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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (11): 2122-2126.doi: 10.3724/SP.J.1006.2009.02122

• RESEARCH ACTIVITIES • Previous Articles     Next Articles

Nucleotide Polymorphism in the Adh2 of Oryza rufipogon Griff. from Hainan Island

DONG Yi-Bo1,PEI Xin-Wu2,Yuan Qin-Hua3,PENG Yu-Fa1,*   

  1. 1 State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China; 2 Institute of Biotechnology, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 College of Life Science and Agriculture, MOE Key Lab of Tropic Biological Resources, Hainan University, Haikou, 570228, China
  • Received:2009-03-31 Revised:2009-06-25 Online:2009-11-12 Published:2009-08-07
  • Contact: PENG Yu-Fa,E-mail:yfpeng@ippcaas.cn;Tel:010-62815936


The sequence polymorphism of Adh2 in six populations of Oryza rufipogon (WN, WA, WC, DZ, QH, and LD) from Hainan and two Oryza sativa (Nipponbare, 9311) was analyzed. The result showed that there were nine singleton variable sites, four parsimony informative sites, eight synonymous sites and five replacement sites at coding region. There were 21 singleton variable sites, 29 parsimony informative sites and 161 indels sites at intron region. Adh2 polymorphism (pR, qR) at entire region was 0.011. The polymorphism at intron was higher than that at coding region. At coding region, the polymorphism of synonymous sites was higher than that at replacement sites. Neutrality tests showed that there was more purified selection at coding region than at intron region, although no significant difference was found. Cluster analysis showed O. rufipogon from Hainan could be divided into two groups. One group (QH, LD) was clustered with O. rufipogon from China mainland and O. sativa. The other group was clustered with some O. rufipogon from southeast Asia.

Key words: Oryza rufipogon, Adh2, Sequence polymorphism

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