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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (12): 2104-2117.doi: 10.3724/SP.J.1006.2014.02104

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Construction of DNA Fingerprinting and Analysis of Genetic Diversity for Xinluzao Cotton Varieties

NIE Xin-Hui1,2,**,YOU Chun-Yuan2,**,LI Xiao-Fang3,QIN Jiang-Hong2,HUANG Cong1,GUO Huan-Le1,WANG Xia-Qing1,ZHAO Wen-Xia1LIN Zhong-Xu1,*   

  1. 1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; 2 Cotton Institute, Shihezi Academy of Agricultural Sciences, Xinjiang 832000, China?; 3Changjiang University, Jingzhou 434025, China
  • Received:2014-04-02 Revised:2014-09-16 Online:2014-12-12 Published:2014-10-16
  • Contact: 林忠旭, E-mail: linzhongxu@mail.hzau.edu.cn ** 同等贡献 (Contributed equally to this work)

Abstract:

Fifty-one Xinluzao conventional cotton varieties authorized before 2013 were detected with 75 pairs of primers with high polymorphism, good repeatability, and even distribution on 26 chromosomes (2–3 pairs from each chromosome) selected from 5000 pairs of SSR primers. A total of 226 polymorphic loci were obtained, and each marker detected 2–12 polymorphic loci with an average of 3.01; the polymorphism information content ranged from 0.0799 to 0.8752, with an average of 0.6624. The results showed that 21 varieties could be differentiated by characteristic primers, the rest 30 varieties could be identified by primer conbinations. In the end, 40 characteristic primers and combinated primers could completely differentiate the 51 Xinluzao cotton varieties from each other. DNA fingerprinting of the 51 Xinluzao conventional varieties were constructed with the 40 pairs of SSR markers. NTSYS-pcV2.10 software was used to analyze genetic diversity of the 51 conventional varieties, the results indicated that genetic similarity coefficient for the varieties ranged from 0.4269 to 0.9873, with an average of 0.7071, showing the narrow genetic diversity in Xinluzao cotton varieties. The 51 Xinluzao cotton varieties were divided into four types with the genetic similarity coefficient matrix and cluster analysis, which were strongly consistent with their pedigrees.

Key words: Xinluzao conventional cotton varieties, SSR, DNA fingerprinting, Genetic diversity

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