Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (08): 1191-1200.doi: 10.3724/SP.J.1006.2015.01191

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Stress-activated Protein Kinase OsSAPK2 Involved in Regulating Resistant Response to Xanthomonas oryzae pv. oryzae in Rice

HU Dan-Dan1,2,ZHANG Fan2,HUANG Li-Yu2,ZHUO Da-Long1,2,ZHANG Fan2,ZHOU Yong-Li2,*,SHI Ying-Yao2,*,LI Zhi-Kang2   

  1. 1 College of Agronomy, Anhui Agricultural University, Hefei 230036, China; 2 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2015-01-08 Revised:2015-05-04 Online:2015-08-12 Published:2015-06-03
  • Contact: 石英尧,E-mail: shiyy123@163.com, Tel: 0551-65786213; 周永力,E-mail: zhouylcaas@126.com E-mail:hudandan_ahau@163.com

Abstract:

Sucrose nonfermenting1-related protein kinase2 (SnRK2), also known as stress-activated protein kinase (OsSAPKs), plays an important role in signal transduction. In this study, we analyzed the structure and function of OsSAPK2 in response to Xanthomonas oryzae pv. oryzae (Xoo) infection. The result suggested that OsSAPK2 is a member of Kulik’s II group like OsSAPK1, OsSAPK3 and located in nucleus and cytoplasm. OsSAPK2 and disease-resistant genes OsLRR1, OsHIR1 were down regulated in OsSAPK2-RNAi transgenic rice, while disease-related gene OsMAPK5 was up regulated. Compared with non-transgenic plants, transgenic plants were more susceptible to Xoo infection. OsSAPK2 could activate itself and interact with several stress-related proteins. These results indicate that OsSAPK2 might be involved in the regulation of resistance response by regulating the expression of OsLRR1, OsHIR1, OsMAPK5 and interacting with stress-related proteins.

Key words: OsSAPK2, Oryza sativa, Bacterial blight, RNAi

[1]Fujita Y, Fujita M, Satoh R, Maruyama K, Parvez M M, Seki M, Hiratsu K, Ohme-Takagi M, Shinozaki K, Yamaguchi-Shinozaki K. AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis. Plant Cell, 2005, 17: 3470–3488



[2]Furuya T, Matsuoka D, Nanmori T. Membrane rigidification functions upstream of the MEKK1-MKK2-MPK4 cascade during cold acclimation in Arabidopsis thaliana. FEBS Lett, 2014, 588: 2025–2030



[3]Kouzai Y, Mochizuki S, Nakajima K, Desaki Y, Hayafune M, Miyazaki H, Yokotani N, Ozawa K, Minami E, Kaku H, Shibuya N, Nishizawa Y. Targeted gene disruption of OsCERK1 reveals its indispensable role in chitin perception and involvement in the peptidoglycan response and immunity in rice. Mol Plant Microbe Interact, 2014, 27: 975–982



[4]王永波, 高世庆, 唐益苗, 刘美英, 郭丽香, 张朝, 赵昌平. 植物蔗糖非发酵-1相关蛋白激酶家族研究进展. 生物技术通报, 2010, (11): 7–18



Wang Y B, Gao S Q, Tang Y M, Liu M Y, Guo L X, Zhang Z, Zhao C P. Advance of the sucrose non-fermenting-1-related protein in kinase family in plants. Biotechnol Bull, 2010, (11): 7–18 (in Chinese with English abstract)



[5]Kulik A, Wawer I, Krzywińska E, Bucholc M, Dobrowolska G. SnRK2 protein kinases-key regulators of plant response to abiotic stresses. OMICS, 2011, 15: 859–872



[6]Kobayashi Y, Yamamoto S, Minami H, Kagay Y, Hattori T. Diferential activation of the rice sucrose nonfermenting1-related protein kinase 2 family by hyperosmotic stress and abscisic acid. Plant Cell, 2004, 16: 1163–1177



[7]Boudsocq M, Barbier-Brygoo H, Laurière C. Identification of nine sucrose nonfermenting1-related protein kinases 2 activated by hyperosmotic and saline stresses in Arabidopsis thaliana. J Biol Chem, 2004, 279: 41758–41766



[8]Li L B, Zhang Y R, Liu K, Zhong F, Fang Z J, Sun Q X, Gao J W. Identification and bioinformatics analysis of SnRK2 and CIPK family genes in sorghum. Agric Sci China, 2010, 9: 19–30



[9]Huai J, Wang M, He J, Zheng J, Dong Z, Lü H, Zhao J, Wang G. Cloning and characterization of the SnRK2 gene family from Zea mays. Plant Cell Rep, 2008, 27: 1861–1868



[10]Kelner A, Pekala I, Kaczanowski S, Muszynska G, Hardie D G, Dobrowolska G. Biochemical characterization of the tobacco 42-kDa protein kinase activated by osmotic stress. Plant Physiol, 2004, 136: 3255–3265.



[11]Anderberg R J, Walker-Simmons M K. Isolation of a wheat cDNA clone for an abscisic acid-inducible transcript with homology to protein kinases. Proc Natl Acad Sci USA, 1992, 89: 10183–10187



[12]Holappa L D, Walker-Simmons M K. The wheat abscisic acid-responsive protein kinase mRNA, PKABA1, is up-regulated by dehydration, cold temperature, and osmotic stress. Plant Physiol, 1995, 108: 1203–1210



[13]Mao X G, Zhang H Y, Tian S J, Chang X P, Jing R L. TaSnRK2.4, an SNF1-type serine/threonine protein kinase of wheat (Triticum aestivum L.), confers enhanced multistress tolerance in Arabidopsis. J Exp Bot, 2010, 61: 683–696



[14]Zhang H Y, Mao X G, Zhang J N, Chang X P, Wang C S, Jing R L. Genetic diversity analysis of abiotic stress response gene TaSnRK2.7-A incommon wheat. Genetica, 2011, 139: 743–753



[15]Monks D E, Aghoram K, Courtney P D, DeWald D B, Dewey R E. Hyperosmotic stress induces the rapid phosphorylation of a soybean phosphatidylinositol transfer protein homolog through activation of the protein kinases SPK1 and SPK2. Plant Cell, 13: 1205–1219



[16]Miko?ajczyk M, Awotunde O S, Muszyńska G, Klessig D F, Dobrowolska G. Osmotic stress induces rapid activation of a salicylic acid-induced protein kinase and a homolog of protein kinase ASK1 in tobacco cells. Plant Cell, 2000, 12: 165–178



[17]Yoon H W, Kim M C, Shin P G, Kim J S, Kim C Y, Lee S Y. Hwang I, Bahk J D, Hong J C, Han C, Cho M J. Differential expression of two functional serine/threonine protein kinases from soybean that have an unusual acidic domain at the carboxy terminus. Mol Gen Genet, 1997, 255: 359–371



[18]Nakashima K, Fujita Y, Kanamori N, Katagiri T, Umezawa T, Kidokoro S, Maruyama K, Yoshida T, Ishiyama K, Kobayashi M, Shinozaki K, Yamaguchi-Shinozaki K. Three Arabidopsis SnRK2 protein kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3, involved in ABA signaling are essential for the control of seed development and dormancy. Plant Cell Physiol, 2009, 50: 1345–1363



[19]Fujita Y, Yoshida T, Yamaguchi-Shinozaki K. Pivotal role of the AREB/ABF-SnRK2 pathway in ABRE-mediated transcription in response to osmotic stress in plants. Physiol Plant, 2013, 147: 15–27



[20]Shao Y, Qin Y, Zou Y J, Ma F W. Genome-wide identification and expression profiling of the SnRK2 gene family in Malus prunifolia. Gene, 2014, 552: 87–97



[21]Mew T M. Current status and future prospects of research on bacterial blight of rice. Annu Rev Phytopathol, 1987, 25: 359–382



[22]NiÑo-Liu D O, Ronald P C, Bogdanove A J. Xanthomonas oryzae pathovars: model pathogens of a model crop. Mol Plant Pathol, 2006, 7: 303–324



[23]Xu M Y, Huang L Y, Zhang F, Zhu L H, Zhou Y L, Li Z K. Genome-wide phylogenetic analysis of stress-activated protein kinase genes in rice (OsSAPKs) and expression profiling in response to Xanthomonas oryzae pv. oryzicola infection. Plant Mol Biol Rep, 2013, 31: 877–885



[24]杨立桃, 赵志辉, 丁嘉羽, 张承妹, 贾军伟, 张大兵. 利用实时荧光定量PCR方法分析转基因水稻外源基因拷贝数. 中国食品卫生杂志, 2005, 17(2): 140–144



Yang L T, Zhao Z H, Ding J Y, Zhang C M, Jia J W, Zhang D J. Estimating copy number of transgenes in transformed rice by real-time quantitative PCR. Chin J Food Hygiene, 2005, 17(2): 140–144



[25]Cronk Q C. Plant evolution and development in a post-genomic context. Nat Rev Genet, 2001, 2: 607–619



[26]Saha J, Chatterjee C, Sengupta A, Gupta K, Gupta B. Genome-wide analysis and evolutionary study of sucrose non-fermenting1-related protein kinase 2 (SnRK2) gene family members in Arabidopsis and Oryza. Comput Biol Chem, 2014, 49: 59–70



[27]Shukla V, Mattoo A K. Sucrose non-fermenting 1-related protein kinase 2 (SnRK2): a family of protein kinases involved in hyperosmotic stress signaling. Physiol Mol Biol Plants, 2008, 14: 91–100



[28]Yoshida T, Fujita Y, Maruyama K, Mogami J, Todaka D, Shinozaki K, Yamaguchi-Shinozaki K. Four Arabidopsis AREB/ABF transcription factors function predominantly in gene expression downstream of SnRK2 kinases in abscisic acid signalling in response to osmotic stress. Plant Cell Environ, 2015, 38: 35–49



[29]Feng C Z, Chen Y, Wang C, Kong Y H, Wu W H, Chen Y F. Arabidopsis RAV1 transcription factor, phosphorylated by SnRK2 kinases, regulates the expression of ABI3, ABI4, and ABI5 during seed germination and early seedling development. Plant J, 2014, 80: 654–668



[30]Brutus A, Sicilia F, Macone A, Cervone F, De Lorenzo G. A domain swap approach reveals a role of the plant wall-associated kinase1 (WAK1) as a receptor of oligogalacturonides. Proc Natl Acad Sci USA, 2010, 107: 9452–9457



[31]Zhou L, Cheung M Y, Zhang Q, Lei C L, Zhang S H, Sun S M, Lam H M. A novel simple extracellular leucine-rich repeat (eLRR) domain protein from rice (OsLRR1) enters the endosomal pathway and interacts with the hypersensitive-induced reaction protein 1 (OsHIR1). Plant Cell Environ, 2009, 32: 1804–1820



[32]Zhou L, Cheung M Y, Li M W, Fu Y, Sun Z, Sun S M, Lam H M. Rice hypersensitive induced reaction protein1 (OsHIR1) associates with plasma membrane and triggers hypersensitive cell death. BMC Plant Biol, 2010, 10: 290



[33]Xiong L Z, Yang Y N. Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase. Plant Cell, 2003, 15: 745–759



[34]Serra T S, Figueiredo D D, Cordeiro A M, Almeida D M, Lourenço T, Abreu I A, Sebastián A, Fernandes L, Contreras-Moreira B, Oliveira M M, Saibo N J. OsRMC, a negative regulator of salt stress response in rice, is regulated by two AP2/ERF transcription factors. Plant Mol Biol, 2013, 82: 439–455



[35]Nakano T, Suzuki K, Fujimura T, Shinshi H. Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol, 2006, 140: 411–432



[36]Cheong Y H, Moon B C, Kim J K, Kim C Y, Kim M C, Kim I H, Park C Y, Kim J C, Park B O, Koo S C, Yoon H W, Chung W S, Lim C O, Lee S Y, Cho M J. BWMK1, a rice mitogen-activated protein kinase, locates in the nucleus and mediates pathogenesis-related gene expression by activation of a transcription factor. Plant Physiol, 2003, 132: 1961–1972
[1] ZHAO Hai-Han, LIAN Wang-Min, ZHAN Xiao-Deng, XU Hai-Ming, ZHANG Ying-Xin, CHENG Shi-Hua, LOU Xiang-Yang, CAO Li-Yong, HONG Yong-Bo. Genetic dissection of the bacterial blight disease resistance in super hybrid rice RILs using genome-wide association study [J]. Acta Agronomica Sinica, 2022, 48(1): 121-137.
[2] MA Huan-Huan, FANG Qi-Di, DING Yuan-Hao, CHI Hua-Bin, ZHANG Xian-Long, MIN Ling. GhMADS7 positively regulates petal development in cotton [J]. Acta Agronomica Sinica, 2021, 47(5): 814-826.
[3] ZHENG Kai-Li, JI Zhi-Yuan, HAO Wei, TANG Yong-Chao, WEI Ye-Na, HU Yun-Gao, ZHAO Kai-Jun, WANG Chun-Lian. Molecular identification of rice bacterial blight susceptible gene Xig1 and creation of disease resistant resources [J]. Acta Agronomica Sinica, 2020, 46(9): 1332-1339.
[4] XU Xue-Zhong,WANG Ting,WAN Wang,LI Si-Hui,ZHU Guo-Hui*. ABA Biosynthesis Gene OsNCED3 Confers Drought Stress Tolerance in Rice [J]. Acta Agron Sin, 2018, 44(01): 24-31.
[5] ZHANG Xiao-Qiong, WANG Xiao-Wen, TIAN Wei-Jiang, ZHANG Xiao-Bo, Sun Ying, LI Yang-Yang, Xie Jia, HE Guang-Hua,SANG Xian-Chun. LAZY1 Regulates the Development of Rice Leaf Angle through BR Pathway [J]. Acta Agron Sin, 2017, 43(12): 1767-1773.
[6] ZHONG Jie,WEN Pei-Zheng,SUN Zhi-Guang,XIAO Shi-Zhuo,HU Jin-Long,ZHANG Le,JIANG Ling,CHENG Xia-Nian,LIU Yu-Qiang,WAN Jian-Min. Identification of QTLs Conferring Small Brown Planthopper Resistance in Rice (Oryza sativa L.) Using MR1523/Suyunuo F2:3 Population [J]. Acta Agron Sin, 2017, 43(11): 1596-1602.
[7] SHEN Ya-Lin,ZHUANG Hui,CHEN Huan,ZENG Xiao-Qin,LI Xiang-Ning,ZHANG Jun,ZHENG Hao, LING Ying-Hua,LI Yun-Feng*. Characterization and Gene Mapping of sostenuto floret opening 1 (sfo1) Mutant in Rice (Oryza sativaL.) [J]. Acta Agron Sin, 2017, 43(08): 1122-1127.
[8] ZHOU Ke,LI Yan,WANG Shi-Ming,CUI Guo-Qing,YANG Zheng-Lin,HE Guang-Hua,LING Ying-Hua,ZHAO Fang-Ming. Identification of Rice Chromosome Segment Substitution Line Z519 with Purple Sheath and Candidate Gene Analysis of PSH1 [J]. Acta Agron Sin, 2017, 43(07): 974-982.
[9] XIAO Yan-Hua**, CHEN Xin-Long**, DU Dan, XING Ya-Di, ZHANG Tian-Quan, ZHU Mao-Di,LIU Ming-Ming,ZHU Xiao-Yan, SANG Xian-Chun,HE Guang-Hua*. Identification and Gene Mapping of Starch Accumulation and Early Senescence Leaf Mutant esl9 in Rice [J]. Acta Agron Sin, 2017, 43(04): 473-482.
[10] WANG Sha,HE Yong,LUO Guang-Yu,YAO Min,ZHANG Xu,CHEN Xin-Bo,ZHOU Xiao-Yun. Influence of OsWR2-RNAi on Rice Cuticle Biosynthesis and Drought Resistance [J]. Acta Agron Sin, 2017, 43(03): 315-323.
[11] LI Chuang, LIU Cheng-Chen, ZHANG Chang-Quan, ZHU Ji-Hui, XU Xiao-Ying, ZHAO Fu-Wei,HUANG Shao-Wen, JIN Yin-Gen,LIU Qiao-Quan. Genetic Diversity of ALK Gene and Its Association with Grain Gelatinization Temperature in Currently Cultivated Rice Landraces from Hani’s Terraced Fields in Yunnan Province [J]. Acta Agron Sin, 2017, 43(03): 343-353.
[12] ZHU Ji-Feng,WU Jing,WANG Lan-Fen,ZHU Zhen-Dong,WANG Shu-Min. Mapping of Common Bacterial Blight Resistance Gene in Common Bean [J]. Acta Agron Sin, 2017, 43(01): 1-8.
[13] YANG Xiang-Dong,NIU Lu,ZHANG Wei,YANG Jing,DU Qian,XING Guo-Jie,GUO Dong-Quan,LI Qi-Yun,DONG Ying-Shan. RNAi-mediated SMV-P3 Silencing Increases Soybean Resistance to Soybean Mosaic Virus [J]. Acta Agron Sin, 2016, 42(11): 1647-1655.
[14] GUO Dan,SHI Yong-Feng,WANG Hui-Mei,ZHANG Xiao-Bo,SONG Li-Xin,XU Xia,HE Yan,GUO Liang,WU Jian-Li*. Characterization and Gene Fine Mapping of a Rice Dominant Spotted-leaf Mutant [J]. Acta Agron Sin, 2016, 42(07): 966-975.
[15] YANG Bo,XIA Min, ZHANG Xiao-Bo,WANG Xiao-Wen,ZHU Xiao-Yan,HE Pei-Long,HE Guang-Hua,SANG Xian-Chun*. Identification and Gene Mapping of an Early Senescent Leaf Mutant esl6 in Oryza sativa L. [J]. Acta Agron Sin, 2016, 42(07): 976-983.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!