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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (08): 1183-1190.doi: 10.3724/SP.J.1006.2015.01183


Molecular Mapping of Powdery Mildew Resistance Gene MlDH155 in Hexaploid Wheat DH155 and Its Transfer by Marker Assisted Selection

GUAN Chang-Ying**,GUO Jun**,XUE Feng-Bo,ZHANG Guang-Xu,WANG Hong-Wei,LI An-Fei,KONG Ling-Rang*   

  1. State Key Laboratory of Crop Biology / Shandong Agricultural University, Tai’an 271018, China?
  • Received:2015-01-28 Revised:2015-05-04 Online:2015-08-12 Published:2015-06-03


Hexaploid wheat (Triticum aestivum L.) line DH155 is highly resistant to wheat powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt). To identify the Bgt resistance gene(s) in DH155, we developed an F2 population and its derived F2:3 families by crossing the resistant line DH155 with the susceptible line SN2890. The segregation ratios indicated that the seedling resistance to Bgt E09 in DH155 was controlled by a single dominant gene, which was tentatively designated MlDH155. By bulked segregation analysis, two codominant SSR markers, Xcfd81 and Xcfd18, were identified to be linked to MlDH155. To identify the closely linked markers to the targeted gene, we developed five new molecular markers based on the published D genome sequences of Chinese Spring and Aegilops tauschii, which permitted mapping of MlDH155 within an interval of 1.0 cM, flanked by XsdauK525 and XsdauK527. The Pm resistant line DH155 was crossed with two elite wheat lines (HB133-4 and Han 10) but susceptible to powdery mildew. Subsequently, two powdery mildew resistant lines with the genetic background of HB133-4 and one resistant line with Han 10 background were developed by genotypic and phenotypic selection, which were designated by the name of SDAU2100, SDAU2101 and SDAU2102, respectively. Among the 14 Bgt isolates tested at the seedling stage, DH155 was resistant to 13 and susceptible to 1 isolates. The virulence pattern of these Bgt isolates on DH155 was similar to that of the known powdery mildew resistance gene Pm2, but the reactions of DH155 to two Bgt isolates differed from those of Ulka/8*Cc carrying Pm2. Compared to previous studies about Pm2, MlDH155 was most likely to be either the same as or an allele of Pm2.

Key words: Triticum aestivum, DH155, Powdery mildew, Resistance gene, Molecular marker

[1]庄巧生. 中国小麦品种改良及系谱分析. 北京, 中国农业出版社, 2003

Zhuang Q S. Wheat Improvement and Pedigree Analysis in China. Beijing: China Agriculture Press, 2003 (in Chinese)

[2]何中虎, 兰彩霞, 陈新民, 邹裕春, 庄巧生, 夏先春. 小麦条锈病和白粉病成株抗性研究进展与展望. 中国农业科学, 2011, 44: 2193–2215

He Z H, Lan C X, Chen X M, Zou Y C, Zhuang Q S, Xia X C. Progress and perspective in research of adult-plant resistance to stripe rust and powdery mildew in wheat. Sci Agric Sin, 2011, 44: 2193–2215 (in Chinese with English abstract)

[3]Everts K L, Leath S, Finney P L. Impact of powdery mildew on milling and baking quality of soft red winter wheat. Plant Dis, 2001, 85: 423–429

[4]Conner R L, Kuzyk A D, Su H. Impact of powdery mildew on the yield of soft white spring wheat cultivars. Can J Plant Sci, 2003, 83: 725–728

[5]Huang X Q, Röder M S. Molecular mapping of powdery mildew resistance genes in wheat: a review. Euphytica, 2004, 137: 203–223

[6]Huang X Q, Hsam S L K, Zeller F J, Wenzel G, Mohler V. Molecular mapping of the wheat powdery mildew resistance gene Pm24 and marker validation for molecular breeding. Theor Appl Genet, 2000, 101: 407–414

[7]McIntosh R A, Dubcovsky J, Rogers W J, Morris C, Appels R, Xia X C. Catalogue of gene symbols for wheat: 2013–2014. (http://www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement2013.pdf)

[8]Xiao M G, Song F J, Jiao J F, Wang X M, Xu H X, Li H J. Identification of the gene Pm47 on chromosome 7BS conferring resistance to powdery mildew in the Chinese wheat landrace Hongyanglazi. Theor Appl Genet, 2013, 126: 1397–1403

[9]周阳, 何中虎, 张改生, 夏兰琴, 陈新民, 高永超, 井赵斌, 于广军. 1BL/1RS易位系在我国小麦育种中的应用. 作物学报, 2004, 30: 531–535

Zhou Y, He Z H, Zhang G S, Xia L Q, Chen X M, Gao Y C, Jing Z B, Yu G J. Utilization of 1BL/1RS translocation in wheat breeding in China. Acta Agron Sin, 2004, 30: 531–535 (in Chinese with English abstract)

[10]Hsam, S, Zeller F, Bélanger R, Bushnell W, Dik A, Carver T. Breeding for powdery mildew resistance in common wheat (Triticum aestivum L.). In: Bélanger R R, Bushnell W R, Dik A J, Carver T L W eds., The Powdery Mildews: a Comprehensive Treatise, St. Paul, MN, USA, 2002. pp 219–238

[11]韩利明, 张勇, 彭惠茹, 乔文臣, 何明琦, 王洪刚, 曲延英, 刘春来, 何中虎. 从产量和品质性状的变化分析北方冬麦区小麦品种抗热性. 作物学报, 2010, 36: 1538–1546

Han L M, Zhang Y, Peng H R, Qiao W C, He M Q, Wang H G, Qu Y Y, Liu C L, He Z H. Analysis of heat resistance for cultivars from north China winter wheat region by yield and quality traits. Acta Agron Sin, 2010, 36: 1538–1546 (in Chinese with English abstract)

[12]Briggle L W. Near-isogenic lines of wheat with genes for resistance to Erysiphe graminis f. sp. tritici. Crop Sci, 1969, 9: 70–72

[13]Gao H D, Zhu F F, Jiang Y J, Wu J Z, Yan W, Zhang Q F, Jacobi A, Cai SB. Genetic analysis and molecular mapping of a new powdery mildew resistant gene Pm46 in common wheat. Theor Appl Genet, 2012, 125: 967–973

[14]盛宝钦. 用反应型记载小麦苗期白粉病. 植物保护, 1988, (1): 49

Sheng B Q. Scoring powdery mildew with infection type at wheat seedling stage. Plant Prot, 1988 (1): 49 (in Chinese)

[15]Kong L R, Cambron S E, Ohm H W. Hessian fly resistance genes H16 and H17 are mapped to a resistance gene cluster in the distal region of chromosome 1AS in wheat. Mol Breed, 2008, 21: 183–194

[16]The International Wheat Genome Sequencing Consortium (IWGSC). A chromosome-based draft sequence of the hexaploid bread wheat (Triticum aestivum) genome. Science, 2014, doi: 10.1126/science.1251788

[17]Jia J, Zhao S, Kong X, Li Y, Zhao G, He W, Appels R, Pfeifer M, Tao Y, Zhang X, Jing R, Zhang C, Ma Y, Gao L, Gao C, Spannagl M, Mayer K, Li D, Pan S, Zheng F, Hu Q, Xia X, Li J, Liang Q, Chen J, Wicker T, Gou C, Kuang H, He G, Luo Y, Keller B, Xia Q, Lu P, Wang J, Zou H, Zhang R, Xu J, Gao J, Middleton C, Quan Z, Liu G, Wang J, International Wheat Genome Sequencing Consortium, Yang H, Liu X, He Z, Mao L, Wang J. Aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation. Nature, 2013, 496: 91–95

[18]Kosambi D D. The estimation of map distance from recombination values. Ann Eugen, 1944, 12: 172–175

[19]Lincoln S, Daly M, Lander E. Constructing genetic maps with Mapmaker/ EXP3.0. White head Institute Techn Rep, 1992

[20]Miranda L M, Murphy J P, Marshall D, Leath S. Pm34: a new powdery mildew resistance gene transferred from Aegilops tauschii Coss. to common wheat (Triticum aestivum L.). Theor Appl Genet, 2006, 113: 1497–1504

[21]Miranda L M, Murphy J P, Marshall D, Cowger C, Leath S. Chromosomal location of Pm35, a novel Aegilops tauschii derived powdery mildew resistance gene introgressed into common wheat (Triticum aestivum L.). Theor Appl Genet, 2007, 114: 1451–1456

[22]Li T, Zhang Z Y, Hu Y K, Duan X Y, Xin Z Y. Identification and molecular mapping of a resistance gene to powdery mildew from the synthetic wheat line M53. J Appl Genet, 2011, 52: 137–143

[23]Pugsley A T, Carter M V. The resistance of twelve varieties of Triticum vulgare to Erysiphe graminis tritici. Aust J Biol Sci, 1953, 6: 335–346

[24]McIntosh R A, Baker E P. Cytogenetical studies in wheat: IV. Chromosome location and linkage studies involving the Pm2 locus for powdery mildew resistance. Euphytica, 1970, 19: 71–77

[25]Qiu Y C, Sun X L, Zhou R H, Kong X Y, Zhang S S, Jia J Z. Identification of microsatellite markers linked to powdery mildew resistance gene Pm2 in wheat. Cereal Res Commun, 2006, 34: 1267–1273

[26]Huang J, Zhao Z H, Song F J, Wang X M, Xu H X, Huang Y, An D G, Li H J. Molecular detection of a gene effective against powdery mildew in the wheat cultivar Liangxing 66. Mol Breed, 2012, 30: 1737–1745

[27]Ma P, Xu H, Luo Q, Qie Y, Zhou Y, Xu Y, Han H, Li L, An D. Inheritance and genetic mapping of a gene for seedling resistance to powdery mildew in wheat line X3986-2. Euphytica, 2014, 200: 149–157

[28]Hsam S, Huang X, Ernst F, Hartl L, Zeller F. Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.): 5. Alleles at the Pm1 locus. Theor Appl Genet, 1998, 96: 1129–1134

[29]Singrun C H, Hsam S, Hartl L, Zeller F, Mohler V. Powdery mildew resistance gene Pm22 in cultivar Virest is a member of the complex Pm1 locus in common wheat (Triticum aestivum L. em Thell.). Theor Appl Genet, 2003, 106: 1420–1424

[30]Schmolke M, Mohler V, Hartl L, Zeller F J, Hsam S. A new powdery mildew resistance allele at the Pm4 wheat locus transferred from einkorn (Triticum monococcum). Mol Breed, 2012, 29: 449–456

[31]张海泉. 小麦抗白粉病分子育种研究进展. 中国生态农业学报, 2008, 16: 1060–1066

Zhang H Q. Research advances in molecular breeding of powdery mildew resistance of wheat. Chin J Eco-Agric, 2008, 16: 1060–1066 (in Chinese with English abstract)

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