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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (07): 966-975.doi: 10.3724/SP.J.1006.2016.00966


Characterization and Gene Fine Mapping of a Rice Dominant Spotted-leaf Mutant

GUO Dan,SHI Yong-Feng,WANG Hui-Mei,ZHANG Xiao-Bo,SONG Li-Xin,XU Xia,HE Yan,GUO Liang,WU Jian-Li*   

  1. State Key Laboratory of Rice Biology / Chinese National Center for Rice Improvement / China National Rice Research Institute, Hangzhou 310006, China?
  • Received:2015-12-29 Revised:2016-05-09 Online:2016-07-12 Published:2016-05-11
  • Contact: 吴建利, E-mail: beishangd@163.com E-mail:1409882837@qq.com
  • Supported by:

    This study was supported by the Natural Science Foundation of Zhejiang Province (LQ15C130005) and the National High-tech R&D Program of China (2014AA10A603-15).


A stable inherited rice spotted-leaf mutant HM113 was isolated from an EMS-induced IR64 mutant bank. Under natural conditions, brown lesions were observed on the leaves in three weeks after sowing and spread to the sheaths at the initial heading stage. Agronomic traits including the plant height, panicle length, number of panicles, number of filled grain/panicle, seed-setting rate and 1000-grain weight were decreased significantly in HM113. In addition, the photosynthetic pigment contents, net photosynthetic rate and soluble protein content in the mutant were significantly lower than those in the wild type IR64, while the MDA content was similar to that in the wild-type. Activities of CAT and SOD were significantly lower and activity of POD was significantly higher in the mutant than in IR64. Histochemical analysis showed that cell death and ROS accumulation were occurred in and around the lesions in HM113. Furthermore, disease resistance to bacterial blight pathogens was significantly enhanced in the mutant in contrast to that in the wild type IR64. Expression of defense-related genes including AOS2, PAL4, PR10,and PR1b was apparently up-regulated in the mutant. Genetic analysis indicated that the mutant trait was controlled by a novel single dominant nuclear gene, tentatively termedas SplHM113, which was detected to be located in a region around 308 kb flanked by RM21605 and RM418 on the long arm of chromosome 7. The data and populations obtained in the present study would facilitate the isolation and functional analysis of SplHM113.

Key words: Rice, Spotted-leaf mutant, Bacterial blight resistance, Reactive oxygen species, Gene mapping

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