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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (08): 1122-1127.doi: 10.3724/SP.J.1006.2017.01122


Characterization and Gene Mapping of sostenuto floret opening 1 (sfo1) Mutant in Rice (Oryza sativaL.)

SHEN Ya-Lin,ZHUANG Hui,CHEN Huan,ZENG Xiao-Qin,LI Xiang-Ning,ZHANG Jun,ZHENG Hao, LING Ying-Hua,LI Yun-Feng*   

  1. Rice Research Institute, Southwest University/Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops,Chongqing 400716, China
  • Received:2016-12-01 Revised:2017-04-20 Online:2017-08-12 Published:2017-04-27
  • Contact: Li Yuefeng, E-mail: liyf1980@swu.edu.cn
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31271304) and Fundamental Research Funds for the Central Universities (XDJK2016A013).


Rice floret opening is one of the most critical physiological processes in its reproductive development, which has a significant influence on the fertilization and subsequent seed development. Rice floret opening and closing are promoted respectively by the lodicules’ expansion and shrink. In recent years, many studies focused on the molecular mechanism of rice lodiculedevelopment.However, few studies focused on the molecular regulation mechanism in physiological process of lodicule opening and closing. In this paper, we reported a floret opening mutant, sostenuto floret opening 1 (sfo1), derived from EMS (ethyl methane sulfonate) mutation groups of Xinong 1B.During the floret opening stage, the florets of sfo1 and wild type were observed directly by both the stereoscope and the scanning electron microscopy at about 1 h before opening, and 10 min, 1.5 h and 48 h after openingrespecting. Compared withthat in the wild type, the sfo1floret showed a delay of lodicules dehydration after floret opening, resulting in its failing to close the lemma and palea. Significant differenceswere not found at1h before opening, showing closed hulls, flat lodicules and well-stackedlodicules surface cells.At 10 min after opening, there were also no obvious differences between wild type and mutants,showing openinghulls, inflated lodicules, and smooth and well-stacked surface cells. At1.5 h after opening, sfo1 hulls were not closed so big as those in the wild type. And sfo1lodicules still inflated, but not dehydrated and atrophied as those in thewild type. At 48 h after opening, sfo1hullswere not closed yet, its lodiculesbegan to dehydrate and atrophy, but were still very full compared with those in the wild type. The F1and F2 of across with sterile lines 56S as female parent and the sfo1 as male parent wereused in genetic analysis and gene mapping, indicating that the sfo1 trait was controlled by a nuclear recessive gene. Using bulked segregation analysis (BSA) method, the SFO1 was located between the SSR marker RM1054 and Insert/Delete marker ZTQ51 on the chromosome 5, with a physical distance of 113kb and including 15 candidate genes in this region. LOC_Os05g50890 and LOC_Os05g50900 were preliminary selected as candidate genes for SFO1. These results laid a foundation for the SFO1 gene cloning and functional research.

Key words: Rice (oryza sativa), floret opening, Lodicule, Gene mapping

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