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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (03): 381-388.doi: 10.3724/SP.J.1006.2011.00381

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Mapping QTLs for Seminal Root Architecture and Coleoptile Length in Wheat

LIU Xiu-Lin1,2,CHANG Xiao-Ping2,LI Run-Zhi1,JING Rui-Lian2,*   

  1. 1 Shanxi Agricultural University, Taigu 030801, Shanxi, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Germplasm & Biotechnology, Ministry of Agriculture / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2010-08-18 Revised:2010-12-06 Online:2011-03-12 Published:2011-01-17
  • Contact: 景蕊莲, E-mail: jingrl@caas.com.cn

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Abstract: Root system is important for belowground nutrients acquisition, and is also an important part to respond to drought stress. The purpose of this study was to dissect the genetic basis of seminal root architecture and coleoptile length of wheat by mapping quantitative trait loci (QTLs) of target traits. A doubled haploid (DH) population with 150 lines derived from a cross between two common Chinese wheat varieties Hanxuan 10 and Lumai 14 was used as the plant materials. Gel-chamber was employed to evaluate seminal root architecture traits, including maximum root length (MRL), root number (RN), total root length (TRL), root diameter (RD), root angle (RA), ratio of root dry weight to shoot dry weight (RDW/SDW), and coleoptile length (CL) of seedlings. QTLs for these traits were detected using mixed-model-based composite interval mapping method. A total of 12 additive-effect QTLs and 7 pairs of additive × additive QTLs associated with the target traits were mapped on chromosomes 1A, 1B, 2B, 2D, 3B, 4A, 4D, 5A, 5B, 6A, 7A, and 7B. The phenotypic variation explained by individual additive-effect QTL varied from 5.64% to 12.37%. The additive effects ranged from 0.20 to 7.45. The phenotypic variation explained by each pair of epistatic QTLs varied from 8.70% to 15.90%. Two QTL clusters for seedling root traits were detected on chromosomes 3B and 7A. These results would be helpful to maker-assisted selection of seminal root architecture and coleoptile.

Key words: Wheat, Seminal root architecture, Coleoptile length, QTL, DH lines, Gel-chamber

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