小麦白粉病菌诱导的TaWRKY34基因的鉴定与分析

doi:10.3724/SP.J.1006.2010.00249

Abstract

Abstract:

WRKY transcription factors play important roles in plant defense signaling network. However, little is known about the biological roles of WRKY proteins in wheat(Triticum aestivum L.). The objectives of this study were to screen WRKY transcription factor genes conferring resistance to powdery mildew (Blumeria graminis f. sp. tritici, Bgt) and disclose their function in wheat defense reaction. A WRKY transcription factor gene, TaWRKY34, was identified in response to Bgt by cDNA macroarray and semiquantitative RT-PCR from the wheat full-length cDNA libraries that were constructed in the authors’ earlier studies. This gene encodes 464 amino acid residues. TaWRKY34 was mapped onto short arms of chromosome 1B and 1D through blast search GrainGenes database and homemade full length cDNA library database of Aegilops tauschii. Further experiment indicated that TaWRKY34 also exists on chromosome 1AS through amplifying in Langdon D-genome disomic substitution lines and ChineseSpring nulli-tetrasomic lines with gene specific primers. Examining the subcellular localization of TaWARKY34, its coding region was fused to the 3’ end of green fluorescent protein (GFP). The GFP signal was detected only in the nucleus of onion epidermal cells to transiently express TaWRKY34-GFP, and the control-GFP protein distributed ubiquitously in both nuclei and cytoplasm. This suggests that TaWRKY34 is a nucleus-localized protein. Multiple sequence alignments of 57 WRKY domains from various species indicated that TaWRKY34 is closely related to WRKY transcription factors in response to pathogens in Arabidopsis thaliana (AtWRKY3, AtWRKY4 and AtWRKY33), Hordeum vulgare (HvWRKY42 and HvWRKY46), and Vitis vinifera (VvWRKY2) with identities ranging from 81.8% to 94.5%. Furthermore, TaWRKY34 has similar expression pattern with three sequences from A. thaliana, which was up-regulated at first and then down-regulated when inoculated with pathogens. The expression profiles of TaWRKY34 induced by powdery mildew fungus, salicylic acid and jasmonic acid were different between Pm16/Beijing 837⁷ near-isogenic lines (resistant to Bgt) and Beijing 837 (susceptible to Bgt). The results imply that TaWRKY34 is probably related to the resistance to powdery mildew in wheat.

Key words: Wheat, Blumeria graminis f. sp.tritici, WRKY transcription factor, Chromosome location, Expression pattern

QIN Wei,ZHAO Guang-Yao,QU Zhi-Cai,ZHANG Li-Chao,DUAN Jia-Lei, LI Ai-LI,.

Identification and Analysis of TaWRKY34 Gene Induced by Wheat Powdery Mildew (Blumeria graminis f. sp. tritici)

[J].Acta Agron Sin, 2010, 36(2): 249-255.

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Identification and Analysis of TaWRKY34 Gene Induced by Wheat Powdery Mildew (Blumeria graminis f. sp. tritici)

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