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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (11): 1682-1691.doi: 10.3724/SP.J.1006.2015.01682

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Overexpression of Vacuole H+-ATPase E Subunit Gene SiVHA-E from Foxtail Millet Enhances Salt Resistance in Transgenic Arabidopsis thaliana

FENG Lu1,2,ZHONG Li2,3,CHEN Dan-Dan4,MA You-Zhi2,XU Zhao-Shi2,LI Lian-Cheng2,ZHOU Yong-Bin2,CHEN Ming2,*,ZHANG Xiao-Hong1,*   

  1. 1 College of Life Science, Northwest A&F University, Yangling 712100, China; 2 Institute of Crop Science, Chinese Academy of Agricultural Science / National Key Facility for Crop Gene Response and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crop, Ministry of Agriculture, Beijing 100081, China; 3 Guizhou Provincial Institute of Grass Industry, Guiyang 550006, China; 4 College of Agronomy, Northwest A&F University, Yangling 712100, China
  • Received:2015-02-11 Revised:2015-06-01 Online:2015-11-12 Published:2015-06-29

Abstract:

The V-H+-ATPase plays an important role in processes of plant growth, development and response to stresses. In this research, SiVHA-E, a V-H+-ATPase E subunit gene, was cloned from millet by the Blast analysis against GenBank database. Phylogenetic tree showed that the gene belongs to E1/E3 subgroup and is close with ZmVHA-EL, a V-H+-ATPase E subunit from maize. The quantitative Real-time PCR (qRT-PCR) analysis revealed that the expression levels of SiVHA-E were up-regulated under treatments of high-salt, exogenous MeJA, SA, and ABA hormones, while down-regulated under stresses of cold and low nitrogen. Protein subcellular localization analysis using protoplast showed that SiVHA-E is located on tonoplast. The results of salt tolerance assay showed that the germination rate of SiVHA-E transgenic lines was significantly higher than that of wild type plant under salt stress. During seedlings period, the root lengths were significantly longer as well as fresh weight and survival rate were significantly higher in transgenic lines than in wild type plant under salt treatment. Compared with wild type plant, transgenic plant reduced the content of Na+ and increased the relative water content inside cells. In addition, the results of germination experiment used ABA showed that SiVHA-E transgenic Arabidopsis was more sensitive to ABA than wild type plant during post-germination. In short, overexpressing SiVHA-E in transgenic Arabidopsis lines enhances salt tolerance, which might be relates to positive regulation of ABA signaling pathway or reduction of Na+ accumulation and water loss in transgenic plants.

Key words: Foxtail millet, V-type H+-ATPase E subunit gene, Salt stress tolerance, Mechanism

[1]Agarwal S, Pandey V. Antioxidant enzyme responses to NaCl stress in Cassia angustifolia. Biol Plant, 2004, 48: 555–560



[2]Niu X M, Narasimhan M L, Salzman R A, Bressan R A, Hasegawa P M. NaCl regulation of plasma membrane H+-ATPase gene expression inaglycophyte and halophyte. Plant Physiol, 1993, 103: 713–718



[3]Zhu J K. Plant salt tolerance. Trends Plant Sci, 2001, 6: 66–71



[4]Li P H, Chen M, Wang B S. Effect of K+ nutrition on growth and activity of leaf tonoplast V-H+-ATPase and V-PPase of suaed salsa under NaCl stress. Acta Bot Sin, 2002, 44: 433–440



[5]Sze H, Schumacher K, Müller LM, Padmanaban S, Taiz L. A simple nomenclature for a complex proton pump: VHA genes encode the vacuolar H+-ATPase. Trends Plant Sci, 2002, 7: 157–161



[6]Kluge C, Lahr L, Hanitzsch L, Bolte S, Golldack D, Dietz K J. New insight into the structure and regulation of the plant vacuolar V-ATPase. J Bioenerg Biomemb, 2003, 35: 377–388



[7]Omri D, Felix F, Nelson N. Crystal structure of yeast V-ATPase subunit C reveals its stator function. EMBO Rep, 2004, 5: 1148–1152



[8]Dettmer J, Liu T Y, Schumacher K. Functional analysis of Arabidopsis V-ATPase subunit VHA-E isoforms. Eur J Cell Biol, 2010, 89: 152–156



[9]Dietz K J, Tavakoli N, Kluge C, Mimura T, Sharma S S, Harris G C, Chardonnens A N, Golldack D. Significance of the V-type ATPase for the adaptation to stressful growth conditions and its regulation on the molecular and biochemical level. J Exp Bot, 2001, 52: 1969–1980



[10]Wang B S, Lttge U, Ratajczak R. Effects of salt treatment and osmotic stress on V-ATPase and V-PPase in leaves of the halophyte Suaeda salsa. J Exp Bot, 2001, 52: 2355–2365



[11]夏朝晖, 陈珈. 胁迫反应中的液泡膜H+-ATPase. 植物生理学通讯, 1998, 34: 168–174



Xia Z H, Chen J. Type H+-ATPase in responses to stresses. 1998, 34: 168–174 (in Chinese)



[12]He X, Huang X, Shen Y, Huang Z. Wheat V-H+-ATPase subunit genes significantly affect salt tolerance in Arabidopsis thaliana. PLoS One, 2014, 9: e86982



[13]Zhang X H, Li B, Hu Y G, Chen L, Min D H. The wheat E subunit of V-type H+-ATPase is involved in the plant response to osmotic stress. Intl J Mol Sci, 2014, 15: 16196–16210



[14]Barton L, Newsome S D, Chen F H, Wang H, Guilderson T P, Bettinger R L. Agricultural origins and the isotopic identity of domestication in northern China. Proc Natl Acad Sci USA, 2009, 106: 5523–5528



[15]Bettinger R L, Barton L, Morgan C. The origins of food production in north China: a different kind of agricultural revolution. Evol Anthropol, 2010, 19: 9–21



[16]Doust A N, Kellogg E A, Devos K M, Bennetzen J L. Foxtail millet: a sequence-driven grass model system. Plant Physiol, 2009, 149: 137–141



[17]Brutnell T P, Wang L, Swartwood K, Goldschmidt A, Jackson D, Zhu X G, Kellogg E, Van Eck J. Setariaviridis: a model for C4 photosynthesis. Plant Cell, 2010, 22: 2537–2544



[18]Li P, Brutnell T P. Setariaviridis and Setariaitalica, model genetic systems for the Panicoid grasses. J Exp Bot, 2011, 62: 3031–3037



[19]Lata C, Gupta S, Prasad M. Foxtail millet: a model crop for genetic and genomic studies in bioenergy grasses. Crit Rev Biotechnol, 2013, 33: 328–343



[20]Yoo S D, Cho Y H, Sheen J. Arabidopsis mesophyll protoplasts:a versatile cell system for transient gene expression analysis. Nat Prot, 2007, 2: 1565–1572



[21]Xu Z S, Ni Z Y, Liu L, Nie L N, Li L C, Chen M, Ma Y Z. Characterization of the TaAI DFagene encoding a CRT/DRE-binding factor responsive to drought, high-salt, and cold stress in wheat. Mol Genet Genom, 2008, 280: 497–508



[22]Ding L, Zhu J K. Reduced Na+ uptake in the NaCl-hypersensitive sos1 mutant of Arabidopsis thaliana. Plant Physiol, 1997, 113: 795–799



[23]李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000. pp 119–263



Li H S. Principle and Technology of Plant Physiology and Biochemistry Experiment. Beijing: Higher Education Press, 2000. pp 119–263 (in Chinese)



[24]Zhao Q, Zhao Y J, Zhao B C, Ge R C, Li M, Shen Y Z, Huang Z J. Cloning and functional analysis of wheat V-H+-ATPase subunit genes. Plant Mol Biol, 2009, 69: 33–46



[25]Ratajczak R. Structure, function and regulation of the plant vacuolar H+-translocating ATPase. Biochim Biophys Acta, 2000, 1465: 17–36



[26]Chinnusamy V, Zhu J H, Zhu J K. Salt stress signaling and mechanismas of plant salt tolerance. Genet Engin, 2006, 27: 141–177



[27]Greenway H, Munns R. Mechanisms of salt tolerance in nonhalophytes. Annu Rev Plant Physiol, 1980, 31: 149–190



[28]Barkla B J, Zingarelli L, Blumwald E, Smith J A C. Tonoplast Na+/H+ antiport activity and itsenergization by the vacuolar H+-ATPase in the hallophytic plant Mesembryanthemum crystallinum L. Plant Physiol, 1995, 109: 549–556



[29]Janicka-Russak M, K?obus G. Modification of plasmamembrane and vacuolar H+-ATPasesin response to NaCl and ABA. J Plant Physiol, 2007, 164: 295–302



[30]Zhi R, To P. Function of transport H+-ATPases in plant cell plasma and vacuolar membranes of maize under salt stress conditions and effect of adaptogenic preparations. Ukrainski? Biokhimicheski? Zhurnal, 2011, 83: 63–68



[31]Kasai M, Yamamoto Y, Maeshima M, Matsumoto H. In vivo treatment barley roots with vanadate increases vacuolar H+-translocating ATPase activity of the tonoplast-enriched membrane vesicles and the level of endogenous ABA. Plant Cell Physiol, 1994, 35: 291–295



[32]Bray E A. Plant responses to water deficit. Ttends Plant Sci, 1997, 2: 48–54



[33]Schroeder J I, Allen G J, Hugouvieux V, Kwak J M, Waner D. Guard cell signal tranduction. Annu Rev Plant Physiol Plant Mol Biol, 2001, 52: 627–65.



[34]Gaxiola R A, Li J S, Undurraga S, Dang L M, Allen G J, Alper S L, Fink G R. Drought- and salt-tolerant plants result from overexpression of the AVP1 H+-pump. Proc Natl Acad Sci USA, 2001, 98: 11444–11449



[35]Krebs M, Beyhl D, Gorlich E, Al-Rasheid A S, Marten I, Stierhof Y D, Hedrich R, Schumacher K. Arabidopsis, V-ATPase activity at the tonoplast is required for efficient nutrient storage but not for sodium accumulation. Proc Natl Acad Sci USA, 2010, 107: 3251–3256

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