J, 2021, 19: 1216-1239.
doi: 10.1111/pbi.13543
[17] Zheng X W, Liu C, Qiao L, Zhao J J, Han R, Wang X L, Ge C, Zhang W Y, Zhang S W, Qiao L Y, Zheng J, Hao C Y. The MYB transcription factor TaPHR3-A1 is involved in phosphate signaling and governs yield-related traits in bread wheat. J Exp Bot, 2020, 71: 5808-5822.
doi: 10.1093/jxb/eraa355 pmid: 32725154
[18] Liu Y N, He Z H, Appels R, Xia X C. Functional markers in wheat: current status and future prospects. Theor Appl Genet, 2012, 125: 1-10.
doi: 10.1007/s00122-012-1829-3 pmid: 22366867
[19] Kage U, Kumar A, Dhokane D, Karre S, Kushalappa A C. Functional molecular markers for crop improvement. Crit Rev Biotechnol, 2016, 36: 917-930.
doi: 10.3109/07388551.2015.1062743 pmid: 26171816
[20] Salgotra R K, Stewart C N Jr. Functional markers for precision plant breeding. Int J Mol Sci, 2020, 21: 4792.
doi: 10.3390/ijms21134792
[21] 张宏娟, 李玉莹, 苗丽丽, 王景一, 李超男, 杨德龙, 毛新国, 景蕊莲. 小麦转录因子基因TaNAC67参与调控穗长和每穗小穗数. 作物学报, 2019, 45: 1615-1627.
doi: 10.3724/SP.J.1006.2019.91009
Zhang H J, Li Y Y, Miao L L, Wang J Y, Li C N, Yang D L, Mao X G, Jing R L. Transcription factor gene TaNAC67 involved in regulation spike length and spikelet number per spike in common wheat. Acta Agron Sin, 2019, 45: 1615-1627. (in Chinese with English abstract)
[22] Li L, Mao X G, Wang J Y, Chang X P, Reynolds M, Jing R L. Genetic dissection of drought and heat-responsive agronomic traits in wheat. Plant Cell Environ, 2019, 42: 2540-2553.
doi: 10.1111/pce.13577
[23] Hao C Y, Wang L F, Ge H M, Dong Y C, Zhang X Y. Genetic diversity and linkage disequilibrium in Chinese bread wheat (Triticum aestivum L.) revealed by SSR markers. PLoS One, 2011, 6: e17279.
doi: 10.1371/journal.pone.0017279
[24] Cao S H, Xu D G, Hanif M, Xia X C, He Z H. Genetic architecture underpinning yield component traits in wheat. Theor Appl Genet, 2020, 133: 1811-1823.
doi: 10.1007/s00122-020-03562-8 pmid: 32062676
[25] Khobra R, Sareen S, Meena B K, Kumar A, Tiwari V, Singh G P. Exploring the traits for lodging tolerance in wheat genotypes: a review. Physiol Mol Biol Plants, 2019, 25: 589-600.
doi: 10.1007/s12298-018-0629-x
[26] Foulkes M, Slafer G, Davies W, Berry P, Sylvester-Bradley R, Martre P, Calderini D F, Griffiths S, Reynolds M P. Raising yield potential of wheat: III. Optimizing partitioning to grain while maintaining lodging resistance. J Exp Bot, 2011, 62: 469-486.
doi: 10.1093/jxb/erq300 pmid: 20952627
[27] Yang X Y, Li J G, Pei M, Gu H, Chen Z L, Qu L J. Over-expression of a flower-specific transcription factor gene AtMYB24 causes aberrant anther development. Plant Cell Rep, 2007, 26: 219-228.
pmid: 16972096
[28] 王诺菡, 于霁雯, 吴嫚, 马启峰, 李兴丽, 裴文锋, 李海晶, 黄双领, 张金发, 喻树迅. 棉花GhMYB0基因的克隆、表达分析及功能鉴定. 作物学报, 2014, 40: 1540-1548.
doi: 10.3724/SP.J.1006.2014.01540
Wang N H, Yu J W, Wu M, Ma Q F, Li X L, Pei W F, Li H J, Huang S L, Zhang J F, Yu S X. Cloning, expression, and functional analysis of GhMYB0 gene from cotton (Gossypium hirsumtum L.). Acta Agron Sin, 2014, 40: 1540-1548. (in Chinese with English abstract)
doi: 10.3724/SP.J.1006.2014.01540
[29] Yang H, Xue Q, Zhang Z Z, Du J Y, Yu D Y, Fang H. GmMYB181, a soybean R2R3-MYB protein, increases branch number in transgenic Arabidopsis. Front Plant Sci, 2018, 9: 1027.
doi: 10.3389/fpls.2018.01027 pmid: 30065741
[30] Cheng Q, Dong L D, Su T, Li T Y, Gan Z R, Nan H Y, Lu S J, Fang C, Kong L P, Li H Y, Hou Z H, Kou K, Tang Y, Lin X Y, Zhao X H, Chen L Y, Liu B H, Kong F J. CRISPR/Cas9-mediated targeted mutagenesis of GmLHY genes alters plant height and internode length in soybean. BMC Plant Biol, 2019, 19: 562.
doi: 10.1186/s12870-019-2145-8 pmid: 31852439
[31] Mao H D, Jian C, Cheng X X, Chen B, Mei F M, Li F F, Zhang Y F, Li S M, Du L Y, Li T, Hao C Y, Wang X J, Zhang X Y, Kang Z S. The wheat ABA receptor gene TaPYL1-1B contributes to drought tolerance and grain yield by increasing water-use efficiency. Plant Biotechnol J, 2022, 20: 846-861.
doi: 10.1111/pbi.13764
[32] Carter A H, Garland-Campbell K, Kidwell K K. Genetic mapping of quantitative trait loci associated with important agronomic traits in the spring wheat (Triticum aestivum L.) cross ‘Louise’ × ‘Penawawa’. Crop Sci, 2011, 51: 84-95.
doi: 10.2135/cropsci2010.03.0185
[33] Liu J, Wu B H, Singh R P, Velu G. QTL mapping for micronutrients concentration and yield component traits in a hexaploid wheat mapping population. J Cereal Sci, 2019, 88: 57-64.
doi: 10.1016/j.jcs.2019.05.008
[34] Cui F, Zhao C H, Ding A M, Li J, Wang L, Li X F, Bao Y G, Li J M, Wang H G. Construction of an integrative linkage map and QTL mapping of grain yield-related traits using three related wheat RIL populations. Theor Appl Genet, 2014, 127: 659-675.
doi: 10.1007/s00122-013-2249-8 pmid: 24326459
[35] Cuthbert J L, Somers D J, Brule-Babel A L, Brown P D, Crow G H. Molecular mapping of quantitative trait loci for yield and yield components in spring wheat (Triticum aestivum L.). Theor Appl Genet, 2008, 117: 595-608.
doi: 10.1007/s00122-008-0804-5 pmid: 18516583
[36] Hao C Y, Jiao C Z, Hou J, Li T, Liu H X, Wang Y Q, Zheng J, Liu H, Bi Z H, Xu F F, Zhao J, Ma L, Wang Y M, Majeed U, Liu X, Appels R, Maccaferri M, Tuberosa R, Lu H F, Zhang X Y. Resequencing of 145 landmark cultivars reveals asymmetric sub-genome selection and strong founder genotype effects on wheat breeding in China. Mol Plant, 2020, 13: 1733-1751.
doi: 10.1016/j.molp.2020.09.001 pmid: 32896642
[37] Guo W L, Xin M M, Wang Z H, Yao Y Y, Hu Z R, Song W J, Yu K H, Chen Y M, Wang X B, Guan P F, Appels R, Peng H R, Ni Z F, Sun Q X. Origin and adaptation to high altitude of Tibetan semi-wild wheat. Nat Commun, 2020, 11: 5085.
doi: 10.1038/s41467-020-18738-5 pmid: 33033250
[38] Wang J Y, Wang R T, Mao X G, Zhang J L, Liu Y N, Xie Q, Yang X Y, Chang X P, Li C N, Zhang X Y, Jing R L. RING finger ubiquitin E3 ligase gene TaSDIR1-4A contributes to determination of grain size in common wheat. J Exp Bot, 2020, 71: 5377-5388.
doi: 10.1093/jxb/eraa271
[39] Xue Y H, Wang J Y, Mao X G, Li C N, Li L, Yang X, Hao C Y, Chang X P, Li R Z, Jing R L. Association analysis revealed that TaPYL4 genes are linked to plant growth related traits in multiple environments. Front Plant Sci, 2021, 12: 641087.
doi: 10.3389/fpls.2021.641087
[40] Ma Q J, Sun M H, Lu J, Kang H, You C X, Hao Y J. An apple sucrose transporter MdSUT2.2 is a phosphorylation target for protein kinase MdCIPK22 in response to drought. Plant Biotechnol J, 2019, 17: 625-637.
doi: 10.1111/pbi.13003
[41] Deng S Z, Xu L, Xu Z, Lv W Y, Chen Z X, Yang N, Talbot N J, Wang Z Y. A putative PKA phosphorylation site S227 in MoSom1 is essential for infection-related morphogenesis and pathogenicity in Magnaporthe oryzae. Cell Microbiol, 2021, 23: e13370.
[42] Zhuang M J, Li C N, Wang J Y, Mao X G, Li L, Yin J, Du Y, Wang X, Jing R L. The wheat SHORT ROOT LENGTH 1 gene TaSRL1 controls root length in an auxin-dependent pathway. J Exp Bot, 2021, 72: 6977-6989.
doi: 10.1093/jxb/erab357
[43] Zhang B, Xu W N, Liu X, Mao X G, Li A, Wang J Y, Chang X P, Zhang X Y, Jing R L. Functional conservation and divergence among homoeologs of TaSPL20 and TaSPL21, two SBP-Box genes governing yield-related traits in hexaploid wheat. Plant Physiol, 2017, 174: 1177-1191.
doi: 10.1104/pp.17.00113 pmid: 28424214
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