Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (08): 1369-1377.doi: 10.3724/SP.J.1006.2012.01369

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Construction of Genetic Map and Analysis of QTLs for Grain Weight Using a RIL Population Derived from Shannong 01-35 × Gaocheng 9411

SHI Cui-Lan,ZHENG Fei-Fei,CHEN Jian-Sheng,HAN Shu-Xiao,WANG Yong-Rui,TIAN Ji-Chun*   

  1. State Key Laboratory of Crop Biology / Shandong Provincial Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China
  • Received:2012-01-09 Revised:2012-04-20 Online:2012-08-12 Published:2012-06-04
  • Contact: 田纪春, E-mail: jctian@sdau.edu.cn; Tel: 0538-8242040

Abstract: The objectives of this study were to construct a new linkage map of wheat using a recombinant inbred line (RIL) population derived from the cross between Shannong 01-35 and Gaocheng 9411 and locate quantitative trait loci for thousand-grain weight (TGW) using this map. The RIL population, consisting of 182 lines, was obtained via single-seed descendent method until the F8 generation. The genetic map consisted of one TaGW2-CAPS,59 SSR, and442 DArT markers in 29 linkage groups, including 54 novel markers (44 DArT markers and 10 SSRs) assigned into 18 linkage groups. The total genetic length of the map was 4084.5 cM with an average interval distance of 8.13 cM. The 182 RILs and their parents were grown in four environments from 2008 to 2010, and QTLs associated with TGW were identified using mixed linear model based on both separated and joint environments. A total of seven QTLs were detected including three QTLs (QGW4B-7, QGW5B-20, and QGW6A-29) commonly found using both methods. Three major QTLs, i.e., QGW4B-7, QGW5B-12, and QGW6A-29, exhibited phenotypic contributions higher than 10%. These results suggest that the QTLs detected by using the successfully constructed genetic map are valuable in molecular marker-assisted selection in wheat.

Key words: Wheat, Genetic map, Grain weight, QTL

[1]Röder M S, Korzun V, Wendehake K, Plaschke J, Tixier M H, Leroy P, Ganal M W. A microsatellite map of wheat. Genetics, 1998, 149: 2007-2023

[2]Somers D J, Isaac P, Edwards K. A high density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet, 2004, 109: 1105-1114

[3]Torada A, Koike M, Mochida K, Ogihara Y. SSR-based linkage map with new markers using an intraspecific population of common wheat. Theor Appl Genet, 2006, 112: 1042-1051

[4]Liu G(刘刚), Xu S-B(许盛宝), Ni Z-F(倪中福), Li J(李晶), Qin D-D(秦丹丹), Dou B-D(窦秉德), Peng H-R(彭慧茹), Sun Q-X(孙其信). Genetic analysis of segregation distortion of molecular markers in wheat RIL population. J Agric Biotechnol (农业生物技术学报), 2007, 15(5): 828-833 (in Chinese with English abstract)

[5]Shi P-C(石培春), Wang G-L(王光利), Zhang W(张薇), Cao L-P(曹连莆). Construction of wheat SSR molecular linkage map and its polymorphism. Xinjiang Agric Sci (新疆农业科学), 2007, 44(suppl-3): 71-76 (in Chinese with English abstract)

[6]Zhang K P, Zhao L, Tian J C, Chen G F, Jiang X L, Liu B. A genetic map conducted using a doubled haploid population derived from two elite Chinese common wheat (Triticum aestivum L.) varieties. J Integr Plant Biol, 2008, 50: 941-950

[7]Jaccoud D, Peng K, Feinstein D, Kilian A. Diversity arrays: a solid state technology for sequence information independent genotyping . Nucl Acids Res, 2001, 29(4): e25

[8]Wenzl P, Carling J, Kudrna D, Jaccoud D, Huttner E, Kleinhofs A, Kilian A. Diversity arrays technology (DArT) for whole genome profiling of barley. Proc Natl Acad Sci USA, 2004, 101: 9915-9920

[9]Hong Y-H(洪义欢), Xiao-Y(肖宁), Zhang C(张超), Su Y(苏琰), Chen J-M(陈建民). Principle of diversity arrays technology (DArT) and its applications in genetic research of plants. Hereditas (遗传), 2009, 31(4): 359-364 (in Chinese with English abstract)

[10]Hearnden P R, Eckermann P J, McMichael G L, Hayden M J, Eglinton J K, Chalmers K J. A genetic map of 1000 SSR and DArT markers in a wide barley cross. Theor Appl Genet, 2007, 115: 383-391

[11]Mantovani P, Wenzl P, Catizone I, Huttner E, Maccaferri M, Corneti S, Sanguineti M C, Tuberosa R, Deambrogio E, Kilian A. A durum wheat linkage map: integration of SSR and DArT markers. In: Proceedings of the 51st Italian Society of Agricultural Genetics Annual Congress, Riva del. Garda, Italy, 2007

[12]Wenzl P, Li H B, Carling J, Zhou M X, Raman H, Paul E, Hearnden P, Maier C, Xia L, Caig V, Ovesná J, Cakir M, Poulsen D, Wang J P, Raman R, Smith K P, Muehlbauer G J, Chalmers K J, Kleinhofs A, Huttner E, Kilian A. A high density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits. BMC Genomics, 2006, 7: 206-227

[13]Huynh B L , Wallwork H, Stangoulis J C R, Graham R D, Willsmore K L, Olson S, Mather D E. Quantitative trait loci for grain fructan concentration in wheat (Triticum aestivum L.). Theor Appl Genet, 2008, 117: 701-709

[14]Semagn K, Bjornstad A, Skimes H, Maroy A G, Tarkegne Y, William M. Distribution of DArT, AFLP and SSR markers in a genetic linkage map of a doubled haploid hexaploid wheat population. Genome, 2006, 49: 545-555

[15]Griffiths S, Simmonds J, Leverington M, Wang Y K, Fish L, Sayers L, Alibert L, Orford S, Wingen L, Herry L, Faure S, Laurie D, Bilham L, Snape J. Meta-QTL analysis of the genetic control of ear emergence in elite European winter wheat germplasm. Theor Appl Genet, 2009, 119: 383-395

[16]Yao Q(姚琴), Zhou R-H(周荣华), Pan Y-M(潘昱名), Fu T-H(傅体华), Jia J-Z(贾继增). Construction of genetic linkage map and QTL analysis of agronomic important traits based on a RIL population derived from common wheat variety Yanzhan 1 and Zaosui 30. Sci Agric Sin (中国农业科学), 2010, 43(20): 4130-4139 (in Chinese with English abstract)

[17]Su Z-G(苏振刚), Yang W-B(杨卫兵), Tian J-C(田纪春), Song G-Z(宋广芝). The relationships between soluble sugar contents of plant and kernel and dry matter accumulation in different high yielding wheat genotypes. Chin Agric Sci Bull (中国农学通报), 2007, 23(9): 307-311 (in Chinese with English abstract)

[18]Shi Y(石玉), Gu S-B(谷淑波), Xu Z-W(于振文), Xu Z-Z(许振柱). Contents of protein components stored in grains and activities of related enzymes in wheat cultivars in different quality types. Acta Agron Sin (作物学报), 2011, 37(11): 2030-2038 (in Chinese with English abstract)

[19]Akbari M, Wenzl P, Caig V, Carlig J, Xia L, Yang S, Uszynski G, Mohler V, Lehmensiek A, Kuchel H, Hayden M J, Howes N, Sharp P, Vaughan P, Rathmell B, Huttner E, Kilian A. Diversity arrays technology (DArT) for high-throughput profiling of the hexaploid wheat genome. Theor Appl Genet, 2006, 113: 1409-1420

[20]Pestsova E, Ganal M W, Röder M S. Isolation and mapping of microsatellite markers speci?c for the D genome of bread wheat. Genome, 2000, 43: 689-697

[21]Lincoln S E, Daly M J, Lander E S. Constructing genetic maps with MAPMAKER/EXP version 3.0, a tutorial and reference manual. In: Whitehead Inst Biomed Res Tech Rep, 3rd Edn. Whitehead Institute for Biomedical Research, Cambridge, 1993. p 97

[22]Voorrips R E. Map Chart: software for the graphical presentation of linkage maps and QTLs. Heredity, 2002, 93: 77-78

[23]Wang D L, Zhu J, Li Z K, Paterson A H. Mapping QTLs with epistatic effects and QTL × environment interactions by mixed linear model approaches. Theor Appl Genet, 1999, 99: 1255-1264

[24]Yang J, Zhu J. Methods for predicting superior genotypes under multiple environments based on QTL effects. Theor Appl Genet, 2005, 110: 1268-1274

[25]McIntosh R A, Devos K M, Dubcovsky J, Rogers W J, Morris C F, Appels R, Anderson O D. Catalogue of gene symbols for wheat: 2005 supplement. [2010-05-15]. http://wheat.pw.usda.gov/ggpages/wgc/2005upd.html

[26]Sourdille P, Singh S, Cadalen T, Brown-Guedira G L, Gay G, Qi L. Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Funct Integr Genomics, 2004, 4: 12-25

[27]Su Z Q, Hao C Y, Wang L F, Dong Y C, Zhang X Y. Identification and development of a functional marker of TaGW2 associated with grain weight in bread wheat (Triticum aestivum L.). Theor Appl Genet, 2011, 122: 211-223

[28]Boyko E V, Gill K S, Mickelson-Young L, Nasuda S, Raupp W J, Ziegle J N. A high density genetic map of Aegilops tauschii, the D genome progenitor of bread wheat. Theor Appl Genet, 1999, 99: 16-26

[29]Zhang D Q, Zhang Z Y, Yang K. Genome-wide search for segregation distortion loci associated with the expression of complex traits in Populus tomentosa. For Stud China, 2007, 9: 1-6

[30]Börner A, Schumann E, Fürste A, Cöster H, Leithold B, Röder M S, Weber W E. Mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat (Triticum aestivum L.). Theor Appl Genet, 2002, 105: 921-936

[31]McCartney C A, Somers D J, Humphreys D G, Lukow O, Ames N, Noll J, Cloutier S, McCallum B D. Mapping quantitative trait loci controlling agronomic traits in the spring wheat cross genome RL4452 × ‘AC Domain’. Genome, 2005, 48: 870-883

[32]Huang X Q, Cloutier S, Lycar L, Radovanovic N, Humphreys D G, Noll J S, Somers D J, Brown P D. Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats (Triticum aestivum L.). Theor Appl Genet, 2006, 113: 753-766

[33]Wang R X, Hai L, Zhang X Y, You G X, Yan C S, Xiao S H. QTL mapping for grain ?lling rate and yield-related traits in RILs of the Chinese winter wheat population Heshangmai × Yu 8679. Theor Appl Genet, 2009, 118: 313-325

[34]Ding A-M(丁安明), Li J(李君), Cui F(崔法), Zhao C-H(赵春华), Ma H-Y(马航运), Wang H-G(王洪刚). QTL mapping for yield related traits using two associated RIL populations of wheat. Acta Agron Sin (作物学报), 2011, 37(9): 1511-1524 (in Chinese with English abstract)

[35]Wang R-X(王瑞霞), Zhang X-Y(张秀英), Wu L(伍玲), Wang R(王瑞), Hai L(海林), You G-X(游光霞), Yan C-S(闫长生), Xiao S-H(肖世和). QTL analysis of grain size and related traits in winter wheat under different ecological environments. Sci Agric Sin (中国农业科学), 2009, 42(2): 398-407 (in Chinese with English abstract)
[1] HU Wen-Jing, LI Dong-Sheng, YI Xin, ZHANG Chun-Mei, ZHANG Yong. Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat [J]. Acta Agronomica Sinica, 2022, 48(6): 1346-1356.
[2] GUO Xing-Yu, LIU Peng-Zhao, WANG Rui, WANG Xiao-Li, LI Jun. Response of winter wheat yield, nitrogen use efficiency and soil nitrogen balance to rainfall types and nitrogen application rate in dryland [J]. Acta Agronomica Sinica, 2022, 48(5): 1262-1272.
[3] YU Chun-Miao, ZHANG Yong, WANG Hao-Rang, YANG Xing-Yong, DONG Quan-Zhong, XUE Hong, ZHANG Ming-Ming, LI Wei-Wei, WANG Lei, HU Kai-Feng, GU Yong-Zhe, QIU Li-Juan. Construction of a high density genetic map between cultivated and semi-wild soybeans and identification of QTLs for plant height [J]. Acta Agronomica Sinica, 2022, 48(5): 1091-1102.
[4] LEI Xin-Hui, WAN Chen-Xi, TAO Jin-Cai, LENG Jia-Jun, WU Yi-Xin, WANG Jia-Le, WANG Peng-Ke, YANG Qing-Hua, FENG Bai-Li, GAO Jin-Feng. Effects of soaking seeds with MT and EBR on germination and seedling growth in buckwheat under salt stress [J]. Acta Agronomica Sinica, 2022, 48(5): 1210-1221.
[5] FU Mei-Yu, XIONG Hong-Chun, ZHOU Chun-Yun, GUO Hui-Jun, XIE Yong-Dun, ZHAO Lin-Shu, GU Jia-Yu, ZHAO Shi-Rong, DING Yu-Ping, XU Yan-Hao, LIU Lu-Xiang. Genetic analysis of wheat dwarf mutant je0098 and molecular mapping of dwarfing gene [J]. Acta Agronomica Sinica, 2022, 48(3): 580-589.
[6] FENG Jian-Chao, XU Bei-Ming, JIANG Xue-Li, HU Hai-Zhou, MA Ying, WANG Chen-Yang, WANG Yong-Hua, MA Dong-Yun. Distribution of phenolic compounds and antioxidant activities in layered grinding wheat flour and the regulation effect of nitrogen fertilizer application [J]. Acta Agronomica Sinica, 2022, 48(3): 704-715.
[7] LIU Yun-Jing, ZHENG Fei-Na, ZHANG Xiu, CHU Jin-Peng, YU Hai-Tao, DAI Xing-Long, HE Ming-Rong. Effects of wide range sowing on grain yield, quality, and nitrogen use of strong gluten wheat [J]. Acta Agronomica Sinica, 2022, 48(3): 716-725.
[8] XU Long-Long, YIN Wen, HU Fa-Long, FAN Hong, FAN Zhi-Long, ZHAO Cai, YU Ai-Zhong, CHAI Qiang. Effect of water and nitrogen reduction on main photosynthetic physiological parameters of film-mulched maize no-tillage rotation wheat [J]. Acta Agronomica Sinica, 2022, 48(2): 437-447.
[9] HUANG Li, CHEN Yu-Ning, LUO Huai-Yong, ZHOU Xiao-Jing, LIU Nian, CHEN Wei-Gang, LEI Yong, LIAO Bo-Shou, JIANG Hui-Fang. Advances of QTL mapping for seed size related traits in peanut [J]. Acta Agronomica Sinica, 2022, 48(2): 280-291.
[10] ZHANG Yan-Bo, WANG Yuan, FENG Gan-Yu, DUAN Hui-Rong, LIU Hai-Ying. QTLs analysis of oil and three main fatty acid contents in cottonseeds [J]. Acta Agronomica Sinica, 2022, 48(2): 380-395.
[11] YAN Yan, ZHANG Yu-Shi, LIU Chu-Rong, REN Dan-Yang, LIU Hong-Run, LIU Xue-Qing, ZHANG Ming-Cai, LI Zhao-Hu. Variety matching and resource use efficiency of the winter wheat-summer maize “double late” cropping system [J]. Acta Agronomica Sinica, 2022, 48(2): 423-436.
[12] WANG Yang-Yang, HE Li, REN De-Chao, DUAN Jian-Zhao, HU Xin, LIU Wan-Dai, GU Tian-Cai, WANG Yong-Hua, FENG Wei. Evaluations of winter wheat late frost damage under different water based on principal component-cluster analysis [J]. Acta Agronomica Sinica, 2022, 48(2): 448-462.
[13] CHEN Xin-Yi, SONG Yu-Hang, ZHANG Meng-Han, LI Xiao-Yan, LI Hua, WANG Yue-Xia, QI Xue-Li. Effects of water deficit on physiology and biochemistry of seedlings of different wheat varieties and the alleviation effect of exogenous application of 5-aminolevulinic acid [J]. Acta Agronomica Sinica, 2022, 48(2): 478-487.
[14] MA Bo-Wen, LI Qing, CAI Jian, ZHOU Qin, HUANG Mei, DAI Ting-Bo, WANG Xiao, JIANG Dong. Physiological mechanisms of pre-anthesis waterlogging priming on waterlogging stress tolerance under post-anthesis in wheat [J]. Acta Agronomica Sinica, 2022, 48(1): 151-164.
[15] MENG Ying, XING Lei-Lei, CAO Xiao-Hong, GUO Guang-Yan, CHAI Jian-Fang, BEI Cai-Li. Cloning of Ta4CL1 and its function in promoting plant growth and lignin deposition in transgenic Arabidopsis plants [J]. Acta Agronomica Sinica, 2022, 48(1): 63-75.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!