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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (1): 33-40.doi: 10.3724/SP.J.1006.2009.00033

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning of Citrate Synthase Gene in Rapeseed(Brassica napus L.) and Its Expression under Stresses

TONG Jin,ZHAN Gao-Miao, WANG Xin-Fa,LIU Gui-Hua,HUA Wei,WANG Han-Zhong   

  1. Oil Crops Research Institute,Chinese Academy of Agricultural Sciences, Wuhan 430062,China
  • Received:2008-05-06 Revised:2008-07-16 Online:2009-01-12 Published:2008-11-17
  • Contact: WANG Han-Zhong

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Abstract:

As a key enzyme of some metabolisms, citrate synthase shows a sign for metabolism. In order to investigate the function of citrate synthase gene, the cDNA encoding citrate synthase was cloned from rapeseed leaf by RT-PCR. It was 1 659 bp long and encoded a protein with 476 amino acids. The deduced protein sequence had a mitochondrial targeting signal in N-terminal, which was very similar to the citrate synthase in Arabidopsis thaliana (96.0%). Alignment analysis showed that citrate synthase gene had high homology in plants. Under different stresses, we tested the expression of citrate synthase gene in rapeseed leaf by using semi-quantitative PCR. The expression of citrate synthase gene had no obvious change in stresses of salt, dark, high illumination, while was increased at different time in treatments of water logging, drought, IAA, and 6-BA. Interestingly the effect of ABA was contrary to that of IAA. In the treatment of sclerotium blight, the expression of citrate synthase gene was depressed. There was a saddle curve of citrate synthase gene expression in the treatment of gibberellin. The results from real-time PCR of several treatments were mainly in accord with that from semi-quantitative PCR.

Key words: Rapeseed(Brassica napus L.), Citrate synthase, Expression pattern, Stress

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