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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (02): 362-368.doi: 10.3724/SP.J.1006.2011.00362

• RESEARCH ACTIVITIES • Previous Articles     Next Articles

Isolation and Expression Analysis of Two GhBlind Homologs in Upland Cotton (Gossypium hirsutum L.)

XIONG Guan-Jun1,XU Qin1,HUA Jin-Ping1,2,*   

  1. 1 College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China;2 Key Laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture / Key Laboratory of Crop Heterosis and Utilization of Ministry of Education / Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
  • Received:2010-06-23 Revised:2010-09-27 Online:2011-02-12 Published:2010-11-16
  • Contact: HUS Jin-Ping, E-mail: jinping_hua@cau.edu.cn

Abstract: In higher plants, Blind homologs play an important role in plant architectures. Two Blind orthologs, GhBlind1 and GhBlind2, were isolated from cDNA of the shoot apex of upland cotton (Gossypium hirsutum L.) by homologous cloning strategy. GenBank accession number is HQ115643 for GhBlind1, and HQ115644 for GhBlind2. Similar to other Blind homologs, GhBlind1 and GhBlind2 were consisted of three exons and two introns, and encoded 359 and 262 amino acids, respectively. Based on the RT-PCR analysis, it was found that GhBlind1 expressed in root, leaf, and tender fiber, and predominantly in shoot apical meristem (SAM) and shoot apex, but not in stem; while GhBlind2 expressed in root, stem, leaf, SAM and shoot apex, but not in tender fiber. Using recombinant PCR technology, expression vectors Psuper-gus-b1 and Psuper-gus-b2 were constructed and used for Agrobacterium-mediated transient expression by transformation of embryogenic callus of upland cotton cv. Coker 201. Histochemical assays of GUS activity showed that after co-cultivation of embryogenic callus for four days, positive events were more obvious in Psuper-gus-b2 than in Psuper-gus-b1. The alignments between GhBlind1, GhBlind2 and the domain of Blind homologs showed the identities of 94% and 91%, respectively. The results of phylogenetic tree showed that GhBlind1 and GhBlind2 were genetically divergent from most of the other R2R3-MYB members in Gossypium hirsutum L. We speculated that GhBlind1 and GhBlind2 are probably related with the development of lateral branch in upland cotton.

Key words: Upland cotton, Blind, Homologous isolation, Expression profile

[1]Wilkins O, Nahal H, Foong J, Provart N J, Campbell M M. Expansion and diversification of the Populus R2R3-MYB family of transcription factors. Plant Physiol, 2009, 149: 981–993
[2]Kranz H D, Denekamp M, Greco R, Jin H, Leyva A, Meissner R, Petroni K, Urzainqui A, Bevan M, Martin C, Smeekens S, Tonelli C, Paz-Ares J, Weisshaar B. Towards functional characterisation of the members of the R2R3-MYB gene family from Arabidopsis thaliana. Plant J, 2001, 16: 263–276
[3]Jin H, Martin C. Multifunctionality and diversity within the plant MYB-gene family. Plant Mol Biol, 1999, 41: 577–585
[4]Stracke R, Werber M, Weisshaar B. The R2R3-MYB gene family in Arabidopsis thaliana. Curr Opin Plant Biol, 2001, 4: 447–456
[5]Jiang C, Gu X, Peterson T. Identification of conserved gene structures and carboxy-terminal motifs in the Myb gene family of Arabidopsis and Oryza sativa L. ssp. indica. Genome Biol, 2004, 5: R46.1-R46.11
[6]Rabinowicz P D, Braun E L, Wolfe A D, Bowen B, Grotewold E. Maize R2R3 Myb genes: Sequence analysis reveals amplification in the higher plants. Genetics, 1999, 153: 427–444
[7]Schmitz G, Tillmann E, Carriero F, Fiore C, Cellini F, Theres K. The tomato Blind gene encodes a MYB transcription factor that controls the formation of lateral meristems. Proc Natl Acad Sci USA, 2002, 99: 1064–1069
[8]Muller D, Schmitz G, Theres K. Blind Homologous R2R3 Myb genes control the pattern of lateral meristem initiation in Arabidopsis. Plant Cell, 2006, 18: 586–597
[9]Liang D, Wong C E, Singh M B, Beveridge C A, Phipson B, Smyth G K, Bhalla P L. Molecular dissection of the pea shoot apical meristem. J Exp Bot, 2009, 60: 4201–4213
[10]Zhang Y-G(张玉刚), Cheng J-H(成建红), Han Z-H(韩振海), Xu X-F(许雪峰), Li T-Z(李天忠). Comparison of methods for total RNA isolation from Malus Xiaojinensis and cDNA LD-PCR amplification. Biotechnol Bull (生物技术通报), 2005, (4): 50–53 (in Chinese with English abstract)
[11]Wang Z-C(王志成), Jiang J-X(蒋建雄), Zhong J(钟军), Yi Z-L(易自力), Liu Q-B(刘清波). Rapid isolation of full-length cDNA sequence from Gossypium hirsutum fiber cDNA library by PCR. J Hunan Agric Univ (湖南农业大学学报), 2005, 31(3): 272–275 (in Chinese with English abstract)
[12]Guo X-Y(郭晓燕). Screening of the Differentially Expressed Genes under Salt Stress in Cotton by Microarray and Construction of the Full Length cDNA Library. MS Dissertation of China Agricultural University, 2010 (in Chinese with English abstract)
[13]Lu X-M(鲁秀敏). Development of an Efficient Agrobacterium-Mediated Transformation System of Cotton Embryogenic Cultures and Generation of Transgenic Cotton with AFP-SPCEMA Genes. MS Dissertation of Southwest Agricultural University, 2003 (in Chinese with English abstract)
[14]Jefferson R A, Burgess S M, Hirsh D. β-Glucuronidase from Escherichia coli as a gene-fusion marker. Proc Natl Acad Sci USA, 1986, 83: 8447–8451
[15]Khuri S, Bakker F T, Dunwell J M. Phylogeny, function, and evolution of the cupins, a structurally conserved, functionally diverse superfamily of proteins. Mol Biol Evol, 2001, 18: 593–605
[16]Schmitz G, Theres K. Genetic control of branching in Arabidopsis and tomato. Curr Opin Plant Biol, 1999, 2: 51–55
[17]Wang Y, Li J. Genes controlling plant architecture. Curr Opin Biotech, 2006, 17: 123–129
[18]Marcotte E M, Pellegrini M, Thompson M J, Yeates T O, Eisenberg D. A combined algorithm for genome-wide prediction of protein function. Nature, 1999, 402: 83–86
[19]Wang S, Wang J, Yu N, Li C, Luo B, Gou J, Wang L, Chen X. Control of plant trichome development by a cotton fiber MYB gene. Plant Cell, 2004, 16: 2323–2334
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