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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (08): 1183-1190.doi: 10.3724/SP.J.1006.2015.01183

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Mapping of Powdery Mildew Resistance Gene MlDH155 in Hexaploid Wheat DH155 and Its Transfer by Marker Assisted Selection

GUAN Chang-Ying**,GUO Jun**,XUE Feng-Bo,ZHANG Guang-Xu,WANG Hong-Wei,LI An-Fei,KONG Ling-Rang*   

  1. State Key Laboratory of Crop Biology / Shandong Agricultural University, Tai’an 271018, China?
  • Received:2015-01-28 Revised:2015-05-04 Online:2015-08-12 Published:2015-06-03

Abstract:

Hexaploid wheat (Triticum aestivum L.) line DH155 is highly resistant to wheat powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt). To identify the Bgt resistance gene(s) in DH155, we developed an F2 population and its derived F2:3 families by crossing the resistant line DH155 with the susceptible line SN2890. The segregation ratios indicated that the seedling resistance to Bgt E09 in DH155 was controlled by a single dominant gene, which was tentatively designated MlDH155. By bulked segregation analysis, two codominant SSR markers, Xcfd81 and Xcfd18, were identified to be linked to MlDH155. To identify the closely linked markers to the targeted gene, we developed five new molecular markers based on the published D genome sequences of Chinese Spring and Aegilops tauschii, which permitted mapping of MlDH155 within an interval of 1.0 cM, flanked by XsdauK525 and XsdauK527. The Pm resistant line DH155 was crossed with two elite wheat lines (HB133-4 and Han 10) but susceptible to powdery mildew. Subsequently, two powdery mildew resistant lines with the genetic background of HB133-4 and one resistant line with Han 10 background were developed by genotypic and phenotypic selection, which were designated by the name of SDAU2100, SDAU2101 and SDAU2102, respectively. Among the 14 Bgt isolates tested at the seedling stage, DH155 was resistant to 13 and susceptible to 1 isolates. The virulence pattern of these Bgt isolates on DH155 was similar to that of the known powdery mildew resistance gene Pm2, but the reactions of DH155 to two Bgt isolates differed from those of Ulka/8*Cc carrying Pm2. Compared to previous studies about Pm2, MlDH155 was most likely to be either the same as or an allele of Pm2.

Key words: Triticum aestivum, DH155, Powdery mildew, Resistance gene, Molecular marker

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