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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (12): 2084-2090.doi: 10.3724/SP.J.1006.2010.02084

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Cloning and Expression Analysis of Four Novel Fatty Acid Synthesis Related Genes in Gossypium hirsutum L.

DONG Jia,WEI Li-Bin,HU Yan,ZHANG Tian-Zhen,GUO Wang-Zhen*   

  1. National Key Laboratory of Crop Genetics & Germplasm Enhancement / Nanjing Agricultural University, Nanjing 210095, China
  • Received:2010-05-24 Revised:2010-08-02 Online:2010-12-12 Published:2010-10-09
  • Contact: GUO Wang-Zhen,E-mail:moelab@njau.edu.cn

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Abstract: Metabolism related to fatty acid synthesis plays an important role both in regulating oil biosynthesis and in plant abiotic stress tolerance. The spatial and temporal expression level of genes related to fatty acid synthesis influences the oil content and enzymes activity for fatty acid synthesis. In this study, four novel genes related to fatty acid synthesis, designated GhKASII, GhKASIII, GhFAD, and GhGPAT, were first isolated from upland cotton, respectively. The cDNA full-length of GhKASIII, GhFAD and GhGPAT were obtained by combining homologic cloning and sillico cloning, while GhKASII was obtained by screening cDNA library and 5'-RACE technique. The expression pattern analysis of different tissues and organs revealed that the transcripts of these genes were widely distributed in all the tested tissues and organs. GhKASII and GhKASIII showed the highest expression level in seeds at 25 DPA (day post anthesis), however, GhGPAT in 0 DPA ovules and 15DPA fiber tissues and GhFAD in 0 DPA ovules, 15 DPA seeds and 20 DPA fibers, respectively. Further, the analysis of expression induced by abiotic treatments indicated that the genes were differentially regulated under wounding, methyl jasmonate (Meja), cold and ABA (abscisic acid) treatments. The study will pave a way to develop further research in oil improvement of cotton seed and resistance to abiotic stress in cotton.

Key words: Fatty acid synthesis, Cloning, Expression, Abiotic stress, Upland cotton

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