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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (05): 914-920.doi: 10.3724/SP.J.1006.2012.00914

• RESEARCH NOTES • Previous Articles     Next Articles

Identification and Genetic Analysis of Hybrid from Cross between Erianthus arundinacius (Retz.) Jesws. and Saccharum spontaneum L.

LIU Xi-Hui,FANG Feng-Xue,GAO Yi-Jing,ZHANG Rong-Hua,SONG Huan-Zhong,YANG Rong-Zhong,FANG Wei-Kuan,DUAN Wei-Xing,LUO Ting, ZHANG Ge-Min*,LI Yang-Rui*   

  1. Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences / Guangxi Key Laboratory of Sugarcane Genetic Improvement / Sugarcane Research Center, Chinese Academy of Agricultural Sciences / Guangxi Sugarcane Variety Branch, National Sugar Crops Improvement Center / State and Province Joint Research Center for Sugarcane Breeding and Cultivation Technologies, Nanning 530007, China
  • Received:2011-07-25 Revised:2012-01-19 Online:2012-05-12 Published:2012-03-05
  • Contact: 张革民, E-mail: zhanggm68@gxaas.net; 李杨瑞, E-mail: liyr@gxaas.net E-mail:liuxihui@gxaas.net

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Abstract: Hybridization between different populations is the most common means to enrich genetic variation. Studies on genetic differentiation and characterizations of hybrid are crucial to protect and evaluate genetic resources. In this paper, the hybrid from Erianthus arundinacius (Retz.)Jesws. × Saccharum spontaneum L. were analyzed using SSR and SRAP markers. The results showed that the hybrid GXAS07-6-1 was the true hybrid identified by using three pairs of SSR primers and five pairs of SRAP primers. A total of 1 095 DNA bands were amplified by 71 pairs of selective SRAP primers. Among 1 095 genetic loci, 800 were polymorphic. Therefore, the average polymorphic ratio was 73.0% and each pair of SRAP primers yielded 11 polymorphic loci on average. The genetic relationship between the hybrid and its parents analyzed with software NTSYS-pc2.10e showed that the genetic similarity coefficient was 0.474 between the parents, while 0.696 between the hybrid and the female parent and 0.752 between the hybrid and the male parent, suggesting that the hybrid GXAS07-6-1 was more inherited from male parent.

Key words: Erianthus arundinacius (Retz.) Jesws., Saccharum spontaneum L., Hybrid identification, SSR, SRAP

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