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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (01): 1-8.doi: 10.3724/SP.J.1006.2017.00001


Mapping of Common Bacterial Blight Resistance Gene in Common Bean

ZHU Ji-Feng,WU Jing,WANG Lan-Fen,ZHU Zhen-Dong,WANG Shu-Min   

  1. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2016-05-04 Revised:2016-07-11 Online:2017-01-12 Published:2016-07-28
  • Contact: 王述民. E-mail: wangshumin@caas.cn E-mail:zhujifeng0224@163.com
  • Supported by:

    This study was supported by the China Agriculture Research System (CARS-09), the National Key Technology R&D Program of China (2013BAD01B03-18a), and the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences.


Common bacterial blight disease is a serious disease affecting the production of common bean (Phaseolus vulgaris L.) worldwide. The common bean germplasm Longyundou 5 is the main cultivar carrying common bacterial blight resistance genein Heilongjiang province. To study the genetic mechanisms behind this resistance, we constructed 785 F2 plants from Longyundou 5. Linkage analysis was performed on this population by using SSR markers. A linkage map covering 1648.42 cM with an average marker distance of 8.00 cM among 206 SSR markers was constructed. This map contained 12 linkage groups with a mean group length of 137.37 cM and the number of loci ranging from three to thirty-five. Combined with the results of phenotypic evaluation, QTL analysis was performed by the inclusive composite interval mapping method with QTL IciMapping v4.0. A QTL was detected between p6s249 and p6s183 on chromosome Pv06, with an additive effect of 0.44, which means the favourable gene of this locus is from Longyundou 5. The LOD score of the QTL was 5.93 and the total phenotypic variation at 14 days after inoculation was 4.61%. These results showed the effect of the QTL was low, which will play an important role in breeding durable resistant varieties of common bean. Association analysis between 11 SSR primers linked with common bacterial blight resistance and the disease rating showed that SSR marker p6s249 is excellently associated (P<0.001) with common bacterial blight resistance and can be used for marker assisted selection.

Key words: Phaseolus vulgaris L, Common bacterial blight, SSR, QTL, Association analysis

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