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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (04): 632-638.doi: 10.3724/SP.J.1006.2012.00632


Genetic Effects of Different RNA Interference Fragments from OsGA20ox2 on Plant Height and Other Agronomic Traits in Rice

WANG Jian1,2, ZHAO Kai-Jun2,*, QIAO Feng2,3,YANG Sheng-Long1   

  1. 1 Crop Institute of Ningxia Academy of Agriculture and Forestry Sciences, Ningxia 750105, China; 2 Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture / National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 College of Life and Geography Sciences, Qinghai Normal University, Xining 810008, China?
  • Received:2011-08-01 Revised:2011-12-19 Online:2012-04-12 Published:2012-02-13
  • Contact: 赵开军, E-mail: zhaokj@mail.caas.net.cn?

Abstract: In our previous study, different rice dwarf lines were obtained through rice genetic transformation using RNAi vectors harboring different fragments of OsGA20ox2 gene. In this study, B1F2 populations were produced by backcrossing between those RNAi dwarf lines and wild-type plants. The progenies of B1F2 populations segregated in a ratio of 3 dwarf plants to 1 normal height plant, indicating that dwarf trait was dominantly inherited. At the same time, plant height, the internode length and some major agronomic traits of dwarf plants of F5 and B1F2 populations were analyzed. It was resulted that RNAiof OsGA20ox2 gene significantly reduced the rice plant height and the internode length. The longer the interference fragment was, the shorter the plant height and internode length were. In general, the rice plant height was reduced by 24–42 cm, dwarfing ratio was 22–39%. For the same RNAi fragment, the average shorten length of the first internode (counting from the top to bottom) was very similar to that of the second, and the third was very similar to the fourth. The dwarfing ratio of each internode showed a trend of the fourth > third > second > first section. Bottom internodes obtained a greater dwarfing ratio, which is good for improving lodging resistance of rice, and the upper internodes showed a lower dwarfing ratio that made the plant maintain a reasonable height, which helps the rice plants to remain a stable and high yield. Other major agronomic traits such as grain weight, seed setting rate, panicle length were not significantly affected by the RNAiof OsGA20ox2 gene.

Key words: RNAi, Plant height, Length of internode, Genetic effect

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