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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (09): 1570-1582.doi: 10.3724/SP.J.1006.2012.01570


Genome-Wide Identification, Classification, and Expression of NF-YB Gene Family in Soybean

ZHENG Wei-Jun1,2,XU Zhao-Shi2,*,FENG Zhi-Juan2,LI Lian-Cheng2,CHEN Ming2,CHAI Shou-Cheng1,MA You-Zhi2   

  1. 1 College of Agronomy, Northwest A & F University, Yangling 712100, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Genetics and Breeding of Ministry of Agriculture, Beijing 100081, China
  • Received:2012-02-17 Revised:2012-04-16 Online:2012-09-12 Published:2012-07-03
  • Contact: 徐兆师, E-mail: xuzhaoshi@yahoo.com.cn, Tel: 010-82106773; 柴守诚, E-mail: chaishoucheng@126.com, Tel: 029-87082205

Abstract: Based on soybean genome database and bioinformatics method, we obtained soybean NF-YB family genes and their positions on chromosome and duplication information. NF-YB proteins were classified according to their phylogenetic relationship. The soybean RNA-sequencing data from the SoyBase database and EST data from NCBI UniGene were used to analyze the expression pattern of these genes at different development stages. A total of 28 NF-YB genes were systematically identified from soybean and classified into three types. They were located on 14 chromosomes and most of them had stress related cis-acting elements in their promoter regions. Ten differentially expressed genes were found at each developmental stage, and four of them were highly expressed in different tissues other than root nodules and root. Among other six genes, two were highly expressed in root nodule, two were specifically expressed in root nodules and two were specifically expressed in root. The results facilitate functional analysis and utilization of NF-YB genes in crop genetic improvement.

Key words: NF-YB, Genome-wide identification, Phylogeny, Differential expression, Promoter

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