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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (12): 1819-1827.doi: 10.3724/SP.J.1006.2015.01819


Cloning and Function Analysis of Pathogenesis Related Protein Gene HaPR1 from Sunflower (Helianthus annuus)

MA Li-Gong1,2,ZHANG Yun-Hua2,*,MENG Qing-Lin2,SHI Feng-Mei2,LIU Jia2,LI Yi-Chu2,WANG Zhi-Ying1,*   

  1. 1 College of Forestry, Northeast Forestry University, Harbin 150040, China; 2 Institute of Plant Protection, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
  • Received:2015-04-20 Revised:2015-07-20 Online:2015-12-12 Published:2015-08-11
  • Contact: 王志英, E-mail: zyw0451@sohu.com, Tel: 13069873855; 张匀华, E-mail: yhzhang9603@126.com, Tel: 13603639603 E-mail:maligong0@163.com
  • Supported by:

    This research was supported by the Modern Agro-industry Technology Research System (CARS-16) and the Agricultural Science and Technology Innovation Program of Heilongjiang Province (QN015).


Pathogenesis-related proteins are commonly used as markers of plant defense responses. The full-length cDNA of pathogenesis-related protein 1 (PR1) named HaPR1 in Helianthus annuus was cloned based on the transcriptome of H.annuus induced by Sclerotinia sclerotiorum, and its expression model and function were analyzed in this study. Sequence analysis showed that the cDNA of HaPR1 (GenBank No. KR071874) contained a 489 bp ORF encoding a protein of 162 amino acids residues with the molecular mass of 17.52 kD and theoretical pI of 8.19, HaPR1 possessed six conserved cysteine and four conserved allergen V5/Tpx-1 related domain. The HaPR1 was highly homologous with PR1 in other species. Real-time PCR analysis showed that expression level of HaPR1 was the highest in leaf, and was significantly induced by drought, salt stress, oxalic acid, S. sclerotiorum and its metabolites. Then the HaPR1 gene was transformed into tobacco by Agrobacterium tumefaciens to further verify its function. The results showed that the expression of HaPR1 improved the resistance of transgenic lines, and significantly increased SOD, POD, and CAT activities and reduced the content of MDA. It suggested that HaPR1 has a function of resistance to S. sclerotiorum.

Key words: Helianthus annuus, Pathogenesis-related protein 1, Gene clone, Function analysis

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