Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (05): 914-920.doi: 10.3724/SP.J.1006.2012.00914

• RESEARCH NOTES • Previous Articles     Next Articles

Identification and Genetic Analysis of Hybrid from Cross between Erianthus arundinacius (Retz.) Jesws. and Saccharum spontaneum L.

LIU Xi-Hui,FANG Feng-Xue,GAO Yi-Jing,ZHANG Rong-Hua,SONG Huan-Zhong,YANG Rong-Zhong,FANG Wei-Kuan,DUAN Wei-Xing,LUO Ting, ZHANG Ge-Min*,LI Yang-Rui*   

  1. Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences / Guangxi Key Laboratory of Sugarcane Genetic Improvement / Sugarcane Research Center, Chinese Academy of Agricultural Sciences / Guangxi Sugarcane Variety Branch, National Sugar Crops Improvement Center / State and Province Joint Research Center for Sugarcane Breeding and Cultivation Technologies, Nanning 530007, China
  • Received:2011-07-25 Revised:2012-01-19 Online:2012-05-12 Published:2012-03-05
  • Contact: 张革民, E-mail: zhanggm68@gxaas.net; 李杨瑞, E-mail: liyr@gxaas.net E-mail:liuxihui@gxaas.net

PDF

1585

Cited

5

Abstract: Hybridization between different populations is the most common means to enrich genetic variation. Studies on genetic differentiation and characterizations of hybrid are crucial to protect and evaluate genetic resources. In this paper, the hybrid from Erianthus arundinacius (Retz.)Jesws. × Saccharum spontaneum L. were analyzed using SSR and SRAP markers. The results showed that the hybrid GXAS07-6-1 was the true hybrid identified by using three pairs of SSR primers and five pairs of SRAP primers. A total of 1 095 DNA bands were amplified by 71 pairs of selective SRAP primers. Among 1 095 genetic loci, 800 were polymorphic. Therefore, the average polymorphic ratio was 73.0% and each pair of SRAP primers yielded 11 polymorphic loci on average. The genetic relationship between the hybrid and its parents analyzed with software NTSYS-pc2.10e showed that the genetic similarity coefficient was 0.474 between the parents, while 0.696 between the hybrid and the female parent and 0.752 between the hybrid and the male parent, suggesting that the hybrid GXAS07-6-1 was more inherited from male parent.

Key words: Erianthus arundinacius (Retz.) Jesws., Saccharum spontaneum L., Hybrid identification, SSR, SRAP

[1]Zhang M-Q(张木清), Hong Y-X(洪艺珣), Li Q-W(李奇伟), Liu S-M(刘少谋), Zhang C-M(张垂明), Yang R-Z(杨荣仲). Molecular polymorphic analyses for the germplasms of Erianthus arundinaceus collected in China. J Plant Resour Environ (植物资源与环境学报), 2004, 13(1): 1–6 (in Chinese with English abstract)

[2]Zhang G-M(张革民), Yang R-Z(杨荣仲), Liu H-B(刘海斌). Principal component analysis for quantitative traits and cluster analysis based on quantitative traits of Saccharum spontaneum L. Southwest China J Agric Sci (西南农业学报), 2006, 19(6): 1127–1131 (in Chinese with English abstract)

[3]Zhang G-M(张革民), Liao J-X(廖江雄), Huang H-T(黄宏套), Li H-G(黎焕光), Rong F-Y(容凤玉), Yang R-Z(杨荣仲), Fang W-K(方位宽), Bi S-L(闭少玲), Xian W(贤武), Tan F(谭芳). Genetic diversity of Saccharum spontaneum L. with high sugar content in Guangxi based on phenotypic traits and RAPD markers. J Southwest Univ (Nat Sci Edn)(西南大学学报?自然科学版), 2007, 29(8): 83–88 (in Chinese with English abstract)

[4]Zhou Y-H(周耀辉), Huang H-N(黄鸿能), Yin P-J(殷培军), Fu C(符城), Yang Y-H(杨业后). Studies on characteristic of sugarcane germplasm at Hainan sugarcane breeding station. Sugarcane Canesugar (甘蔗糖业), 1997, (3): 2–7 (in Chinese with English abstract)

[5]Zhou Y-H(周耀辉), Huang H-N(黄鸿能), Chen J-P(陈建平), Yang Y-H(杨业后). Fu C(符城), Ji J-L(吉家乐). Studies on characteristic of sugarcane germplasm at Hainan sugarcane breeding station (III). Sugarcane Canesugar (甘蔗糖业), 1999, (2): 1–9 (in Chinese with English abstract)

[6]Huang J-Y(黄家雍), Liao J-X(廖江雄), Zhu-Ge Y(诸葛莹). Intergeneric copulatality of Saccharum L. with Narenga porphyrocoma, Miscamthus floridulus and Erianthus kockii, the morphology and isozyme analysis of their hybrid F1 clones. Southwest China J Agric Sci (西南农业学报), 1997, 10(3): 92–98 (in Chinese with English abstract)

[7]Zheng X-F(郑雪芳), Zhang M-Q(张木清), Li Q-W(李奇伟), Lao F-X(劳方业), Deng Z-H(邓祖湖), Chen R-K(陈如凯), Yang Y-H(杨业后). Utilization and characterisation of the genuine intergeneric hybrids from the cross of Saccharum and E. arundinaceum: (2) Molecular identification of genuine hybrids from the cross of Saccharum and E. arundinaceum. Mol Plant Breed (分子植物育种), 2004, 2(1): 35–42 (in Chinese with English abstract)

[8]D’Hont A, Rao P S, Feldmann P, Berding N, Glaszmann J C. Identification and characterization of intergeneric hybrids, Saccharum officinarum × Erianthus arundinaceus, with molecular markers and in situ hybridization. Theor Appl Genet, 1995, 91: 320–326

[9]Cai Q, Aitken K, Deng H H, Chen X W, Fu C, Jackson P A, Mcintye C L. Verification of the introgression of Erianthus arundinaceus germplasm into sugarcane using molecular markers. Plant Breed, 2005, 124: 322–328

[10]You J-H(游建华), Wu K-C(吴凯朝), Liang J(梁俊), Fang F-X(方锋学), Mo L-X(莫磊兴). Assessment of the genetic diversity among sugarcane cultivars by using SSR marker. J Anhui Agric Sci (安徽农业科学), 2008, 36(10): 3990–3992 (in Chinese with English abstract)

[11]Liang J(梁俊), Li Y-R(李杨瑞), Fang F-X(方锋学), Wu K-C(吴凯朝), You J-H(游建华). Assessment of genetic diversity in Saccharum using SSR markers and capillary electrophoresis. Guihaia (广西植物), 2010, 30(1): 106–111 (in Chinese with English abstract)

[12]Liu X-L(刘新龙), Ma L(马丽), Chen X-K(陈学宽), Ying X-M(应雄美), Cai Q(蔡青), Liu J-Y(刘家勇), Wu C-W(吴才文). Establishment of DNA fingerprint ID in sugarcane cultivars in Yunnan, China. Acta Agron Sin (作物学报), 2010, 36(2): 202–210 (in Chinese with English abstract)

[13]Li Y-Y(李媛媛), Shen J-X(沈金雄), Wang T-H(王同华), Fu Y-D(傅延栋), Ma C-Z(马朝芝). Construction of a linkage map using SRAP, SSR and AFLP markers in Brassica napus L. Sci Agric Sin (中国农业科学), 2007, 40(6): 1118–1126 (in Chinese with English abstract)

[14]Liu X-H(刘昔辉), Song H-Z(宋焕忠), Zhang G-M(张革民), Zhang R-H(张荣华), Fang W-K(方位宽), Ou H-P(区惠平), Lu J-X(陆建勋), Liang Q(梁强), Tang H-Q(唐红琴). Rapid and efficient method for extracting genomic DNA from Sugarcane and its related genera. Southwest China J Agric Sci (西南农业学报), 2010, 23(2): 515–518 (in Chinese with English abstract)

[15]Huang D-L(黄东亮), Qin X-L(覃肖良), Liao Q(廖青), Gao Y-J(高轶静), Fang F-X(方锋学). Simple and rapid procedure for isolation of high quality genomic DNA from sugarcane. Biotechnol Bull (生物技术通讯), 2010, (5): 101–106 (in Chinese with English abstract)

[16]Pan Y B. Highly polymorphic microsatellite DNA markers for sugarcane germplasm evaluation and variety identity testing. Sugar Tech, 2006, 8: 246–256

[17]Liu X-H(刘昔辉), Zhang G-M(张革民), Song H-Z(宋焕忠), Fang W-K(方位宽), Ou H-P(区惠平). Optimization of SSR-PCR system for S. spontanuem L. J Southwest Univ (Nat Sci Edn) (西南大学学报•自然科学版) 2010, 32(6): 11–15 (in Chinese with English abstract)

[18]Cai Q(蔡青), Wen J-C(文建成), Fan Y-H(范源洪), Wang L-P(王丽萍), Ma L(马丽). Chromosome analysis of Saccharum L. and related plants. Southwest China J Agric Sci (西南农业学报), 2002, 15(2): 16–19 (in Chinese with English abstract)

[19]He F-F(何凤发), Yang Z-P(杨志平), Zhang Z-J(张正坚), Wang G-X(王贵学), Wang J-C(王季春). Genetic diversity analysis of potato germplasm by SRAP makers. J Agric Biotechnol (农业生物技术学报), 2007, 15(6): 1001–1005 (in Chinese with English abstract)

[20]Li X-H(李晓慧), Tian C-Y(田朝阳), Wang C-Y(王从彦). Genetic polymorphism analysis by sequence-related amplified polymorphism markers in Citrullus lanatus. Biotechnology (生物技术), 2007, 17(3): 23–26 (in Chinese with English abstract)

[21]Chen L-J(陈丽静), Pan G-G(潘刚刚), He J(何晶), Liu J(刘君), Zhang L-J(张立军), Zhang L(张丽). The classification of maize inbred lines by SRAP marker technology. J Sichuan Agric Univ (四川农业大学学报), 2009, 27(2):148–152 (in Chinese with English abstract)

[22]Lalitha R, Premachandran M N. Meiotic abnormalities in intergeneric hybrids between Saccharum spontaneum and Erianthus arundinaceus (gramineae). Jpn Mendel Soc, 2007, 72: 337–343

[23]Alwala S, Kimbeng C A, Veremis J C, Gravois K A. Identification of molecular markers associated with sugar-related traits in a Saccharum interspecific cross. Euphytica, 2009, 167:127–142

[24]Suman A, Kimbeng C A, Edme S J, Veremis J. Sequence-related amplified polymorphism (SRAP) markers for assessing genetic relationships and diversity in sugarcane germplasm collections. Plant Genet Resour: Character Utiliz, 2008, 6: 222–231
[1] Zhang Ying-Xing, Bheel Chander Kumar, Song Yu-Zhen, Wang Yue, Cao Yue, Khound Rituraj, Santra Dipak Kumar, Cao Xiao-Ning, Wang Rui-Yun. Screening and phenotypic characterization of EMS-induced mutants with elite agronomic traits in broomcorn millet [J]. Acta Agronomica Sinica, 2026, 52(5): 1388-1400.
[2] Tian Chun-Yan, Lu Xin, Wu Cai-Wen, Xu Chao-Hua, Liu Jia-Yong, Bian Xin, Tao Lian-An. Genetic diversity analysis and breeding potential evaluation of innovative sugarcane germplasm based on fluorescent SSR [J]. Acta Agronomica Sinica, 2026, 52(4): 1057-1072.
[3] WANG Hao-Chen, WANG Ke-Jing, HAN Juan, LI Xiang-Hua. Spatial genetic structure characteristics of natural populations of Glycine tomentella in two representative habitats in the southeast coast, China: a study of intrapopulation sampling strategy [J]. Acta Agronomica Sinica, 2025, 51(11): 2875-2885.
[4] ZHENG Dong, ZHOU Xian-Li, TENG Chang-Cai, HOU Wan-Wei, ZHANG Hong-Yan, LIU Yu-Jiao. Genetic relationship analysis and fingerprints construction of faba bean varieties in Qinghai province based on SSR markers [J]. Acta Agronomica Sinica, 2025, 51(1): 79-90.
[5] KUANG Bo-Wen, WEI Ni, LIU Jin-Dian, CHEN Mei-Yan, MAO Xing-Jie, DUAN Wei-Xing, YANG Xi-Ping. Development of SSR markers and database based on genomes of sugarcane and its relatives [J]. Acta Agronomica Sinica, 2025, 51(1): 103-116.
[6] DING Yi-Bing, XIN Xu-Xia, FENG Zhi-Zun, GUO Juan, CAO Yue, CHEN Xi-Ming, WANG Xiao-Dan, CAO Xiao-Ning, SANTRA Dipak K, CHEN Ling, QIAO Zhi-Jun, WANG Rui-Yun. Core germplasm identification of proso millet in spring sowing area of Northeast China using fluorescent microsatellite markers [J]. Acta Agronomica Sinica, 2024, 50(7): 1728-1739.
[7] DING Yi-Bing, XIN Xu-Xia, FENG Zhi-Zun, CAO Yue, GUO Juan, Dipak K SANTRA, WANG Rui-Yun, CHEN Xi-Ming. Core germplasm and DNA molecular identity card of proso millet in Northeast Spring sowing region in China [J]. Acta Agronomica Sinica, 2024, 50(5): 1181-1192.
[8] TIAN Chun-Yan, BIAN Xin, LANG Rong-Bin, YU Hua-Xian, TAO Lian-An, AN Ru-Dong, DONG Li-Hua, ZHANG Yu, JING Yan-Fen. Association analysis of three breeding traits with SSR markers and exploration of elite alleles in sugarcane [J]. Acta Agronomica Sinica, 2024, 50(2): 310-324.
[9] CHEN Tian, LI Yu-Ying, RONG Er-Hua, WU Yu-Xiang. Character identification and floral organ transcriptome analysis on artificial allotetraploids of Gossypium hirsutum L. [J]. Acta Agronomica Sinica, 2024, 50(2): 325-339.
[10] CHEN Zhi-Kai, ZHOU Xian-Li, ZHANG Hong-Yan, TENG Chang-Cai, HOU Wan-Wei. SSR association analysis of the protein content of 320 faba bean germplasms [J]. Acta Agronomica Sinica, 2024, 50(11): 2775-2786.
[11] CAO Yue, Zhang Li-Yuan, XIN Xu-Xia, FENG Zhi-Zun, GUO Juan, WANG Xiao-Dan, CAO Xiao-Ning, SANTRA Dipak K, CHEN Ling, QIAO Zhi-Jun, WANG Rui-Yun. Construction of DNA molecular identity card of proso millet in Ningxia based on fluorescent SSR [J]. Acta Agronomica Sinica, 2024, 50(11): 2699-2711.
[12] GUO Juan, XIN Xu-Xia, FENG Zhi-Zun, CAO Yue, WANG Xiao-Dan, CAO Xiao-Ning, SANTRA Dipak K, CHEN Ling, QIAO Zhi-Jun, WANG Rui-Yun. Molecular identification of broomcorn millet germplasm resources in Shaanxi based on SSR [J]. Acta Agronomica Sinica, 2024, 50(10): 2643-2653.
[13] WANG Qian, ZHANG Li-Yuan, XU Yue, LI Hai, LIU Shao-Xiong, XUE Ya-Peng, LU Ping, WANG Rui-Yun, LIU Min-Xuan. High motif EST-SSR markers development and genetic diversity evaluation for 200 core germplasms in proso millet [J]. Acta Agronomica Sinica, 2023, 49(8): 2308-2318.
[14] CHEN Yi-Hang, TANG Chao-Chen, ZHANG Xiong-Jian, YAO Zhu-Fang, JIANG Bing-Zhi, WANG Zhang-Ying. Construction of core collection of sweetpotato based on phenotypic traits and SSR markers [J]. Acta Agronomica Sinica, 2023, 49(5): 1249-1261.
[15] LI Ying, LIU Hai-Cui, SHI Lyu, SHI Xiao-Xu, HAN Xiao, LIU Jian, WEI Ya-Feng. Genetic diversity and population structure analysis of naked barley germplasm resources in Jiangsu province [J]. Acta Agronomica Sinica, 2023, 49(10): 2687-2697.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd