Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (03): 368-375.doi: 10.3724/SP.J.1006.2016.00368

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Deletion Analysis of GhRACK1 Promoter from Gossypium hirsutum

YANG Jiang-Tao,PANG Wei-Min,WANG Xu-Jing,LÜ Shao-Pu,TANG Qiao-Ling,WANG Zhi-Xing   

  1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2015-05-29 Revised:2015-11-20 Online:2016-03-12 Published:2015-12-18
  • Contact: 王旭静, E-mail: wangxujing@caas.cn, Tel: 010-82106124; 王志兴, E-mail: wangcotton@126.com, Tel: 010-82106102 E-mail:JT_Y1990@163.com
  • Supported by:

    This study was supported by the National Major Project of Breeding for New Transgenic Organisms (2014zx08010-005).

Abstract:

The absence of fiber specific promoter with future prospect is one of the main factors to restrict the development of genetic engineering in cotton fiber improvement. A 1987 bp length promoter sequence of Gossypium hirsutum GhRACK1 gene, which encodes receptor for activated C kinase 1 and precedantly expresses in fiber, was cloned by combination of inverse PCR and touchdown PCR method. Sequence analysis showed there were lots of promoter regulation elements such as Cis acting factor and the tissue specific regulation elements. The full-length GhRACK1-P and truncations from –600 to –1 bp, –1036 to –1 bp, –1260 to –1 bp and –1620 to –1 bp were obtained by PCR method. Each of the truncations was fused with gus gene and inserted into plant expression vectors pCamBIA2300. All constructs were transformed into Nicotiana tabacum var. NC89 through Agrobacterium-mediated transformation method. GUS histochemical assay showed that the full-length GhRACK1-P promoter was expressed in root and exhibited a tissue-specific expression manner. All of the truncations were expressed in root, leaf and pollen and exhibited a constitutive expression manner. Because there is the similar developmental mechanism between cotton fiber and tobacco young root or trichome, the results indicate that GhRACK1- P may be a fiber specific expression promoter.

Key words: Preferential expression in fiber, GhRACK1 promoter, Clone, Deletion analysis

[1]Huang G Q, Gong S Y, Xu W L, Li W, Li P, Zhang C J, Li D D, Zheng Y, Li F G, Li X B. A fasciclin-like arabinogalactan protein, GhFLA1, is involved in fiber initiation and elongation of cotton. Plant Physiol, 2013, 161: 1278–1290

[2]Lee J, Burns T H, Light G, Sun Y, Fokar M, Kasukabe Y, Fujisawa K, Maekawa Y, Allen R D. Xyloglucan endotransglycosylase/hydrolase genes in cotton and their role in fiber elongation. Planta, 2010, 232: 1191–1205

[3]孟亚雄, 张金文, 马小乐, 张金林, 仲军, 王化俊. 棉纤维特异启动子LTP12驱动的基因phaB、phaC双价载体构建. 草业科学, 2010, 19(3): 170–176

Meng Y X, Zhang J W, Ma X L, Zhang J L, Zhong J, Wang H J. Constructing expression vector of genes phaB and phaC derived by specific promoter LTP12 for cotton fiber. Acta Pratac Sin, 2010, 19(3): 170–176 (in Chinese with English abstract)

[4]吕少溥, 王旭静, 唐巧玲, 王志兴. 棉纤维特异基因及其启动子的研究进展. 生物技术进展, 2014, 4(1): 1–6

Lü S P, Wang X J, Tang Q L, Wang Z X. Research progress of fiber-specific gene and promoter from cotton. Curr Biotech, 2014, 4(1): 1–6 (in Chinese with English abstract)

[5]Shan C M, Shangguan X X, Zhao B, Zhang X F, Chao L M, Yang C Q, Wang L J, Zhu H Y, Zeng Y D, Guo W Z, Zhou B L, Hu G J, Guan X Y, Chen Z J, Wendel J F, Zhang T Z, Chen X Y. Control of cotton fibre elongation by a homeodomain transcription factor GhHOX3. Nat Commun, 2014, 5: 5519

[6]Ron D, Mochly-Rosen D. Agonists and antagonists of protein kinase C function, derived from its binding proteins. J Biol Chem, 1994, 269: 21395– 21398

[7]Ishida S, Takahashi Y, Naqata T. Isolation of cDNA of an auxin-regulated gene encoding a G protein beta subunit-like protein from tobacco BY-2 cells. Proc Natl Acad Sci USA, 1993, 90: 11152–11156

[8]Iwasaki Y, Komano M, Ishikawa A, Sasaki T, Asahi T. Molecular cloning and characterization of cDNA for a rice protein that contains seven repetitive segments of the Trp-Asp forty-amino-acid repeat (WD-40 repeat). Plant Cell Physiol, 1995, 36:505–510

[9]Vahlkamp L, Palme K. AtArcA, accession No. U77381, the Arabidopsis thaliana homolog of the tobacco ArcA gene (PGR97-145). Plant Physiol, 1997, 115:863

[10]McKhann H I, Frugier F, Petrovics G, de la Peña T, Jurkevitch E, Brown S, Kondorosi E, Kondorosi A, Crespi M. Cloning of a WD-repeat-containing gene from alfalfa (Medicago sativa): a role in hormone-mediated cell division. Plant Mol Biol, 1997, 34:771–780

[11]Kwak J M, Kim S A, Lee S K, Oh S A, Byoun C H, Han J K, Nam H G. Insulin-induced maturation of Xenopus oocytes is inhibited by microinjection of a Brassica napus cDNA clone with high similarity to a mammalian receptor for activated protein kinase C. Planta, 1997, 201:245–251

[12]庞伟民, 靳茜, 王旭静, 杨江涛, 吕少溥, 唐巧玲, 王志兴. 陆地棉纤维优势表达基因GhRACK1的克隆与序列分析. 生物技术进展, 2015, 5: 366–370

Pang W M, Jin X, Wang X J, Yang J T, Lü S P, Tang Q L, Wang Z X. Cloning and sequence analysis of GhRACK1 gene dominant expressed in fiber from Gossypium hirsutum. Curr Biotech, 2015, 5: 366–370 (in Chinese with English abstract)

[13]Jefferson R A, Kavanagh T A, Bevan M W. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J, 1987, 6: 3901–3907

[14]Taniguchi M, Izawa K, Ku M S, Lin J H, Saito H, Ishida Y, Ohta S, Komari T, Matsuoka M, Sugiyama T. The promoter for the maize C4 pyruvate, orthophosphate dikinase gene directs cell- and tissue-specific transcription in transgenic maize plants. Plant Cell Physiol, 2000, 41: 42–48

[15]Won S K, Lee Y J, Lee H Y, Heo Y K, Cho M, Cho H T. Cis-element- and transcriptome-based screening of root hair-specific genes and their functional characterization in Arabidopsis. Plant Physiol, 2009, 150: 1459–1473

[16]Nitz I, Berkefeld H, Puzio P S, Grundler F M. Pyk10, a seedling and root specific gene and promoter from Arabidopsis thaliana. Plant Sci, 2001, 161: 337–346

[17]王旭静, 李为民, 唐巧玲, 贾士荣, 王志兴. 中棉(Gossypium arboreum)光锈导基因Gacab启动子在转基因烟草中的功能缺失分析. 作物学报, 2009, 35: 1006–1012

Wang X J, Li W M, Tang Q L, Jia S R, Wang Z X. Function deletion analysis of light-induced Gacab promoter from Gossypium arboreum in transgenic tobacco. Acta Agron Sin, 2009, 35: 1006–1012 (in Chinese with English abstract)

[18] Suo J F, Liang X E, Li P, Zhang Y S, Xue Y B. Identification of GhMYB109 encoding a R2R3 MYB transcription factor that expressed specifically in fiber initials and elongating fibers of cotton (Gossypium hirsutum L.). Biochem Biophys Acta, 2003, 1630: 25–34

[19] Wang S, Wang J W, Yu N, Li C H, Luo B, Gou J Y, Wang L J, Chen X Y. Control of plant trichome development by a cotton fiber MYB gene. Plant Cell, 2004, 16: 2323–2334

[20]上官小霞, 吴霞, 李燕娥. 棉花GhRDL1基因启动子分析. 华北农学报, 2010, 25(1): 6–10

Shangguan X X, Wu X, Li Y E. Analysis of the promoter of cotton GhRDL1 gene. Acta Agric Boreali-Sin, 2010, 25(1): 6–10 (in Chinese with English abstract)

[21] Shangguan X X, Xu B, Yu Z X. Promoter of a cotton fiber MYB gene functional in trichomes of Arabidopsis and Glandular trichomes of tobacco. J Exp Bot, 2008, 59: 3533–3542
[1] LI Hai-Fen, WEI Hao, WEN Shi-Jie, LU Qing, LIU Hao, LI Shao-Xiong, HONG Yan-Bin, CHEN Xiao-Ping, LIANG Xuan-Qiang. Cloning and expression analysis of voltage dependent anion channel (AhVDAC) gene in the geotropism response of the peanut gynophores [J]. Acta Agronomica Sinica, 2022, 48(6): 1558-1565.
[2] YANG Xin, LIN Wen-Zhong, CHEN Si-Yuan, DU Zhen-Guo, LIN Jie, QI Jian-Min, FANG Ping-Ping, TAO Ai-Fen, ZHANG Li-Wu. Molecular identification of a geminivirus CoYVV and screening of resistant germplasms in jute [J]. Acta Agronomica Sinica, 2022, 48(3): 624-634.
[3] Tao FENG,Chun-Yun GUAN. Cloning and characterization of phytochrome interacting factor 4 (BnaPIF4) gene from Brassica napus L. [J]. Acta Agronomica Sinica, 2019, 45(2): 204-213.
[4] Tao FENG,Chun-Yun GUAN. Cloning and Characterization of Brassinazole-resistant (BnaBZR1 and BnaBES1) CDS from Brassica napus L. [J]. Acta Agronomica Sinica, 2018, 44(12): 1793-1801.
[5] HAO Xin-Yuan,YUEChuan,TANG Hu,QIAN Wen-Jun,WANG Yu-Chun,WANG Lu, WANG Xin-Chao,YANG Ya-Jun. Cloning of β-amylase Gene (CsBAM3) and ItsExpression ModelResponseto Cold Stress in Tea Plant [J]. Acta Agron Sin, 2017, 43(10): 1417-1425.
[6] YUAN Lian-Yu,CHEN Ying-Juan,WEI Xu,TONG Hua-Rong*. Cloning and Function Analysis of Metal Tolerance Gene (CsMTP11) in Tea Plant (Camellia sinensis L. O. Kuntze) [J]. Acta Agron Sin, 2017, 43(05): 708-717.
[7] ZHANG Li-Jia,ZHANG Yan,RONG Wei,YANG Jun,ZHANG Gui-Yin,WU Li-Qiang,LI Zhi-Kun,WU Jin-Hua,MA Zhi-Ying,WANG Xing-Fen. Cloning and Expression Analysis of a Functional Gene GbVWR Induced by Verticillium dahliae in Gossypium barbadense [J]. Acta Agron Sin, 2016, 42(12): 1779-1786.
[8] TAN Tai-Long,FENG Tao,LUO Hai-Yan,PENG Ye,LIU Rui-Yang,GUAN Chun-Yun. Cloning and Characterization of Phospholipids:Diacylglycerol Acyltransferase (BnPDAT1) cDNA from Brassica napus L. [J]. Acta Agron Sin, 2016, 42(05): 658-666.
[9] MA Li-Gong,ZHANG Yun-Hua,MENG Qing-Lin,SHI Feng-Mei,LIU Jia,LI Yi-Chu,WANG Zhi-Ying. Cloning and Function Analysis of Pathogenesis Related Protein Gene HaPR1 from Sunflower (Helianthus annuus) [J]. Acta Agron Sin, 2015, 41(12): 1819-1827.
[10] HE Hai-Wang,PAN Hua-Qing,ZHANG Nao-Dan,HE Long-Fei*. Chromolaena odorata Flavonoid 3’-hydroxylase Gene Cloning and Its Expression in Tobacco [J]. Acta Agron Sin, 2015, 41(03): 479-486.
[11] SONG Xiu-Peng,ZHANG Bao-Qing,HUANG Xing,YANG Li-Tao,LI Yang-Rui. Cloning and Expression of Sugarcane S-Adenosylmethionine Synthetase Gene ScSAM [J]. Acta Agron Sin, 2014, 40(06): 1002-1010.
[12] HEN Na,PAN Li-Juan,CHI Xiao-Yuan,CHEN Ming-Na,WANG Tong,WANG Mian,YANG Zhen,HU Dong-Qing,WANG Dao-Yuan,YU Shan-Lin. Cloning and Expression Analysis of Fructose-1,6-Bisphosphate Aldolase Gene AhFBA1 in Peanut (Arachis hypogaea L.) [J]. Acta Agron Sin, 2014, 40(05): 934-941.
[13] ZHANG Lei, LIU Zhi-Feng, WANG Pan-Rong. Isolation and Analysis of AGAMOUS Homologous Gene and Its Promoter from Vicia sativa L. [J]. Acta Agron Sin, 2011, 37(10): 1735-1742.
[14] WU Juan-Juan, WU Qian, YU De-Yue. Cloning and Characterization of GmAOS Gene and Its Promoter in Soybean (Glycine max) [J]. Acta Agron Sin, 2011, 37(02): 216-223.
[15] WANG Ming-Xia,GAO Xiang,CHEN Qi-Jiao,DONG Jian,ZHAO Wan-Chun,LI Yan-Liang et al. Gene Cloning and Sequence Analysis of γ-Gliadin Genes from Wheat Cultivar Shann 253 [J]. Acta Agron Sin, 2010, 36(3): 526-532.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!