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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (04): 641-649.doi: 10.3724/SP.J.1006.2011.00641

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Cloning and Tissues Expression Analysis of Three Actin Genes from Mulberry (Morus alba)

LI Jun, ZHAO Ai-Chun**, WANG Xi-Lin, ZHANG Qiong-Yu, LI Qi-You, JIN Xiao-Yun, LI Zhen-Gang,YU Mao-De*   

  1. Collage of Biotechnology of Southwest University, Chongqing 400715, China
  • Received:2010-09-13 Revised:2011-01-06 Online:2011-04-12 Published:2011-02-24

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Abstract: Actins play many extremely important roles in plant, but none full-length cDNA actin genes were reported in mulberry until now. Three core fragments, two full CDS and one full-length genomic sequence of actin genes from mulberry were obtained with the strategies of homologous cloning and PCR in this research. Three mulberry actin genes obtained were designated as MaACT1, MaACT2, and MaACT3, respectively. The full-length of MaACT2 was 1 704 bp, which consisted of four exons and three introns, and 377 amino acidswere encoded by 1 134 bp of a putative CDS of MaACT2. The expression profile of three actins in leaf, stem, fruit and root was analyzed by RT-PCR. The results showed that MaACT1 was expressed low in stem, highen with the development of stem and high in young leaf. While MaACT2 and MaACT3 were expressed highly and stably in root, leaf and stem. The results showed MaACT3 was a good candidate for a control gene in the mulberry expression study because it can be expressed highly and stably in different tissues during their development.

Key words: Morus alba, Actin, Gene cloning, Expression analysis, Evolution

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