Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (07): 966-975.doi: 10.3724/SP.J.1006.2016.00966

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Characterization and Gene Fine Mapping of a Rice Dominant Spotted-leaf Mutant

GUO Dan,SHI Yong-Feng,WANG Hui-Mei,ZHANG Xiao-Bo,SONG Li-Xin,XU Xia,HE Yan,GUO Liang,WU Jian-Li*   

  1. State Key Laboratory of Rice Biology / Chinese National Center for Rice Improvement / China National Rice Research Institute, Hangzhou 310006, China?
  • Received:2015-12-29 Revised:2016-05-09 Online:2016-07-12 Published:2016-05-11
  • Contact: 吴建利, E-mail: beishangd@163.com E-mail:1409882837@qq.com
  • Supported by:

    This study was supported by the Natural Science Foundation of Zhejiang Province (LQ15C130005) and the National High-tech R&D Program of China (2014AA10A603-15).

Abstract:

A stable inherited rice spotted-leaf mutant HM113 was isolated from an EMS-induced IR64 mutant bank. Under natural conditions, brown lesions were observed on the leaves in three weeks after sowing and spread to the sheaths at the initial heading stage. Agronomic traits including the plant height, panicle length, number of panicles, number of filled grain/panicle, seed-setting rate and 1000-grain weight were decreased significantly in HM113. In addition, the photosynthetic pigment contents, net photosynthetic rate and soluble protein content in the mutant were significantly lower than those in the wild type IR64, while the MDA content was similar to that in the wild-type. Activities of CAT and SOD were significantly lower and activity of POD was significantly higher in the mutant than in IR64. Histochemical analysis showed that cell death and ROS accumulation were occurred in and around the lesions in HM113. Furthermore, disease resistance to bacterial blight pathogens was significantly enhanced in the mutant in contrast to that in the wild type IR64. Expression of defense-related genes including AOS2, PAL4, PR10,and PR1b was apparently up-regulated in the mutant. Genetic analysis indicated that the mutant trait was controlled by a novel single dominant nuclear gene, tentatively termedas SplHM113, which was detected to be located in a region around 308 kb flanked by RM21605 and RM418 on the long arm of chromosome 7. The data and populations obtained in the present study would facilitate the isolation and functional analysis of SplHM113.

Key words: Rice, Spotted-leaf mutant, Bacterial blight resistance, Reactive oxygen species, Gene mapping

[1]Heath M C. Hypersensitive response-related death. Plant Mol Biol, 2000, 44: 321–334
[2]夏启中, 吴家和, 张献龙. 与植物超敏反应(HR)相关的细胞编程性死亡. 华中农业大学学报, 2005, 24: 97–103
Xia Q Z, Wu J H, Zhang X L. Review on hypersensitive response-related PCD in plant. J Huazhong Agric Univ, 2005, 24: 97–103 (in Chinese with English abstract)
[3]Durrant W E, Dong X. Systemic acquired resistance. Annu Rev Phytopathol, 2004, 42: 185–209
[4]Hu G, Yalpani N, Briggs S P, Johal G S. A porphyrin pathway impairment is responsible for the phenotype of a dominant disease lesion mimic mutant of maize. Plant Cell, 1998, 10: 1095–1105
[5]Balague C, Lin B, Alcon C, Flottes G, Malmstrom S, Kohler C, Neuhaus G, Pelletier G, Gaymard F, Roby D. HLM1, an essential signaling component in the hypersensitive response, is a member of the cyclic nucleotide-gated channel ion channel family. Plant Cell, 2003, 15: 365–379
[6]Feng B H, Yang Y, Shi Y F, Shen H C, Wang H M, Huang Q N, Xu X, Lv X G, Wu J L. Characterization and genetic analysis of a novel rice spotted-leaf mutant HM47 with broad-spectrum resistance to Xanthomonas oryzae pv. oryzae. J Integr Plant Biol, 2013, 55: 473–483
[7]Shen H C, Shi Y F, Feng B H, Wang H M, Xu X, Huang Q N, Lv X G, Wu J L. Identification and genetic analysis of a novel rice spotted-leaf mutant with broad-spectrum resistance to Xanthomonas oryzae pv. oryzae. J Integr Agric, 2014, 13: 713–721
[8]李小红, 施勇烽, 张晓波, 奉保华, 宋莉欣, 王惠梅, 徐霞, 黄奇娜, 郭丹, 吴建利. 水稻斑点叶突变体hm197的鉴定及其基因定位. 中国水稻科学, 2015, 29: 447–456
Li X H, Shi Y F, Zhang X B, Feng B H, Song L X, Wang H M, Xu X, Huang Q N, Guo D, Wu J L. Identification and gene mapping of a spotted leaf mutant hm197 in rice. Chin J Rice Sci, 2015, 29: 447–456 (in Chinese with English abstract)
[9]Jung Y H, Lee J H, Agrawal G K, Rakwal R, Kim J A, Shim J K, Lee S K, Jeon J S, Koh H J, Lee Y H, Iwahashi H, Jwa N S. The rice (Oryza sativa) blast lesion mimic mutant, blm, may confer resistance to blast pathogens by triggering multiple defense-associated signaling pathways. Plant Physiol Biochem, 2005, 43: 397–406
[10]Wu C, Bordeos A, Madamba M R, Baraoidan M, Ramos M, Wang G L, Leach J E, Leung H. Rice lesion mimic mutants with enhanced resistance to diseases. Mol Genet Genomics, 2008, 279: 605–619
[11]Fujiwara T, Maisonneuve S, Isshiki M, Mizutani M, Chen L, Wong H L, Kawasaki T, Shimamoto K. Sekiguchi lesion gene encodes a cytochrome P450 monooxygenase that catalyzes conversion of tryptamine to serotonin in rice. J Biol Chem, 2010, 285: 11308–11313
[12]Wang L, Pei Z, Tian Y, He C. OsLSD1, a rice zinc finger protein, regulates programmed cell death and callus differentiation. Mol Plant Microbe Interact, 2005, 18: 375–384
[13]Sun C, Liu L, Tang J, Lin A, Zhang F, Fang J, Zhang G, Chu C. RLIN1, encoding a putative coproporphyrinogen III oxidase, is involved in lesion initiation in rice. J Genet Genomics, 2011, 38: 29–37
[14]Sakuraba Y, Rahman M L, Cho S H, Kim Y S, Koh H J, Yoo S C, Paek N C. The rice faded green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions. Plant J, 2013, 74: 122–133
[15]Yamanouchi U, Yano M, Lin H, Ashikari M, Yamada K. A rice spotted leaf gene, Spl7, encodes a heat stress transcription factor protein. Proc Natl Acad Sci USA, 2002, 99: 7530–7535
[16]Jiang C J, Shimono M, Maeda S, Inoue H, Mori M, Hasegawa M, Sugano S, Takatsuji H. Suppression of the rice fatty-acid desaturase gene OsSSI2 enhances resistance to blast and leaf blight diseases in rice. Mol Plant Microbe Interact, 2009, 22: 820–829
[17]Qiao Y, Jiang W, Lee J, Park B, Choi M S, Piao R, Woo M O, Roh J H, Han L, Paek N C, Seo H S, Koh H J. SPL28 encodes a clathrin-associated adaptor protein complex 1, medium subunit micro 1 (AP1M1) and is responsible for spotted leaf and early senescence in rice (Oryza sativa). New Phytol, 2010, 185: 258–274
[18]Fekih R, Tamiru M, Kanzaki H, Abe A, Yoshida K, Kanzaki E, Saitoh H, Takagi H, Natsume S, Undan J R, Undan J, Terauchi R. The rice (Oryza sativa L.) LESION MIMIC RESEMBLING, which encodes an AAA-type ATPase, is implicated in defense response. Mol Genet Genomics, 2015, 290: 611–622
[19]Arnon D I. Copper enzymes in isolated chloroplasts polyphenoloxidase in Beta vulgaris. Plant Physiol, 1949, 24: 1–15
[20]Wellburn A R. The spectral determination of chlorophyll a and b, as well as total carotenoids, using various solvents with spectro-photometers of different resolution. Plant Physiol, 2015, 144: 307–313
[21]赵世杰, 史国安, 董新纯. 植物生理学实验指导. 北京: 中国农业科学技术出版社, 2002. pp 134–143
Zhao S J, Shi G A, Dong X C. Plant Physiology Experiment Instruction. Beijing: China Agricultural Science and Technology Press, 2002. pp 134–143 (in Chinese)
[22]Huang Q N, Shi Y F, Zhang X B, Song L X, Feng B H, Wang H M, Xu X, Li X H, Guo D, Wu J L. Single base substitution in OsCDC48 is responsible for premature senescence and death phenotype in rice. J Integr Plant Biol, 2016, 58: 12–28
[23]Yin Z, Chen J, Zeng L, Goh M, Leung H, Khush G S, Wang G L. Characterizing rice lesion mimic mutants and identifying a mutant with broad-spectrum resistance to rice blast and bacterial blight. Mol Plant Microbe Interact, 2000, 13: 869–876
[24]Kauffman H E, Reddy A P D, Ksiek S P V, Marca S D. An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Dis Rep, 1973, 57: 537–541
[25]卢扬江, 郑康乐. 提取水稻DNA的一种简易方法. 中国水稻科学, 1992, 6: 47–48
Lu Y J, Zheng K L. A simple method for isolation of rice DNA. Chin J Rice Sci, 1992, 6: 47–48 (in Chinese with English abstract)
[26]Shi Y F, Chen J, Liu W Q, Huang Q N, Shen B, Leung H, Wu J L. Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.). Sci China Ser C-Life Sci, 2009, 52: 885–890
[27]代高猛, 朱小燕, 李云峰, 凌英华, 赵芳明, 杨正林, 何光华. 水稻类病斑突变体spl31的遗传分析与基因定位. 作物学报, 2013, 39: 1223–1230
Dai G M, Zhu X Y, Li Y F, Ling Y H, Zhao F M, Yang Z L, He G H. Genetic analysis and fine mapping of a lesion mimic mutant spl31 in rice. Acta Agron Sin, 2013, 39: 1223–1230 (in Chinese with English abstract)
[28]宋莉欣, 黄奇娜, 奉保华, 施勇烽, 张晓波, 徐霞, 王惠梅, 李小红, 赵宝华, 吴建利. 水稻斑点叶突变体spl21的鉴定与基因定位. 作物学报, 2015, 41: 1519–1528
Song L X, Huang Q N, Feng B H, Shi Y F, Zhang X B, Xu X, Wang H M, Li X H, Zhao B H, Wu J L. Characterization and gene mapping of a spotted-leaf mutant spl21 in rice (Oryza sativa L.). Acta Agron Sin, 2015, 41: 1519–1528 (in Chinese with English abstract)
[29]Xiao G Q, Zhang H W, Lu X Y, Huang R F. Characterization and mapping of a novel light-dependent lesion mimic mutant lmm6 in rice (Oryza sativa L.). J Integr Agric, 2015, 14: 1687–1696
[30]Jacks Thomas J, Davidonis Gayle H. Superoxide, hydrogen peroxide, and the respiratory burst of fungally infected plant cells. Mol Cellular Biochem, 1996, 158: 77–79
[31]Asada K. Ascorbate peroxidase-a hydrogen peroxide-scavenging enzyme in plants. Physiol Plant, 1992, 85: 235–241
[32]Minibayeva F, Beckett R P, Kranner I. Roles of apoplastic peroxidases in plant response to wounding. Phytochemistry, 2015, 112: 122–129
[33]Kawano T. Roles of the reactive oxygen species-generating peroxidase reactions in plant defense and growth induction. Plant Cell Rep, 2003, 21: 829–837
[34]Whitaker C, Beckett R P, Minibayevab F V, Kranner I. Production of reactive oxygen species in excised, desiccated and cryopreserved explants of Trichilia dregeana Sond. S Afr J Bot, 2010, 76: 112–118
[35]金杨. 水稻类病变突变体spl5细胞坏死机制及其抗病性的研究. 浙江师范大学硕士学位论文, 浙江金华, 2009
Jin Y. Mechanisms of Cell Death and Its Resistance in Rice Lesion Mimic Mutant spl5. MS Theises of Zhejiang Normal University, Jinhua, China, 2009 (in Chinese with English abstract)
[36]Kaurilind E, Xu E, Brosche M. A genetic framework for H2O2 induced cell death in Arabidopsis thaliana. BMC Genomics, 2015, 16: 837–853
[37]Kariola T, Brader G, Li J, Palva E T. Chlorophyllase 1, a damage control enzyme, affects the balance between defense pathways in plants. Plant Cell, 2005, 17: 282–294
[38]Chern M, Fitzgerald H A, Canlas P E, Navarre D A, Ronald P C. Overexpression of a rice NPR1 homolog leads to constitutive activation of defense response and hypersensitivity to light. Mol Plant Microbe Interact, 2005, 18: 511–520
[39]Mori M, Tomita C, Sugimoto K, Hasegawa M, Hayashi N, Dubouzet J G, Ochiai H, Sekimoto H, Hirochika H, Kikuchi S. Isolation and molecular characterization of a Spotted leaf 18 mutant by modified activation-tagging in rice. Plant Mol Biol, 2007, 63: 847–860
[40]钟振泉, 罗文龙, 刘永柱, 王慧, 陈志强, 郭涛. 一份新的水稻斑点叶突变体spl32的鉴定和基因定位. 作物学报, 2015, 41: 861–871
Zhong Z Q, Luo W L, Liu Y Z, Wang H, Chen Z Q, Guo T. Characterization of a novel spotted leaf mutant spl32 and mapping of Spl32(t) gene in rice (Oryza sativa). Acta Agron Sin, 2015, 41: 861–871 (in Chinese with English abstract)
[41]Hwang S H, Hwang D J. Isolation and characterization of the rice NPR1 promoter. Plant Biotechnol Rep, 2010, 4: 29–35
[42]Yuan Y X, Zhong S H, Li Q, Zhu Z R, Lou Y G, Wang L Y, Wang J J, Wang M Y, Li Q L, Yang D L, He Z H. Functional analysis of rice NPR1-like genes reveals that OsNPR1/NHI is the rice orthologue conferring disease resistance with enhanced herbivore susceptibility. Plant Biotechnol J, 2007, 5: 313–324
[43]Li Z, Zhang Y X, Liu L, Liu Q E, Bi Z Z, Yu N, Cheng S H, Cao L Y. Fine mapping of the lesion mimic and early senescence 1 (lmes1) in rice (Oryza sativa). Plant Physiol Biochem, 2014, 80: 300–307
[44]Chen X F, Pan J W, Cheng J, Jiang G H, Jin Y, Gu Z M, Qian Q, Zhai W X, Ma B J. Fine genetic mapping and physical delimitation of the lesion mimic gene spotted leaf 5 (spl5) in rice (Oryza sativa L.). Mol Breed, 2009, 24: 387–395
[45]Babu R, Jiang C J, Xu X, Kottapalli K R, Takatsuji H, Miyao A, Hirochika H, Kawasaki S. Isolation, fine mapping and expression profiling of a lesion mimic genotype, spl(NF4050-8) that confers blast resistance in rice. Theor Appl Genet, 2011, 122: 831–854
 
[1] Hu Zhao, Qian Run, Xie Feng-Pu, Ying Su-Ping. Genome-wide identification and expression analysis of the SPX gene family in rice under phosphorus treatment [J]. Acta Agronomica Sinica, 2026, 52(6): 1902-1912.
[2] Zou Yi-Mei, Xu Min, Wang Hai-Yang, Yao Hui, Wang Jia-Feng, Liu Hao, Ren Dai-Sheng. Analysis of transcription factor regulatory networks in two-line male sterile rice seedling roots in response to salt stress [J]. Acta Agronomica Sinica, 2026, 52(6): 1728-1742.
[3] Yan An, Jiang Kun-Wei, Wang Rong-Yuan, Tian Lin, Zhang Lu, Wang Yun, Xu Jian-Long. Identification and cloning of SVN7 controlling small vascular bundle number in the rice flag leaf [J]. Acta Agronomica Sinica, 2026, 52(5): 1364-1372.
[4] Chen Wei, Wei Wan-Juan, Zhao Qi-Bing, Chang Dong-Wei, Yu Ling-Bo, Zhai Peng-Fei, Feng Zhi-Ming, Chen Zong-Xiang, Ren Yang-Tao, Yang Peng, Liu Hai-Lang, Li Zhen-Fu, Yang Yong-Le, Jin Yan-Gang, Zuo Shi-Min. Developing new germplasm of high-quality and early-maturing rice by editing Hd6 via CRISPR/Cas9 [J]. Acta Agronomica Sinica, 2026, 52(4): 1046-1056.
[5] Shi Shao-Jie, Liu Kai, Chen Zi-Yi, Wang Hui-Ying, Li San-He, Zhou Lei, You Ai-Qing. Cloning and functional analysis of the dwarf and multi-tiller gene DMT1 in rice [J]. Acta Agronomica Sinica, 2026, 52(4): 1022-1034.
[6] Liu Chang-You, Wang Shen, Shi Hui-Ying, Shen Ying-Chao, Sun Lei, Wang Yan, Zhang Zhi-Xiao, Su Qiu-Zhu, Tian Jing, Fan Bao-Jie. QTL mapping for bruchid resistance in an adzuki bean distant hybridization population using rice bean genetic resources [J]. Acta Agronomica Sinica, 2026, 52(3): 936-944.
[7] Ye Fan, Li Shuai, Li Si-Yu, Chen Yun, Dou Chao-Yin, Liu Li-Jun. Effects of water-saving irrigation on rice yield and population quality in Northeast China [J]. Acta Agronomica Sinica, 2026, 52(3): 895-907.
[8] Liu Ning, Fan Ping, Wang Cheng, Chen Qi-Qi, Cheng Qing-Yue, Tie Xia-Na, Tang Jing-Sha, Liu Bin-Bin, Xie Hong-Kun, Wang Jia-Yue, Shi Yuan-Qing, Ma Jun. Effects of reduced nitrogen application combined with organic fertilizer on yield formation and nitrogen utilization in mechanically transplanted rice [J]. Acta Agronomica Sinica, 2026, 52(3): 866-880.
[9] Qin Yi-Yan, Fu Yao, Su Chang, Li Na, Xu Jing-Ru, Cheng Xiao-Ran, Zhang Qi, Zhao Ming-Hui. Functional analysis of OsST41 regulating salt tolerance in rice seedlings [J]. Acta Agronomica Sinica, 2026, 52(3): 802-812.
[10] Zhu Jin-Juan, Wang Hui-Ping, Yang Guo-Dong, Wang Yu-Cheng, Yang Chen, Wang Bin, Agustiani Nurwulan, Tu Jun-Ming, Bi Jun-Guo, Cui Ke-Hui, Huang Jian-Liang, Peng Shao-Bing, Yuan Shen. Effects of water management and variety type on grain yield and quality in ratoon rice [J]. Acta Agronomica Sinica, 2026, 52(1): 295-315.
[11] WANG Chan, WU Ying-Ying, LI Wen-Qi, LI Xia, WANG Fang-Quan, ZHOU Tong, YANG Jie. Development of functional markers of rice stripe disease resistance gene STV11 based on HRM technique [J]. Acta Agronomica Sinica, 2025, 51(9): 2547-2556.
[12] GUO Bao-Wei, WANG Wang, WANG Kai, WANG Yan, ZENG Xin, JING Xiu, WANG Jing, NI Xin-Hua, XU Ke, ZHANG Hong-Cheng. Population dynamic characteristics and formation mechanisms of super high-yielding of two types of glutinous rice in the middle and lower reaches of the Yangtze Rive [J]. Acta Agronomica Sinica, 2025, 51(9): 2433-2453.
[13] CHEN Hui-Ying, HE Jia-Xin, ZHU Bin, HUANG Shi-Xuan, ZHOU Xing-You, WU Jun-Quan, YANG Mei-Yan. Whole genome analysis and biological characterization of phage vB_XaS_ HDB2 infected with Xanthomonas oryzae pv. oryzae [J]. Acta Agronomica Sinica, 2025, 51(8): 2087-2099.
[14] YANG Hai-Yang, WU Lin-Xuan, LI Bo-Wen, SHI Han-Feng, YUAN Xi-Long, LIU Jin-Zhao, CAI Hai-Rong, CHEN Shi-Yi, GUO Tao, WANG Hui. OsWRI3, identified based on QTL mapping, regulates seed shattering in rice [J]. Acta Agronomica Sinica, 2025, 51(7): 1712-1724.
[15] WANG Fen, WU Dong-Li, ZHANG Quan-Jun. Response of phenological phase stages of single-cropping rice to climate change in Hubei province, China [J]. Acta Agronomica Sinica, 2025, 51(7): 1934-1948.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!