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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (12): 1828-1835.doi: 10.3724/SP.J.1006.2015.01828


Genetic Diversity and Association Analysis of Protein Content in Weak Gluten Wheat Ningmai 9 and Its Derived Lines

JIANG Peng,ZHANG Ping-Ping,ZHANG Xu,CHEN Xiao-Ling,YAO Jin-Bao,MA Hong-Xiang*   

  1. Jiangsu Provincial Key Laboratory for Agrobiology / Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2015-03-27 Revised:2015-07-20 Online:2015-12-12 Published:2015-08-05


Ningmai 9 is an elite weak gluten wheat cultivar and an important breeding parent in the south area of Huai River Valley, and 15 new cultivars have been developed and released from Ningmai 9. This study aimed at dissecting the genetic mechanism and inheritance of protein content in Ningmai 9 and its derivatives. The kernel protein content (KPC) and flour protein content (FPC) of Ningmai 9 and its 117 derivatives were evaluated in the 20092010 and 20102011 growing seasons and the QTLs associated with KPC and FPC were identified by whole genomes screening with 185 SSR markers. The genetic similarity index ranged from 0.55 to 0.88 among Ningmai 9 and its derivatives. In the Neighbor-Joining cluster tree, Ningmai 9 was clustered with most first-generation lines first, then with the second-generation lines, and Yangfumai 4 of first-generation derivative was the last one to incorporate in. The first- and second-generation derivatives inherited 75.60% and 67.81% of Ningmai 9 alleles, respectively. Great variations of KPC and FPC were observed in the Ningmai 9 derived lines with the variation coefficients ranging from 5.07% to 7.28%. Six and five QTLs were identified to be associated with KPC and FPC, respectively, of which three and two were stably detected in both years. One QTL was associated with both KPC and FPC. Three stable QTLs, Xgwm539, Xwmc397, and Xwmc468, had negative effects on KPC or FPC in Ningmai 9 and might be used for quality improvement in weak-gluten wheat breeding.

Key words: Wheat, Protein content, Association analysis, Molecular markers

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