Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (12): 2084-2090.doi: 10.3724/SP.J.1006.2010.02084

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Cloning and Expression Analysis of Four Novel Fatty Acid Synthesis Related Genes in Gossypium hirsutum L.

DONG Jia,WEI Li-Bin,HU Yan,ZHANG Tian-Zhen,GUO Wang-Zhen*   

  1. National Key Laboratory of Crop Genetics & Germplasm Enhancement / Nanjing Agricultural University, Nanjing 210095, China
  • Received:2010-05-24 Revised:2010-08-02 Online:2010-12-12 Published:2010-10-09
  • Contact: GUO Wang-Zhen,E-mail:moelab@njau.edu.cn

PDF

1860

Cited

1

Abstract: Metabolism related to fatty acid synthesis plays an important role both in regulating oil biosynthesis and in plant abiotic stress tolerance. The spatial and temporal expression level of genes related to fatty acid synthesis influences the oil content and enzymes activity for fatty acid synthesis. In this study, four novel genes related to fatty acid synthesis, designated GhKASII, GhKASIII, GhFAD, and GhGPAT, were first isolated from upland cotton, respectively. The cDNA full-length of GhKASIII, GhFAD and GhGPAT were obtained by combining homologic cloning and sillico cloning, while GhKASII was obtained by screening cDNA library and 5'-RACE technique. The expression pattern analysis of different tissues and organs revealed that the transcripts of these genes were widely distributed in all the tested tissues and organs. GhKASII and GhKASIII showed the highest expression level in seeds at 25 DPA (day post anthesis), however, GhGPAT in 0 DPA ovules and 15DPA fiber tissues and GhFAD in 0 DPA ovules, 15 DPA seeds and 20 DPA fibers, respectively. Further, the analysis of expression induced by abiotic treatments indicated that the genes were differentially regulated under wounding, methyl jasmonate (Meja), cold and ABA (abscisic acid) treatments. The study will pave a way to develop further research in oil improvement of cotton seed and resistance to abiotic stress in cotton.

Key words: Fatty acid synthesis, Cloning, Expression, Abiotic stress, Upland cotton

[1]Seki M, Kamei A, Shinozaki K Y. Molecular responses to drought, salinity and frost: common and different paths for plant protection. Curr Opin Biotechnol, 2003, 14: 194–199
[2]Pirtle I L, Kongcharoensuntorn W, Nampaisansuk M, Knesek J E, Chapman K D, Pirtle R M. Molecular cloning and functional expression of the gene for a cotton Δ12 fatty acid desaturase (FAD2). Biochimica et Biophysica Acta, 2001, 2: 122–129
[3]Wolter F P, Schmidt R, Heinz E. Chilling sensitivity of Arabidopsis thaliana with genetically engineered membrane lipids. EMBO Journal, 1992, 11: 4685–4692
[4]Ohlrogge J B, Kuhn D N, Stumpf P K. Subcellular localization of acyl carrier protein in leaf protoplasts of Spinacia oleracea. Proc Natl Acad Sci USA, 1979, 76: 1194–1198
[5]Anai T, Koga M, Tanaka H, Kinoshita T, Rahman S M, Takagi Y. Improvement of rice (Oryza sativa L.) seed oil quality through introduction of a soybean microsomal omega-3 fatty acid desaturase gene. Plant Cell Rep, 2003, 21: 988–992
[6]Wu Y-T(武耀廷), Liu J-Y(刘进元). A modified hot borate method for efficient isolation of total RNA from different cotton tissues. Cotton Sci (棉花学报), 2004, 16(2): 67–71 (in Chinese with English abstract)
[7]Jiang J-X(蒋建雄), Zhang T-Z(张天真). Extraction of total RNA in cotton tissues with CTAB-acidic phenolic method. Cotton Sci (棉花学报), 2003, 15(3): 166–167 (in Chinese with English abstract)
[8]Livak K J, Schmittgen T D. Analysis of relative gene expression data using real­time quantitative PCR and the 2(­delta delta C(T)) method. Methods, 2001, 25: 402–408
[9]Hwang S K, Hwang Y S. Molecular cloning and functional expression of perilla frutescens 3-ketoacyl-
[acyl carrier protein] synthase III. Mol Cells, 2000, 10: 375–381
[10]Hwang S K, Kim K H, Hwang Y S. Molecular cloning and expression analysis of 3-ketoacyl-acp synthases in the immature seeds of Perilla frutescens. Mol cells, 2000, 10: 533–539
[11]Pidkowich M S, Nguyen H T, Heilmann I, Ischebeck T, Shanklin J. Modulating seed β-ketoacyl-acyl carrier protein synthase II level converts the composition of a temperate seed oil to that of a palm-like tropical oil. Proc Natl Acad Sci USA, 2007, 104: 4742–4747
[12]Wanjie S W, Welti R, Moreau R A, Chapman K D. Identi?cation and quanti?cation of glycerolipids in cotton fibers: reconciliation with metabolic pathway predictions from DNA databases. Lipids, 2005, 40: 773–785
[13]Qin Y M, Hu C Y, Pang Y, Kastaniotis A J, Hiltunen J K, Zhu Y X. Saturated very-long-chain fatty acids promote cotton fiber and Arabidopsis cell elongation by activating ethylene biosynthesis. Plant Cell, 2007, 19: 3692–3704
[14]Ji S J, Lu Y C, Feng J X, Wei G, Li J, Shi Y H, Fu Q, Liu D, Luo J C, Zhu Y X. Isolation and analyses of genes preferentially expressed during early cotton fiber development by subtractive PCR and cDNA array. Nucleic Acids Res, 2003, 31: 2534–2543
[15]Nishiuchi T, Hamada T, Kodama H. Wounding changes the spatial expression pattern of the Arabidopsis plastid ω-3 fatty acid desaturase gene (FAD7) through different signal transduction pathways. Plant Cell, 1997, 9: 1701–1712
[16]Hamada T, Nishiuchi T, Kodama H, Nishimura M, Iba K. cDNA cloning of a wounding-inducible gene encoding a plastid ω-3 fatty acid desaturase from tobacco. Plant Cell Physiol, 1996, 37: 606–611
[17]Zou J, Abrams G D, Barton D L, Taylor D C, Pomeroy M K, Abrams S R. lnduction of lipid and oleosin biosynthesis by (+)-abscisic acid and its metabolites in microspore-derived embryos of Brassica napus L. cv. Reston (biological responses in the presence of 8
[prime]-hydroxyabscisic acid). Plant Physiol, 1995, 108: 563–571
[1] CUI Lian-Hua, ZHAN Wei-Min, YANG Lu-Hao, WANG Shao-Ci, MA Wen-Qi, JIANG Liang-Liang, ZHANG Yan-Pei, YANG Jian-Ping, YANG Qing-Hua. Molecular cloning of two maize (Zea mays) ZmCOP1 genes and their transcription abundances in response to different light treatments [J]. Acta Agronomica Sinica, 2022, 48(6): 1312-1324.
[2] CHEN Song-Yu, DING Yi-Juan, SUN Jun-Ming, HUANG Deng-Wen, YANG Nan, DAI Yu-Han, WAN Hua-Fang, QIAN Wei. Genome-wide identification of BnCNGC and the gene expression analysis in Brassica napus challenged with Sclerotinia sclerotiorum and PEG-simulated drought [J]. Acta Agronomica Sinica, 2022, 48(6): 1357-1371.
[3] LI Hai-Fen, WEI Hao, WEN Shi-Jie, LU Qing, LIU Hao, LI Shao-Xiong, HONG Yan-Bin, CHEN Xiao-Ping, LIANG Xuan-Qiang. Cloning and expression analysis of voltage dependent anion channel (AhVDAC) gene in the geotropism response of the peanut gynophores [J]. Acta Agronomica Sinica, 2022, 48(6): 1558-1565.
[4] YAO Xiao-Hua, WANG Yue, YAO You-Hua, AN Li-Kun, WANG Yan, WU Kun-Lun. Isolation and expression of a new gene HvMEL1 AGO in Tibetan hulless barley under leaf stripe stress [J]. Acta Agronomica Sinica, 2022, 48(5): 1181-1190.
[5] ZHOU Hui-Wen, QIU Li-Hang, HUANG Xing, LI Qiang, CHEN Rong-Fa, FAN Ye-Geng, LUO Han-Min, YAN Hai-Feng, WENG Meng-Ling, ZHOU Zhong-Feng, WU Jian-Ming. Cloning and functional analysis of ScGA20ox1 gibberellin oxidase gene in sugarcane [J]. Acta Agronomica Sinica, 2022, 48(4): 1017-1026.
[6] JIN Min-Shan, QU Rui-Fang, LI Hong-Ying, HAN Yan-Qing, MA Fang-Fang, HAN Yuan-Huai, XING Guo-Fang. Identification of sugar transporter gene family SiSTPs in foxtail millet and its participation in stress response [J]. Acta Agronomica Sinica, 2022, 48(4): 825-839.
[7] YUAN Da-Shuang, DENG Wan-Yu, WANG Zhen, PENG Qian, ZHANG Xiao-Li, YAO Meng-Nan, MIAO Wen-Jie, ZHU Dong-Ming, LI Jia-Na, LIANG Ying. Cloning and functional analysis of BnMAPK2 gene in Brassica napus [J]. Acta Agronomica Sinica, 2022, 48(4): 840-850.
[8] ZHOU Yue, ZHAO Zhi-Hua, ZHANG Hong-Ning, KONG You-Bin. Cloning and functional analysis of the promoter of purple acid phosphatase gene GmPAP14 in soybean [J]. Acta Agronomica Sinica, 2022, 48(3): 590-596.
[9] HUANG Cheng, LIANG Xiao-Mei, DAI Cheng, WEN Jing, YI Bin, TU Jin-Xing, SHEN Jin-Xiong, FU Ting-Dong, MA Chao-Zhi. Genome wide analysis of BnAPs gene family in Brassica napus [J]. Acta Agronomica Sinica, 2022, 48(3): 597-607.
[10] JIN Rong, JIANG Wei, LIU Ming, ZHAO Peng, ZHANG Qiang-Qiang, LI Tie-Xin, WANG Dan-Feng, FAN Wen-Jing, ZHANG Ai-Jun, TANG Zhong-Hou. Genome-wide characterization and expression analysis of Dof family genes in sweetpotato [J]. Acta Agronomica Sinica, 2022, 48(3): 608-623.
[11] WU Yan-Fei, HU Qin, ZHOU Qi, DU Xue-Zhu, SHENG Feng. Genome-wide identification and expression analysis of Elongator complex family genes in response to abiotic stresses in rice [J]. Acta Agronomica Sinica, 2022, 48(3): 644-655.
[12] CHEN Xin-Yi, SONG Yu-Hang, ZHANG Meng-Han, LI Xiao-Yan, LI Hua, WANG Yue-Xia, QI Xue-Li. Effects of water deficit on physiology and biochemistry of seedlings of different wheat varieties and the alleviation effect of exogenous application of 5-aminolevulinic acid [J]. Acta Agronomica Sinica, 2022, 48(2): 478-487.
[13] QU Jian-Zhou, FENG Wen-Hao, ZHANG Xing-Hua, XU Shu-Tu, XUE Ji-Quan. Dissecting the genetic architecture of maize kernel size based on genome-wide association study [J]. Acta Agronomica Sinica, 2022, 48(2): 304-319.
[14] YU Hui-Fang, ZHANG Wei-Na, KANG Yi-Chen, FAN Yan-Ling, YANG Xin-Yu, SHI Ming-Fu, ZHANG Ru-Yan, ZHANG Jun-Lian, QIN Shu-Hao. Genome-wide identification and expression patterns in response to signals from Phytophthora infestans of CrRLK1Ls gene family in potato [J]. Acta Agronomica Sinica, 2022, 48(1): 249-258.
[15] YU Guo-Wu, QING Yun, HE Shan, HUANG Yu-Bi. Preparation and application of polyclonal antibody against SSIIb protein from maize [J]. Acta Agronomica Sinica, 2022, 48(1): 259-264.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No.12 Zhongguancun South Street, Beijing 100081,China Email:xbzw@chinajournal.net.cn
Supported by Beijing Magtech Co.Ltd