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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (6): 1146-1150.doi: 10.3724/SP.J.1006.2009.01146

• RESEARCH NOTES • Previous Articles     Next Articles

Drought and Salinity Tolerance in Transgenic Potato Expressing the Betaine Aldehyde Dehydrogenase Gene

ZHANG Ning12,SI Huai-Jun12,LI Liang1,YANG Tao12,ZHANG Chun-Feng1,WANG Di1*   

  1. 1Gansu Key Laboratory of Crop Genetic & Germplasm enhancement,Gansu Agricultural University,Lanzhou 730070,China;2College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China
  • Received:2008-10-29 Revised:2009-02-13 Online:2009-06-12 Published:2009-04-16
  • Contact: WANG Di,E-mail:wangd@gsau.edu.cn

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Abstract:

Glycine betaine (GB) is a common compatible solute in many different organisms including higher plants. Many plant species can accumulate GB in response to drought and salinity. GB is synthesized by conversion of choline to GB through a two-step oxidation via the intermadiate betaine aldehyde. In higher plants, the relevant enzymes are choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH). The fact that many important crops, such as rice, potato and tomato, are betaine-deficient has inevitably led to the proposal that it might be possible to increase drought and salinity tolerances by genetic engineering of GB synthesis. In the present study, the transgenic plants of potato cultivar Gannongshu 2 were obtained by Agrobacterium-mediated transformation of the expression vector pBIBB contained BADH gene under the control of the constitutive promoter CaMV 35S. PCR, Southern and Northern blot analyses showed that the BADH gene was integrated into potato genome, transcribed and expressed in the transgenic plants. The analysis of BADH activity of transgenic plant leaves revealed that the BADH activity ranged from 2 to 11 U, while it was not detectable in the control plants. There was a negative relationship (y= –3.7738x+57.083, r=0.989**) between BADH activity and relative electric conductivity of the transgenic potato leaves. The transgenic potato plants grew normally under NaCl and polyethylene glycol (PEG) stresses with increase of 0.4–0.9 cm for plant height and 17–29% for fresh weight per plant compared with the control plants. This result demonstrated that the transgenic potato plants can improve tolerances to drought and salinity as a result of transformation and expression of BADH gene.

Key words: Potato, Betaine aldehyde dehydrogenase, Genetic transformation, Drought resistance, Salt tolerance

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