小麦白粉病菌诱导的TaWRKY34基因的鉴定与分析

doi:10.3724/SP.J.1006.2010.00249

Abstract

Abstract:

WRKY transcription factors play important roles in plant defense signaling network. However, little is known about the biological roles of WRKY proteins in wheat(Triticum aestivum L.). The objectives of this study were to screen WRKY transcription factor genes conferring resistance to powdery mildew (Blumeria graminis f. sp. tritici, Bgt) and disclose their function in wheat defense reaction. A WRKY transcription factor gene, TaWRKY34, was identified in response to Bgt by cDNA macroarray and semiquantitative RT-PCR from the wheat full-length cDNA libraries that were constructed in the authors’ earlier studies. This gene encodes 464 amino acid residues. TaWRKY34 was mapped onto short arms of chromosome 1B and 1D through blast search GrainGenes database and homemade full length cDNA library database of Aegilops tauschii. Further experiment indicated that TaWRKY34 also exists on chromosome 1AS through amplifying in Langdon D-genome disomic substitution lines and ChineseSpring nulli-tetrasomic lines with gene specific primers. Examining the subcellular localization of TaWARKY34, its coding region was fused to the 3’ end of green fluorescent protein (GFP). The GFP signal was detected only in the nucleus of onion epidermal cells to transiently express TaWRKY34-GFP, and the control-GFP protein distributed ubiquitously in both nuclei and cytoplasm. This suggests that TaWRKY34 is a nucleus-localized protein. Multiple sequence alignments of 57 WRKY domains from various species indicated that TaWRKY34 is closely related to WRKY transcription factors in response to pathogens in Arabidopsis thaliana (AtWRKY3, AtWRKY4 and AtWRKY33), Hordeum vulgare (HvWRKY42 and HvWRKY46), and Vitis vinifera (VvWRKY2) with identities ranging from 81.8% to 94.5%. Furthermore, TaWRKY34 has similar expression pattern with three sequences from A. thaliana, which was up-regulated at first and then down-regulated when inoculated with pathogens. The expression profiles of TaWRKY34 induced by powdery mildew fungus, salicylic acid and jasmonic acid were different between Pm16/Beijing 837⁷ near-isogenic lines (resistant to Bgt) and Beijing 837 (susceptible to Bgt). The results imply that TaWRKY34 is probably related to the resistance to powdery mildew in wheat.

Key words: Wheat, Blumeria graminis f. sp.tritici, WRKY transcription factor, Chromosome location, Expression pattern

QIN Wei,ZHAO Guang-Yao,QU Zhi-Cai,ZHANG Li-Chao,DUAN Jia-Lei, LI Ai-LI,.

Identification and Analysis of TaWRKY34 Gene Induced by Wheat Powdery Mildew (Blumeria graminis f. sp. tritici)

[J].Acta Agron Sin, 2010, 36(2): 249-255.

References

[1] Eulgem T, Rushton P J, Robatzek S, Somssich I E. The WRKY superfamily of plant transcription factors. Trends Plant Sci, 2000, 5: 199-206

[2] Zhang Y J, Wang L J. The WRKY transcription factor superfamily: Its origin in eukaryotes and expansion in plants. BMC Evol Biol, 2005, 5: 1

[3] Eulgem T, Somssich I E. Networks of WRKY transcription factors in defense signaling. Curr Opin Plant Biol, 2007, 10: 366-371

[4] Kim K C, Lai Z B, Fan B F, Chen Z X. Arabidopsis WRKY38and WRKY62 transcription factors interact with histone deacetylase 19 in basal defense. Plant Cell, 2008, 20: 2357-2371

[5] Liu X Q, Bai X Q, Qian Q, Wang X J, Chen M S, Chu C C. OsWRKY03, a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1. Cell Res, 2005, 15: 593-603

[6] Ryu H S, Han M, Lee S K, Cho J I, Ryoo N, Heu S, Lee Y H, Bhoo S H, Wang G L, Hahn T R, Jeon J S. A comprehensive expression analysis of the WRKY gene superfamily in rice plants during defense response. Plant Cell Rep, 2006, 25:836-847

[7] Cai M, Qiu D Y, Yuan T, Ding X H, Li H J, Duan L, Xu C G, Li X H, Wang S P. Identification of novel pathogen-responsive cis-elements and their binding proteins in the promoter of OsWRKY13, a gene regulating rice disease resistance. Plant Cell Environ, 2008, 31: 86-96

[8] Qiu D Y, Xiao J, Ding X H, Xiong M, Cai M, Cao Y, Li X H, Xu C G, Wang S P. OsWRKY13 mediates rice disease resistance by regulating defense-related genes in salicylate- and jasmonate-dependent signaling. Mol Plant Microbe Interact, 2007, 20: 492-499

[9] Zhang J, Peng Y L, Guo Z J. Constitutive expression of pathogen-inducible OsWRKY31 enhances disease resistance and affects root growth and auxin response in transgenic rice plants. Cell Res, 2008, 18: 508-521

[10] Shimono M, Sugano S, Nakayama A, Jiang C J, Ono K, Toki S, Takatsuji H. Rice WRKY45 plays a crucial role in benzothiadiazole-inducible blast resistance. Plant Cell, 2007, 19: 2064-2076

[11] Liu X Q, Bai X Q, Wang X J, Chu C C. OsWRKY71, a rice transcription factor, is involved in rice defense response. J Plant Physiol, 2007, 164: 969-979

[12] Wang H, Hao J, Chen X, Hao Z, Wang X, Lou Y, Peng Y, Guo Z. Overexpression of rice WRKY89 enhances ultraviolet B tolerance and disease resistance in rice plants. Plant Mol Biol, 2007, 65: 799-815

[13] Mangelsen E, Kilian J, Berendzen K W, Kolukisaoglu U H, Harter K, Jansson C, Wanke D. Phylogenetic and comparative gene expression analysis of barley (Hordeum vulgare) WRKY transcription factor family reveals putatively retained functions between monocots and dicots. BMC Genomics, 2008, 9: 194

[14] Shen Q H, Saijo Y, Mauch S, Biskup C, Bieri S, Keller B, Seki H, Ulker B, Somssich I E, Schulze-Lefert P. Nuclear activity of MLA immune receptors links isolate-specific and basal disease resistance responses. Science, 2007, 315: 1098-1103

[15] Bélanger R R, Benhamou N, Menzies J G. Cytological evidence of an active role of silicon in wheat resistance to powdery mildew (Blumeria graminis f. sp. tritici). Phytopathogy, 2003, 93: 402-412

[16] Bhuiyan N H, Liu W, Liu G S, Selvaraj G, Wei Y, King J. Transcriptional regulation of genes involved in the pathways of biosynthesis and supply of methyl units in response to powdery mildew attack and abiotic stresses in wheat. Plant Mol Biol, 2007, 64: 305-318

[17] Li A L, Wang M L, Zhou R H, Kong X Y, Hou N X, Wang W S, Jia J Z. Comparative analysis of early H₂O₂ accumulation in compatible and incompatible wheat-powdery mildew interactions. Plant Pathol, 2005, 54: 308-316

[18] AbuQamar S, Chen X, Dhawan R, Bluhm B, Salmeron J, Lam S, Dietrich R A, Mengiste T. Expression profiling and mutant analysis reveals complex regulatory networks involved in Arabidopsis response to Botrytis infection. Plant J, 2006, 48: 28-44

[19] Oh S K, Baek K H, Park J M, Yi S Y, Yu S H, Kamoun S, Choi D. Capsicum annuum WRKY protein CaWRKY1 is a negative regulator of pathogen defense. New Phytol, 2008, 177: 977-989

[20] Mzid R, Marchive C, Blancard D, Deluc L, Barrieua F, Corio-Costet M-F, Drirab N, Hamdi S, Lauvergeat V. Overexpression of VvWRKY2 in tobacco enhances broad resistance to necrotrophic fungal pathogens. Physiol Plant, 2007, 131: 434-447

[21] Dellagi A, Heilbronn J, Avrova A O, Montesano M, Palva E T, Stewart H E, Toth I K, Cooke D E L, Lyon G D, Birch P R J. A potato gene encoding a WRKY-like transcription factor is induced in interactions with Erwinia carotovora subsp. atroseptica and Phytophthora infestans and is coregulated with class I endochitinase expression. Mol Plant Microbe Interact,2000, 13: 1092-1101

[22] Chen C H, Chen Z X. Isolation and characterization of two pathogen-and salicylic acid-induced genes encoding WRKY DNA-binding proteins from tobacco. Plant Mol Biol, 2000, 42: 387-396

[23] Larkin M A, Blackshields G, Brown N P, Chenna R. McGettigan P A, McWilliam H, Valentin F, Wallace I M, Wilm A, Lopez R, Thompson J D, Gibson T G, Higgins D G. Clustal W and Clustal X version 2.0. Bioinformatics, 2007, 23: 2947-2948

[24] Clamp M, Cuff J, Searle S M, Barton G J. The Jalview Java alignment editor. Bioinformatics,2004, 20: 426-427

[25] Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol, 2007, 24: 1596-1599

[26] Dong J X, Chen C H, Chen Z X. Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response. Plant Mol Biol, 2003, 51: 21-37

[27] Zheng Z Y, Qamar S A, Chen Z X, Mengiste T. Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens. Plant J, 2006, 48: 592-605

[28] Loake G, Grant M. Salicylic acid in plant defense: The players and protagonists. Curr Opin in Plant Biol, 2007, 10: 466-472

[29] Panstruga R, Parker J E, Schulze-Lefert P. SnapShot: Plant immune response pathways. Cell, 2009, 136: 978.e1-3

[30] Schenk P M, Kazan K, Wilson I. Coordinated plant defense responses in Arabidopsis revealed by microarray analysis. Proc Natl Acad Sci USA, 2000, 97: 11655-11660

[31] Peng J Y, Deng X J, Huang J H, Jia S H, Miao X X, Huang Y P. Role of salicylic acid in tomato (Lycopersicon esculentum) plant defense against cotton bollworm, Helicoverpa armigera Hubner. Z Naturforsch C, 2004, 59: 856-862

[32] Lai Z H, Vinod K M, Zheng Z W, Fan B F, Chen Z X. Roles of Arabidopsis WRKY3 and WRKY4 transcription factors in plant responses to pathogens. BMC Plant Biol, 2008, 8: 68

[33] Robatzek S, Somssich, I E. Targets of AtWRKY6 regulation during plant senescence and pathogen defense. Genes Dev, 2002, 16: 1139-1149

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Identification and Analysis of TaWRKY34 Gene Induced by Wheat Powdery Mildew (Blumeria graminis f. sp. tritici)

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