Welcome to Acta Agronomica Sinica,

Acta Agronomica Sinica ›› 2009, Vol. 35 ›› Issue (7): 1217-1228.doi: 10.3724/SP.J.1006.2009.01217


Associated Analysis between Temporal and Spatial Expression of Fiber Development Genes and Fiber Quality

JU Ming, WANG Hai-Tang, WANG Li-Ke, LI Fei-Fei, WU Shen-Jie, ZHU Hua-Yu, ZHANG T   

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China
  • Received:2008-10-10 Revised:2009-03-20 Online:2009-07-12 Published:2009-05-19
  • Contact: GUO Wang-Zhen, E-mail: moelab@njau.edu.cn


Ten genes expressed preferentially in fiber development period reported previously and 14 cotton cultivars (strains) with distinctly different fiber quality were selected in this paper. To test the relative expression values of the genes in six different fiber developmental stages, 0 day post anthesis (DPA), 5 DPA, 10 DPA, 15 DPA, 20 DPA, 23 DPA, by real-time quantitative RT-PCR (qRT-PCR), and the data of fiber qualities from 14 cotton varieties (strains). The expression profile showed that GhExp1, GhCIPK1,GhSus1, GhSusA1 and GhPL genes were expressed preferentially during fiber elongation; GhACT1GhRacA and GhRacB genes all had high expression level in earlier stage of fiber elongation and the thickening period of secondary cell wall. Two cellulose synthase genes (GhCelA1 and GhCelA3) were expressed predominantly during late stage of fiber elongation and the thickening period of secondary cell wall. For most genes, the expression value in low expression level period had significant correlation with fiber quality, while no significant correlation was detected in preferential expression stage of these genes with an exception of GhRacA gene. The expression level of GhExp1 in 20DPA fiber of 14 cotton varieties (strains) had a significant negative correlation with fiber strength and uniformity and a significant positive correlation with fiber elongation percentage; the expression level of GhPL gene in 23DPA had a significant negative correlation with fiber length; the expression level of GhRacA gene in 5DPA and 23DPA both had a high significant positive correlation with fiber elongation percentage; the expression level of GhRacB gene in 10DPA had a significant negative correlation with fiber length and uniformity; the expression level of GhCelA1 gene in 5DPA had a significant positive correlation with fiber length and a significant negative correlation with micronaire value; the expression level of GhCelA1 gene in 10DPA had a significant negative correlation with fiber length, a significant positive correlation with micronaire value, and a high significant correlation with fiber elongation percentage; the correlation between the expression levels of GhCIPK1, GhACT1, GhSus1, GhSusA1and GhCelA3 gene and fiber quality indexes had not been detected.

Key words: Cotton, Fiber quality, Genes related with fiber development, Real-time quantitative RT-PCR

[1] Zhang H(张辉), Tang W-K(汤文开), Tan X(谭新), Gong L-L(龚路路), Li X-B(李学宝). Progresses in the study of gene regulation of cotton fiber development. Chin Bull Bot (植物学通报), 2007, 24(2): 127-133(in Chinese with English abstract)

[2] Harmer S E, Orford S J, Timmis J N. Characterisation of six α-expansin genes in Gossypium hirsutum (upland cotton). Mol Genet Genomics, 2002, 268: 1-9

[3] Gao P, Zhao P M, Wang J, Wang H Y, Wu X M, Xia G X. Identification of genes preferentially expressed in cotton fibers: A possible role of calcium signaling in cotton fiber elongation. Plant Sci, 2007, 173: 61-69

[4] Li X B, Fan X P, Wang X L, Cai L, Yang W C. The cotton ACTIN1 gene is functionally expressed in fibers and participates in fiber elongation. Plant Cell, 2005, 17: 859-875

[5] Guo Y(郭媖). Cloning and characterization of five genes related with fiber development in Gossypium hirsutum L. MS Dissertation ofNanjing Agricultural University, 2006(in Chinese with English abstract)

[6] Li X-B(李先碧), Xiao Y-H(肖岳华), Luo M(罗明), Hou L(侯磊), Li D-M( 李德谋), Luo X-Y(罗小英), Pei Y(裴炎). Cloning and expression analysis of two rac genes from cotton (Gossypium hirsutum L.). Acta Genet Sin(遗传学报), 2005, 32(1): 72-78 (in Chinese with English abstract)

[7] Ruan Y L, Prem S C. A fiberless seed mutation in cotton is associated with lack of fiber cell initiation in ovule epidermis and alterations in sucrose synthase expression and carbon partitioning in developing seeds. Plant Physiol, 1998, 118: 399-406

[8] Pear J R, Kawagoe Y, Schreckengost W E, Delmer D P, Stalker D M. Higher plants contain homologs of the bacterial celA genes encoding the catalytic subunit of cellulose synthase. Proc Natl Acad Sci USA, 1996, 93: 12637-12642

[9] Laosinchai W, Cui X, Brown R M Jr. A full cDNA of cotton cellulose synthase has high homology with the Arabidopsis RSW1 gene and cotton CelA1 (accession No. AF 200453) (PGR 00-002). Plant Physiol, 2000, 122: 291

[10]Yu S-W(余舜武), Liu H-Y(刘鸿艳), Luo L-J(罗立军). Analysis of relative gene expression using different real-time quantitative PCR. Acta Agron Sin (作物学报), 2007, 33(7): 1214-1218 (in Chinese with English abstract)

[11] Hughesd D W, Galau G. Preparation of RNA from cotton leaves and pollen. Plant Mol Biol Rep, 1988, 6: 253-257

[12] Wan C Y, Wilkins T A. Isolation of multiple cDNA encoding the vacuolar H+-ATPase subunit B from developing cotton (Gossypium hirsutum L.). Plant Physiol, 1994, 106: 393-394

[13] Pfaffl M W. A new mathematical model for relative quantification in real-time RT-PCR. Nucl Acids Res, 2001, 29: e45

[14] Xu C-N(徐楚年), Yu B-S(余炳生), Zhang Y(张仪), Jia J-Z(贾君镇), Shou Y(寿元). Comparative study of four cultivated cotton species. Beijing Agric Univ J (北京农业大学学报), 1988, 14(2): 113-119(in Chinese with English abstract)

[15] Tang Q-F(汤庆峰), Wen Q-K(文启凯), Tian C-Y(田长彦), Zhang J-S(张巨松), Ma L-C(马黎春). A study process on formation mechanism of cotton fiber quality and its affecting factors. Xinjiang Agric Sci (新疆农业科学), 2003, 40(4): 206-210 (in Chinese with English abstract)

[16] Gou J Y, Wang L J, Chen S P, Hu W L, Chen X Y. Gene expression and metabolite profiles of cotton fiber during cell elongation and secondary cell wall synthesis. Cell Res, 2007, 17: 422-434

[17] Jones M A, Shen J J, Fu Y, Li H, Yang Z B, Grierson C S. The Arabidopsis Rop GTPase is a positive regulator of both root hair initiation and tip growth. Plant Cell, 2002, 14: 763-776

[18] Saxena I M, Brown R M. Cellulose synthase and related enzymes. Curr Opin Plant Biol, 2000, 3: 523-531

[19] Holland N, Holland D, Helentjaris T, Dhugga K S, Xoconostle-Cazares B, Delmer D P. A comparative analysis of the plant cellulose synthase (CesA) gene family. Plant Physiol, 2000, 123: 1313-1324

[20] Delmer D P. Cellulose biosynthesis: Exciting times for a difficult field of study. Annu Rev Plant Physiol Plant Mol Biol, 1999, 50: 245-276
[1] ZHOU Jing-Yuan, KONG Xiang-Qiang, ZHANG Yan-Jun, LI Xue-Yuan, ZHANG Dong-Mei, DONG He-Zhong. Mechanism and technology of stand establishment improvements through regulating the apical hook formation and hypocotyl growth during seed germination and emergence in cotton [J]. Acta Agronomica Sinica, 2022, 48(5): 1051-1058.
[2] SUN Si-Min, HAN Bei, CHEN Lin, SUN Wei-Nan, ZHANG Xian-Long, YANG Xi-Yan. Root system architecture analysis and genome-wide association study of root system architecture related traits in cotton [J]. Acta Agronomica Sinica, 2022, 48(5): 1081-1090.
[3] YAN Xiao-Yu, GUO Wen-Jun, QIN Du-Lin, WANG Shuang-Lei, NIE Jun-Jun, ZHAO Na, QI Jie, SONG Xian-Liang, MAO Li-Li, SUN Xue-Zhen. Effects of cotton stubble return and subsoiling on dry matter accumulation, nutrient uptake, and yield of cotton in coastal saline-alkali soil [J]. Acta Agronomica Sinica, 2022, 48(5): 1235-1247.
[4] ZHENG Shu-Feng, LIU Xiao-Ling, WANG Wei, XU Dao-Qing, KAN Hua-Chun, CHEN Min, LI Shu-Ying. On the green and light-simplified and mechanized cultivation of cotton in a cotton-based double cropping system [J]. Acta Agronomica Sinica, 2022, 48(3): 541-552.
[5] ZHANG Yan-Bo, WANG Yuan, FENG Gan-Yu, DUAN Hui-Rong, LIU Hai-Ying. QTLs analysis of oil and three main fatty acid contents in cottonseeds [J]. Acta Agronomica Sinica, 2022, 48(2): 380-395.
[6] ZHANG Te, WANG Mi-Feng, ZHAO Qiang. Effects of DPC and nitrogen fertilizer through drip irrigation on growth and yield in cotton [J]. Acta Agronomica Sinica, 2022, 48(2): 396-409.
[7] ER Chen, LIN Tao, XIA Wen, ZHANG Hao, XU Gao-Yu, TANG Qiu-Xiang. Coupling effects of irrigation and nitrogen levels on yield, water distribution and nitrate nitrogen residue of machine-harvested cotton [J]. Acta Agronomica Sinica, 2022, 48(2): 497-510.
[8] ZHAO Wen-Qing, XU Wen-Zheng, YANG Liu-Yan, LIU Yu, ZHOU Zhi-Guo, WANG You-Hua. Different response of cotton leaves to heat stress is closely related to the night starch degradation [J]. Acta Agronomica Sinica, 2021, 47(9): 1680-1689.
[9] YUE Dan-Dan, HAN Bei, Abid Ullah, ZHANG Xian-Long, YANG Xi-Yan. Fungi diversity analysis of rhizosphere under drought conditions in cotton [J]. Acta Agronomica Sinica, 2021, 47(9): 1806-1815.
[10] ZENG Zi-Jun, ZENG Yu, YAN Lei, CHENG Jin, JIANG Cun-Cang. Effects of boron deficiency/toxicity on the growth and proline metabolism of cotton seedlings [J]. Acta Agronomica Sinica, 2021, 47(8): 1616-1623.
[11] GAO Lu, XU Wen-Liang. GhP4H2 encoding a prolyl-4-hydroxylase is involved in regulating cotton fiber development [J]. Acta Agronomica Sinica, 2021, 47(7): 1239-1247.
[12] MA Huan-Huan, FANG Qi-Di, DING Yuan-Hao, CHI Hua-Bin, ZHANG Xian-Long, MIN Ling. GhMADS7 positively regulates petal development in cotton [J]. Acta Agronomica Sinica, 2021, 47(5): 814-826.
[13] XU Nai-Yin, ZHAO Su-Qin, ZHANG Fang, FU Xiao-Qiong, YANG Xiao-Ni, QIAO Yin-Tao, SUN Shi-Xian. Retrospective evaluation of cotton varieties nationally registered for the Northwest Inland cotton growing regions based on GYT biplot analysis [J]. Acta Agronomica Sinica, 2021, 47(4): 660-671.
[14] ZHOU Guan-Tong, LEI Jian-Feng, DAI Pei-Hong, LIU Chao, LI Yue, LIU Xiao-Dong. Efficient screening system of effective sgRNA for cotton CRISPR/Cas9 gene editing [J]. Acta Agronomica Sinica, 2021, 47(3): 427-437.
[15] HAN Bei, WANG Xu-Wen, LI Bao-Qi, YU Yu, TIAN Qin, YANG Xi-Yan. Association analysis of drought tolerance traits of upland cotton accessions (Gossypium hirsutum L.) [J]. Acta Agronomica Sinica, 2021, 47(3): 438-450.
Full text



No Suggested Reading articles found!