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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (08): 1201-1211.doi: 10.3724/SP.J.1006.2015.01201


Expression Profiling Analysis between Resistant and Susceptible Cotton Cultivars (Gossypium hirsutum L.) in Response to Fusarium Wilt

HAN Ze-Gang,ZHAO Zeng-Qiang,LI Hui-Hui,ZHANG Xi,LI Xiao-Ling,ZHANG Wei*   

  1. Agricultural College of Shihezi University / Laboratory of Cotton Molecular Breeding, Shihezi 832000, China
  • Received:2014-11-06 Revised:2015-05-04 Online:2015-08-12 Published:2015-05-15


Fusarium Wilt resistant cotton cultivar Zhongmiansuo 12 and susceptible cultivar Xinluzao 7 were used to analyze the gene expression profiling of root tissues at three hours and six hours after the seedlings infection by Fusarium Wilt using Solexa sequencing technology. Compared with no infection, 4447 and 5481 differential genes after three hours and six hours infected by Fusarium Wilt were identified, and compared six hours with three hours, 2559 differential genes were identified in Zhongmiansuo 12; while 8615, 6727 and 2078 was respectively identified in Xinluzao 7. There were 1879 and 500 differential genes in three hours and six hours after infection when the two cultivars were compared. According to the Gene Ontology, these genes were divided into these groups of biological process, cellular component and molecular function; then further subdivided into 48 functional categories. By analyzing the pathways, the most of them were identified in 6 h/0 h after infection between Zhongmiansuo 12 and Xinluzao 7, which were 126 each in the two cultivars; the least of pathways were at 6 h after infection in two cultivars, which was 89 only. However, all the pathways in each comparison group could be classified into 13 categories, such as biosynthesis of other secondary metabolites, glycan biosynthesis and metabolism, environmental adaptation, Immune system. A pathway, named plant-pathogen interaction in the environmental adaptation and immune system category, was involved in 996 differential genes; and the number of up regulated genes was 444 and that of down regulated genes was 552. The most differential genes were in WRKY transcription factor family, the serine/threonine kinase had the medium number of differential genes, while DNA damage-repair/ toleration protein, JAZ1, RAR1, RPM1-interacting protein, S locus specific glycoprotein S6 precursor and caltractin had the least genes. Finally, six expressed genes were randomly relected to ralidate the results by RT-PCR which were consistent with those by Solexa sequencing.

Key words: Gossypium hirsutum, Fusarium wilt, Solexa, Expression profiling

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