Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (11): 2008-2014.doi: 10.3724/SP.J.1006.2009.02008

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression Profile Analysis of Ghpg2 Gene Associated with Fertility in Cotton（Gossypium hirsutum L.）

HOU Si-Yu,ZHANG Rui,GUO San-Dui*   

  1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081
  • Received:2009-01-08 Revised:2009-05-30 Online:2009-11-12 Published:2009-08-07
  • Contact: GUO San-Dui, Email: gsdui@mail.caas.net.cn

PDF

1579

Cited

5

Abstract:

Polygalacturonase (PGs) gene family is a kind of proteolytic enzyme in cell wall. A number of higher plants have a higher level of the excision activity at pollen. Polygalacturonase plays an important role on the processes of pollen mature and pollen tube growth. Through BAC library screening, a BAC clone Z55E7 was obtained and congregated with molecular marker STS679 by using PCR strategy. Sequencing analysis results showed that there were six genes in the clone. One of them was a Polygalacturonase gene in cotton, named GhPG2. The expression profiles analysis of this gene would help to know the relationshipof GhPG2 gene with the molecular mechanism of cotton cytoplasm male sterile. GhPG2 gene was isolated from BAC clone. The amino acids and nucleotide sequence of the gene were analyzed by bioinformation software. GhPG2 gene expression levels of different tissues in CMS line, maintainer and restorer line were analyzed by RT-PCR and real-time quantitative PCR method. The gene with the sequence of 1518 bp and an open reading frame of 1239bp coded 412 amino acids and included four conserved domains. Multiple alignment and phylogenetic analysis based on the amino acid sequences showed that GhPG2 was categorized as a gene expressed in pollen. GhPG2 gene expression level in anther, petal and the sixth grade flower bud was higher in the maintainer and restorer lines and lower obviously in the CMS line. And its relative transcript levels were 1.73, 1.45; 3.7, 3.4; 1.63, and 1.39 folds compared with petal, anther and VI flower bud of sterile line, respectively.The gene expression level in anther, petal and the sixth grade flower bud was no significance between the maintainer and restorer line with relative transcript levels were 4.33, 4.08; 13.29, 12.12; 3.25 and 3.10 folds, respectively. It is speculated that GhPG2 gene is of significance in floral organ development in cotton.

Key words: Cotton, GhPG2, Flower Development, Polygalacturonase, Expression profiling

[1] Liu L-C(刘乐承), Zhang T(张弢), Cao J-S(曹家树). Advances in research on molecular biology of plant pollen development. J Yangtze Univ (长江大学学报), 2006, 3(3): 174-178 (in Chinese with English abstract)

[2] Xing C-Z(邢朝柱(in Chinese with English abstract)), Jing S-R(靖深蓉), Xing Y-H(邢以华). Review and prospect on cotton heterosis utilization and study in China. Cotton Sci (棉花学报), 2007, 19(5): 337-345

[3] Feng C D, Stewart J M D, Zhang J F. STS markers linked to the Rf1 fertility restorer gene of cotton. Theor Appl Genet, 2005, 110: 237-243

[4] Yin J M, Guo W Z, Yang L M, Liu L W, Zhang T Z. Physical mapping of the Rf1fertility-restoring gene to a 100 kb region in cotton. Theor Appl Genet, 2006, 112: 1318-1325

[5] Hall B G. Comparison of the accuracies of several phylogenetic methods using protein and DNA sequences. Mol Biol Evol, 2005, 22: 792-802

[6] Wu Y-T(武耀廷), Liu J-Y(刘进元). A modified hot borate method for efficient isolation of total rna from different cotton tissues. Cotton Sci (棉花学报), 2004, 16(2): 67-71 (in Chinese with English abstract)

[7] Jiang P D, Zhang X Q, Zhu Y G, Zhu W, Xie H Y, Wang X D. Metabolism of reactive oxygen species in cotton cytoplasimic male sterility and its restoration. Plant Cell Rep, 2007, 26: 1627-1634

[8] Allen R L, Lonsdale D M. Sequence analysis of three members of the maize polygalacturonase gene family expressed during pollen development. Plant Mol Biol, 1992, 20: 343-345

[9] Chiang J Y, Shu S W, Ko C W, Wang C S. Biochemical characterization of a pollen-specific cDNA encoding polygalacturonase in Lilium longiflorum. Plant Sci, 2006, 170: 433-440
[10] Tebbutt S J, Rogers H J, Lonsdale D M.Characterization of a tobacco gene encoding a pollen-specific polygalacturonase. Plant Mol Biol, 1994, 25: 283-297
[11] Sander L, Child R, Ulvskov P, Albrechtsen M, Borkhardt B. Analysis of a dehiscence zone endo-polygalacturonase in oilseed rape (Brassica napus) and Arabidopsis thaliana: Evidence for roles in cell separation in dehiscence and abscission zones, and in stylar tissues during pollen tube growth. Plant Mol Biol, 2001, 46: 469-479
[12] John M E, Petersen M W. Cotton (Gossypium hirsutum L.) pollen-specific polygalacturonase mRNA: Tissue and temporal specificity of its promoter in transgenic tobacco. Plant Mol Biol, 1994, 26: 1989-1993
[13] Kim J, Shiu S H, Thoma S, Li W H, Patterson S E. Patterns of expansion and expression divergence in the plant polygalacturonase gene family. Genome Biol, 2006, 7: R87
[14] González-Carranza Z H, Elliott K A, Roberts J A. Expression of polygalacturonases and evidence to support their role during cell separation processes in Arabidopsis thaliana. J Exp Bot, 2007, 58: 3719-3730
[15] Cosgrove D J. Expansive growth of plant cell walls. Plant Physiol Biochem, 2000, 38: 109-124
[16] Roberts J A, Elliott K A, Gonzalez-Carranza Z H. Abscission, dehiscence, and other cell separation processes. Annu Rev Plant Biol, 2002, 53: 131-158
[17] Roberts J A, Whitelaw C A, Gonzalez-Carranza Z H, McManus M T. Cell separation processes in plants-models, mechanisms and manipulation. Annl Bot, 2000, 86: 223-235
[18] Patterson S E. Cutting loose: Abscission and dehiscence in Arabidopsis. Plant Physiol, 2001, 126: 494-500
[19] Dubald M, Barakate A, Mandaron P, Mache R. The ubiquitous presence of exopolygalacturonase in maize suggests a fundamental cellular function for this enzyme. Plant J, 1993, 4: 781-791
[20] Torki M, Mandaron P, Mache R, Falconet D. Differential expression of a polygalacturonase gene family in Arabidopsis thaliana. Mol Gen Genet, 1999, 261: 948-952
[21] Zhang Q, Huang L, Liu T T, Yu X L, Cao J S. Functional analysis of a pollen-expressed polygalacturonase gene BcMF6 in Chinese cabbage (Brassica campestris L. ssp. chinensis Makino). Plant Cell Rep, 2008, 27: 1207-1215
[22] Huang L Cao J S, Zhang A H, Ye Y Q, Zhang Y C, Liu T T. The polygalacturonase gene BcMF2 from Brassica campestris is associated with intine development. J Exp Bot, 2009, 60: 301-313
[23] Hadfield K A, Bennett A B. Polygalacturonases: Many genes in search of a function. Plant Physiol, 1998, 117: 337-343
[24] Bussink H J D, Buxton F P, Visser J. Expression and sequence comparison of the Aspergillus niger and Aspergillus tubigensis genes encoding polygalacturonase II. Curr Genet, 1991, 19: 467-474
[25] Robert L S, Allard S, Gerster J L, Cass L, Simmonds J. Isolation and characterization of a polygalacturonase gene highly expressed in Brassica napus pollen. Plant Mol Biol, 1993, 23: 1273-1278
Zhang J F, Turley R B, Stewart J M. Comparative analysis of gene expression between CMS-D8 restored plants and normal non-restoring fertile plants in cotton by differential display. Plant Cell Rep, 2008, 27: 553-561
[1] ZHOU Jing-Yuan, KONG Xiang-Qiang, ZHANG Yan-Jun, LI Xue-Yuan, ZHANG Dong-Mei, DONG He-Zhong. Mechanism and technology of stand establishment improvements through regulating the apical hook formation and hypocotyl growth during seed germination and emergence in cotton [J]. Acta Agronomica Sinica, 2022, 48(5): 1051-1058.
[2] SUN Si-Min, HAN Bei, CHEN Lin, SUN Wei-Nan, ZHANG Xian-Long, YANG Xi-Yan. Root system architecture analysis and genome-wide association study of root system architecture related traits in cotton [J]. Acta Agronomica Sinica, 2022, 48(5): 1081-1090.
[3] YAN Xiao-Yu, GUO Wen-Jun, QIN Du-Lin, WANG Shuang-Lei, NIE Jun-Jun, ZHAO Na, QI Jie, SONG Xian-Liang, MAO Li-Li, SUN Xue-Zhen. Effects of cotton stubble return and subsoiling on dry matter accumulation, nutrient uptake, and yield of cotton in coastal saline-alkali soil [J]. Acta Agronomica Sinica, 2022, 48(5): 1235-1247.
[4] ZHENG Shu-Feng, LIU Xiao-Ling, WANG Wei, XU Dao-Qing, KAN Hua-Chun, CHEN Min, LI Shu-Ying. On the green and light-simplified and mechanized cultivation of cotton in a cotton-based double cropping system [J]. Acta Agronomica Sinica, 2022, 48(3): 541-552.
[5] JIN Rong, JIANG Wei, LIU Ming, ZHAO Peng, ZHANG Qiang-Qiang, LI Tie-Xin, WANG Dan-Feng, FAN Wen-Jing, ZHANG Ai-Jun, TANG Zhong-Hou. Genome-wide characterization and expression analysis of Dof family genes in sweetpotato [J]. Acta Agronomica Sinica, 2022, 48(3): 608-623.
[6] ZHANG Yan-Bo, WANG Yuan, FENG Gan-Yu, DUAN Hui-Rong, LIU Hai-Ying. QTLs analysis of oil and three main fatty acid contents in cottonseeds [J]. Acta Agronomica Sinica, 2022, 48(2): 380-395.
[7] ZHANG Te, WANG Mi-Feng, ZHAO Qiang. Effects of DPC and nitrogen fertilizer through drip irrigation on growth and yield in cotton [J]. Acta Agronomica Sinica, 2022, 48(2): 396-409.
[8] ER Chen, LIN Tao, XIA Wen, ZHANG Hao, XU Gao-Yu, TANG Qiu-Xiang. Coupling effects of irrigation and nitrogen levels on yield, water distribution and nitrate nitrogen residue of machine-harvested cotton [J]. Acta Agronomica Sinica, 2022, 48(2): 497-510.
[9] ZHAO Wen-Qing, XU Wen-Zheng, YANG Liu-Yan, LIU Yu, ZHOU Zhi-Guo, WANG You-Hua. Different response of cotton leaves to heat stress is closely related to the night starch degradation [J]. Acta Agronomica Sinica, 2021, 47(9): 1680-1689.
[10] YUE Dan-Dan, HAN Bei, Abid Ullah, ZHANG Xian-Long, YANG Xi-Yan. Fungi diversity analysis of rhizosphere under drought conditions in cotton [J]. Acta Agronomica Sinica, 2021, 47(9): 1806-1815.
[11] ZENG Zi-Jun, ZENG Yu, YAN Lei, CHENG Jin, JIANG Cun-Cang. Effects of boron deficiency/toxicity on the growth and proline metabolism of cotton seedlings [J]. Acta Agronomica Sinica, 2021, 47(8): 1616-1623.
[12] GAO Lu, XU Wen-Liang. GhP4H2 encoding a prolyl-4-hydroxylase is involved in regulating cotton fiber development [J]. Acta Agronomica Sinica, 2021, 47(7): 1239-1247.
[13] MA Huan-Huan, FANG Qi-Di, DING Yuan-Hao, CHI Hua-Bin, ZHANG Xian-Long, MIN Ling. GhMADS7 positively regulates petal development in cotton [J]. Acta Agronomica Sinica, 2021, 47(5): 814-826.
[14] XU Nai-Yin, ZHAO Su-Qin, ZHANG Fang, FU Xiao-Qiong, YANG Xiao-Ni, QIAO Yin-Tao, SUN Shi-Xian. Retrospective evaluation of cotton varieties nationally registered for the Northwest Inland cotton growing regions based on GYT biplot analysis [J]. Acta Agronomica Sinica, 2021, 47(4): 660-671.
[15] ZHOU Guan-Tong, LEI Jian-Feng, DAI Pei-Hong, LIU Chao, LI Yue, LIU Xiao-Dong. Efficient screening system of effective sgRNA for cotton CRISPR/Cas9 gene editing [J]. Acta Agronomica Sinica, 2021, 47(3): 427-437.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No.12 Zhongguancun South Street, Beijing 100081,China Email:xbzw@chinajournal.net.cn
Supported by Beijing Magtech Co.Ltd