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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (03): 368-375.doi: 10.3724/SP.J.1006.2016.00368


Cloning and Deletion Analysis of GhRACK1 Promoter from Gossypium hirsutum

YANG Jiang-Tao,PANG Wei-Min,WANG Xu-Jing,LÜ Shao-Pu,TANG Qiao-Ling,WANG Zhi-Xing   

  1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2015-05-29 Revised:2015-11-20 Online:2016-03-12 Published:2015-12-18
  • Contact: 王旭静, E-mail: wangxujing@caas.cn, Tel: 010-82106124; 王志兴, E-mail: wangcotton@126.com, Tel: 010-82106102 E-mail:JT_Y1990@163.com
  • Supported by:

    This study was supported by the National Major Project of Breeding for New Transgenic Organisms (2014zx08010-005).


The absence of fiber specific promoter with future prospect is one of the main factors to restrict the development of genetic engineering in cotton fiber improvement. A 1987 bp length promoter sequence of Gossypium hirsutum GhRACK1 gene, which encodes receptor for activated C kinase 1 and precedantly expresses in fiber, was cloned by combination of inverse PCR and touchdown PCR method. Sequence analysis showed there were lots of promoter regulation elements such as Cis acting factor and the tissue specific regulation elements. The full-length GhRACK1-P and truncations from –600 to –1 bp, –1036 to –1 bp, –1260 to –1 bp and –1620 to –1 bp were obtained by PCR method. Each of the truncations was fused with gus gene and inserted into plant expression vectors pCamBIA2300. All constructs were transformed into Nicotiana tabacum var. NC89 through Agrobacterium-mediated transformation method. GUS histochemical assay showed that the full-length GhRACK1-P promoter was expressed in root and exhibited a tissue-specific expression manner. All of the truncations were expressed in root, leaf and pollen and exhibited a constitutive expression manner. Because there is the similar developmental mechanism between cotton fiber and tobacco young root or trichome, the results indicate that GhRACK1- P may be a fiber specific expression promoter.

Key words: Preferential expression in fiber, GhRACK1 promoter, Clone, Deletion analysis

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