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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (08): 1191-1200.doi: 10.3724/SP.J.1006.2015.01191


Stress-activated Protein Kinase OsSAPK2 Involved in Regulating Resistant Response to Xanthomonas oryzae pv. oryzae in Rice

HU Dan-Dan1,2,ZHANG Fan2,HUANG Li-Yu2,ZHUO Da-Long1,2,ZHANG Fan2,ZHOU Yong-Li2,*,SHI Ying-Yao2,*,LI Zhi-Kang2   

  1. 1 College of Agronomy, Anhui Agricultural University, Hefei 230036, China; 2 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2015-01-08 Revised:2015-05-04 Online:2015-08-12 Published:2015-06-03
  • Contact: 石英尧,E-mail: shiyy123@163.com, Tel: 0551-65786213; 周永力,E-mail: zhouylcaas@126.com E-mail:hudandan_ahau@163.com


Sucrose nonfermenting1-related protein kinase2 (SnRK2), also known as stress-activated protein kinase (OsSAPKs), plays an important role in signal transduction. In this study, we analyzed the structure and function of OsSAPK2 in response to Xanthomonas oryzae pv. oryzae (Xoo) infection. The result suggested that OsSAPK2 is a member of Kulik’s II group like OsSAPK1, OsSAPK3 and located in nucleus and cytoplasm. OsSAPK2 and disease-resistant genes OsLRR1, OsHIR1 were down regulated in OsSAPK2-RNAi transgenic rice, while disease-related gene OsMAPK5 was up regulated. Compared with non-transgenic plants, transgenic plants were more susceptible to Xoo infection. OsSAPK2 could activate itself and interact with several stress-related proteins. These results indicate that OsSAPK2 might be involved in the regulation of resistance response by regulating the expression of OsLRR1, OsHIR1, OsMAPK5 and interacting with stress-related proteins.

Key words: OsSAPK2, Oryza sativa, Bacterial blight, RNAi

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