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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (3): 486-495.doi: 10.3724/SP.J.1006.2010.00486

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Protein Accumulation in Grains of Wheat Cultivars Differing in Drought Tolerance and Its Regulation by Nitrogen Application Amount under Irrigated and Dryland Conditions

SUN Min,GUO Ping-Yi*,GAO Zhi-Qiang,WANG Peng,SHI Jing,MIAO Guo-Yuan   

  1. College of Agronomy,Shanxi Agricultural University,Taigu 030801,China
  • Received:2009-10-26 Revised:2010-01-03 Online:2010-03-12 Published:2010-02-04
  • Contact: GUO Ping-Yi, E-mail: pyguo126@126.com

Abstract:

The protein accumulation in wheat (Triticum aestivum L.) grains is seldom studied under dryland condition although it has been widely tested under irrigated conditions. For understanding the protein accumulation characteristics of wheat cultivars differing in drought tolerance and the effects of water and nitrogen, a drought-tolerant cultivar, Jinmai 47, and a drought-sensitive cultivar, Nongda 189, were planted under both irrigated and dryland conditions with three nitrogen application rates. From the 5th day after anthesis, spikelets and flag leaves were sampled at a 5 days interval for measuring the contents of protein and its components in grains and the enzymes activities in grains and flag leaves. The results indicated that the contents of albumin, gliadin, glutenin, total protein, and the ratio of glutenin to gliadin in dryland cropping were higher than those in irrigated cropping, but the globulin content was lower than that in irrigated cropping. For Nongda 189, the contents of protein and its components and the ratio of glutenin to gliadin in grains were affected significently by drought, whereas for Jinmai 47, the variations on contents of globulin, gliadin, total protein, and ratio of glutenin to gliadin were slightly. The activities of glutamine synthetase (GS), glutamate synthase (GOGAT), glutamic acid-pyruvate transaminase (GPT) in grains and the activities of GS and GOGAT in flag leaves were reduced in dryland cropping compared with those in irrigated cropping. Simultaneously, the trend of GPT activity in grains was also affected by dryland cropping. The effect of drought on activities of these enzymes that involved in protein accumulation was greater in Nongda 189 than in Jinmai 47. Nitrogen application promoted the contents of protein and its components in grains, and higher rate of nitrogen application resulted in greater positive effect. Jinmai 47 presented greater effect of nitrogen application on protein content in grains than Nongda 189. Under both growing conditions, the activities of GS, GOGAT, and GPT in grains and the activity of GOGAT in flag leaves in all nitrogen treatments significantly correlated with the protein yield rather than the protein content in grains. The correlation between GS activity in flag leaves and protein yield in grains was different in the two cultivars. The above results suggest that the drought-tolerant cultivar is less affected by water condition compared with the drought-sensitive cultivar; application of nitrogen could promote the protein content in grains, and the effect is greater in the drought-tolerant cultivar than in the drought-sensitive cultivar.

Key words: Wheat, Drought-tolerant, Irrigated cropping, Dryland cropping, N application amount, Grains protein accumulation

[1]   Deng Z-Y(邓志英), Tian J-C(田纪春), Hu R-B(胡瑞波), Zhou X-F(周晓芳), Zhang Y-X(张永祥). Effect of genotype and environment on wheat main quality characteristics. Acta Ecol Sin (生态学报), 2006, 26(8): 2757-2763 (in Chinese with English abstract)

[2]   Wang X-Z(王宪泽), Tian J-C(田纪春), Zhang Z-Y(张忠义). Study on accumulation laws of the wheat grain protein and its composition. J Shandong Agric Univ (山东农业大学学报), 1994, 25(4): 394-398 (in Chinese with English abstract)

[3]   Daniel C, Triboy E. Changes in wheat protein aggregation during grain development: effects of temperatures and water stress. Eur J Agron, 2002, 16: 1-12

[4]   Zhu X-K(朱新开), Zhou J-L(周君良), Feng C-N(封超年), Guo W-S(郭文善), Peng Y-X(彭永欣). Differences of protein and its component accumulation in wheat for different end uses. Acta Agron Sin (作物学报), 2005,31(3): 342-347 (in Chinese with English abstract)

[5]   Wang X-Y (王小燕), Yu Z-W (于振文). Differences in characteristics of quality and related enzymes activity of different wheat cultivars. Sci Agric Sin (中国农业科学), 2005, 38(10): 1980-1988 (in Chinese with English abstract)

[6]   Day A D, Barmore M A. Effects of soil-moisture stress on the yield and quality of flour from wheat (Triticum aestivum L.). Agron J, 1971, 63: 115-116

[7]   Xu Z-Z(许振柱), Yu Z-W(于振文), Wang D(王东), Zhang Y-L(张永丽). Effect of irrigation conditions on protein composition accumulation of grain and its quality in winter wheat. Acta Agron Sin (作物学报), 2003, 29(5): 682-687 (in Chinese with English abstract)

[8]   Xie Z J, Jiang D, Cao W X, Dai T B, Jing Q. Effects of post anthesis soil water status on the activities of key regulatory enzymes of starch and protein accumulation in wheat grains. J Plant Physiol Mol Biol, 2003, 29: 309-316

[9]   Ma X-M(马新明), Li L(李琳), Zhao P(赵鹏), Xiong S-P(熊淑平), Gou F(郭飞). Effects of water control on activities of nitrogen assimilation enzymes and grain quality in winter wheat. Acta Phytoecol Sin (植物生态学报), 2005, 29(1): 48-53 (in Chinese with English abstract)

[10]Zhao G-C(赵广才), Chang X-H(常旭虹), Liu L-H(刘利华), Yang Y-S(杨玉双), Li Z-H(李振华), Zhou S-Y(周双月), Guo Q-X(郭庆侠), Liu Y-J(刘月洁). Grain Yield and protein components to irrigation in strong gluten wheat. Acta Agron Sin (作物学报), 2007, 33(11): 1828-1833 (in Chinese with English abstract)

[11]Wang Y-(王月福), Yu Z-W(于振文), Li S-X(李尚霞), Yu S-L(余松烈). Effects of soil fertility on protein components content and processing quality in wheat kernel. Acta Bot Boreal-Occident Sin (西北植物学报), 2002, 22(6): 1318-1324 (in Chinese with English abstract)

[12]Wang Y-F(王月福), Yu Z-W(于振文), Li S-X(李尚霞), Yu S-L(余松烈). Effect of soil fertility on free amino acid content and protein content in different organs. J Triticeae Crops (麦类作物学报), 2003, 23(1): 41-43 (in Chinese with English abstract)

[13]Wang Y-F(王月福), Yu Z-W(于振文),Li S-X(李尚霞), Yu S-L(余松烈). Effects of nitrogen application amount on content of protein components and processing quality of wheat grain. Sci Agric Sin (中国农业科学), 2002, 35(9): 1071-1078 (in Chinese with English abstract)

[14]Johansson E, Prieto-Linde M L, Jonsson J O. Efects of wheat cultivar and nitrogen application on storage protein composition and break making quality. Cereal Chem, 2001, 78: 19-25

[15]Fu X-L(付雪丽), Wang C-Y(王晨阳), Guo T-C(郭天财), Zhu Y-J(朱云集), Ma D-Y(马冬云), Wang Y-H(王永华). Effects of water-nitrogen interaction on the contents and components of protein and starch in wheat grains. Chin J Appl Ecol (应用生态学报), 2008, 19(2): 317-322 (in Chinese with English abstract)

[16]Zhao H(赵辉), Jing Q(荆奇), Dai T-B(戴廷波), Jiang D(姜东), Cao W-X(曹卫星). Effects of post-anthesis high temperature and water stress on activities of key regulatory enzymes involved in protein formation in two wheat cultivars. Acta Agron Sin (作物学报), 2007, 33(12): 2021-2027 (in Chinese with English abstract)

[17]Liu P(刘萍), Guo W-S(郭文善), Pu H-C(浦汉春), Feng C-N(封超年), Zhu X-K(朱新开), Peng Y-X(彭永新). Effects of transient high temperature after anthesis on grain protein content and physiological mechanism in wheat (Triticum aestivum L.). Acta Agron Sin (作物学报), 2007, 33(9): 1516-1522 (in Chinese with English abstract)

[18]Fan X-M(范雪梅), Jiang D(姜东), Dai T-B(戴廷波), Jing Q(荆奇), Cao W-X(曹卫星). Effects of post-anthesis drought and waterlogging on the quality of grain formation in different wheat varieties. Acta Phytoecol Sin (植物生态学报), 2004, 28(5): 680-685 (in Chinese with English abstract)

[19]Lin C C, Kao C H. Distributed ammonium assimilation is associated with growth inhibition of roots in rice seedlings caused by NaCl. Plant Growth Regul, 1996, 18: 233-238

[20]Wu L-H(吴良欢), Tao Q-N(陶勤南). Effects of a mino acid-N on rice nitrogen nutrition and its mechanism. Acta Pedol Sin (土壤学报), 2000, 37(4): 464-473 (in Chinese with English abstract)

[21]Jiang D, Yue H X, Wollenweber B, Tan W N, Mu H, Bo Y, Dai T-B, Jing Q, Cao W-X. Effects of post-anthesis drought and waterlogging on accumulation of high-molecular-weight glutenin subunits and glutenin macropolymers content in wheat grain. J Agron Crop Sci, 2009, 195: 89-97

[22]Verma D P S. Control of Gene Expression. Boca. Ratom. Florida. USA: Chemical Rubber Company Press, 1993. pp 425-479

[23]Lea P J, Miflin B.J. Alternative route for nitrogen in higher plants. Nature, 1974, 251: 614-616

Fan X-M(范雪梅), Jiang D(姜东), Dai T-B(戴廷波), Jing Q(荆奇), Cao W-X(曹卫星). Effects of nitrogen rates on activities of key regulatory enzymes for grain starch and protein accumulation in wheat grown under drought and waterlogging from anthesis to maturity. Sci Agric Sin (中国农业科学), 2005, 38(6): 1132-1141 (in Chinese with English abstract)

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