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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (03): 414-421.doi: 10.3724/SP.J.1006.2015.00414

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Functional Analysis of Small Heat Shock Protein Gene ZmHSP17.7 from Maize

SUN Ai-Qing1,**,GE Shu-Juan2,**,DONG Wei2,SHAN Xiao-Di3,DONG Shu-Ting1,*,ZHANG Jie-Dao2,*   

  1. 1 State Key Laboratory of Crop Biology / Shandong Key Laboratory of Crop Biology / College of Agriculture, Shandong Agricultural University, Tai’an 271018, China; 2 College of Life Sciences, Shandong Agricultural University, Tai’an 271018, China; 3 College of Life Sciences, Anhui University, Hefei 230036, China
  • Received:2014-06-30 Revised:2014-12-19 Online:2015-03-12 Published:2014-12-29
  • Contact: 董树亭, E-mail: stdong@sdau.edu.cn; 张杰道, E-mail: jdzhang@sdau.edu.cn E-mail:stdong@sdau.edu.cn

Abstract:

Heat shock proteins are typical proteins responding to high temperature. Maize germplasm POB21 was used to isolate a small heat-shock protein gene with 477 bp CDS sequence. This gene encodes a protein with 158 amino acids, which contains the typical ACD domain of HSP20 proteins. Isoelectronic point of ZmHSP17.7 is predicted to be 5.36. It encodes a 17.746 kD protein, and so named as ZmHSP17.7. The homologues can be found in diverse plants such as Arabidopsis and rice. According to the phylogenetic and subcellular localization analyses of this protein, it is a member of CI class of small heat shock protein family. Upon northern blotting analysis, gene expression of ZmHSP17.7 was quickly induced by high temperature. Drought stress under 15% PEG did not independently regulate the gene expression, but enhanced the heat-induced gene expression under combined stress of high temperature and drought. Exogenous ABA did not affect gene expression. The overexpression of ZmHSP17.7 gene in seed germination and plant growth of Arabidopsis showed higher tolerance to stresses of high temperature and drought, indicating that this gene may play a certain role in protecting plant from high temperature, drought and combined stress.

Key words: Maize, Small heat-shock protein, High temperature, Stress resistance

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