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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (03): 343-353.doi: 10.3724/SP.J.1006.2017.00343


Genetic Diversity of ALK Gene and Its Association with Grain Gelatinization Temperature in Currently Cultivated Rice Landraces from Hani’s Terraced Fields in Yunnan Province

LI Chuang1,2,**, LIU Cheng-Chen1,2,**, ZHANG Chang-Quan1, ZHU Ji-Hui1, XU Xiao-Ying2, ZHAO Fu-Wei 3, HUANG Shao-Wen4, JIN Yin-Gen2,*,LIU Qiao-Quan1,*   

  1. 1Jiangsu Key Laboratory of Crop Genetics and Physiology / Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China; 2 College of Biological Science and Technology, Yangzhou University, Yangzhou 225009, China; 3 Nanjing Institute of Environmental Sciences of Ministry of Environmental Protection, Nanjing 210042, China; 4 Yunnan Honghe University, Mengzi 661100, China
  • Received:2016-06-14 Revised:2016-09-18 Online:2017-03-12 Published:2016-09-29
  • Contact: 刘巧泉, E-mail: qqliu@yzu.edu.cn; 金银根, E-mail: ygenjin@hotmail.com
  • Supported by:

    This study was supported by the National Basic Research Program of China (2013CBA01402), the National Natural Science Foundation of China (31561143008, 31300324)and the Natural Sciences Research Project of Higher Learning Institution in Jiangsu Province (13KJB180028).


Yunnan province is one of the centers for genetic diversity of cultivated rice in Asia. A total of 111 rice landraces were collected from Hani’s rice terraces of Yunnan, and their population structure was analyzed by using 51 SSR molecular markers. The 111 rice landraces were divided into japonica group (group I) and indica group (group II); however, each of the two groups was not completely clustered according to its geographical origin. Most of the collected landraces, accounting for 83%, were indica. Gelatinization temperature (GT) is an important parameter affecting rice cooking and eating quality. The GT of rice flour was measured, which was widely different among the collected rice varieties, showing four classed very low (< 66°C), low (66-70°C), high (70-74°C) and very high ( >74°C). Meanwhile, ALK gene, also known as SSSIIa and controlling GT, was sequenced and compared, showing ten haploid types of the ALK gene among tested samples. Association analysis, revealed that GT is mainly controlled by the combination of three SNPs of ALK gene. Among them, the A-GC or G-TT combination type controls the very low and low GT, while the combination of G-GC controls high and very high GT. SNP3402 type T is associated with high and very high GT. It was also noticed that most of the varieties from indica group tended to contain the G-GC mutation and thus showed a relative high GT compared with those from the japonica group. The results have a meaningful value for rice molecular breeding and germplasm resources protection.

Key words: Oryza sativa L., ALK gene, Genetic diversity, Hani’s terrace, Gelatinization temperature

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