Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (05): 627-632.doi: 10.3724/SP.J.1006.2016.00627


Effect of ω-Secalin Gene Silencing on Processing Quality of Wheat 1B/1R Translocation Line

CHAI Jian-Fang*,WANG Hai-Bo*,MA Xiu-Ying,ZHANG Cui-Mian,DONG Fu-Shuang   

  1. Genetics and Physiology Institute, Hebei Academy of Agriculture and Forestry Sciences / Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, China
  • Received:2015-09-28 Revised:2016-03-02 Online:2016-05-12 Published:2016-03-11
  • Contact: 柴建芳, E-mail: chai87652130@163.com; 王海波, E-mail: nkywanghb@163.com
  • Supported by:

    This study was supported by the Natural Science Foundation of Hebei Province, China (C2014301005).


Omega-secalin is an important factor for the poor processing quality of wheat 1B/1R translocation lines. In this study, an expression vector of silencing ω-secalin gene was constructed and transferred into wheat variety Jinhe 9123 via Agrobacterium tumefaciens strain EH105. The three T0 transgenic plants obtained were consecutively multiplied after PCR validation until the T4 lines. Acid-PAGE assay indicated that total amount of ω-secalins in the transgenic lines decreased 53% on average compared with that in their respective control. In the three homozygous transgenic lines, gluten index, precipitation value, and stabilization time increased significantly, whereas the agronomic traits, such as plant height, grain number per spike, 1000-grain weight and plot yield, had no significant changes compared with those in the wild type. These results suggest that silencing ω-secalin genes can improve the processing quality of wheat 1B/1R translocation lines on the premise of not affecting grain yield.

Key words: Wheat 1B, 1R translocation line, Secalin, RNAi, Gene silencing, Processing quality

[1] 周阳, 何中虎, 张改生, 夏兰琴, 陈新民, 高永超, 井赵斌, 于广军. 1BL/1RS易位系在我国小麦育种中的应用. 作物学报, 2004,30: 531–535
Zhou Y, He Z H, Zhang G S, Xia L Q, Chen X M, Gao Y C, Jing Z B, Yu G J. Utilization of 1BL/1RS translocation in wheat breeding in China. Acta Agron Sin, 2004, 30: 531–535 (in Chinese with English abstract)

[2] 杨足君, 任正隆. 抗白粉病基因Pm8在四川小麦中遗传表达初步研究. 四川农业大学学报, 1997, 15: 452–456
Yang Z J, Ren Z L. Expression of gene Pm8 for resistance to powdery mildew in wheat for Sichuan. J. Sichuan Agric Univ, 1997, 15: 452–456 (in Chinese with English abstract)
[3] Shi Z X, Chen X M, Line R F, Leung H, Wellings C R. Development of resistance gene analog polymorphism markers for the Yr9 gene resistance to wheat stripe rust. Genome, 2001, 44: 509–516
[4] Lei M P, Li G R, Liu C, Yang Z J. Characterization of wheat-Secale africanum introgression lines reveals evolutionary aspects of chromosome 1R in rye. Genome, 2012, 55: 765–774
[5] Yang M Y, Ren T H, Yan B J, Li Z, Ren Z L. Diversity resistance to Puccinia striiformis f. sp tritici in rye chromosome arm 1RS expressed in wheat. Genet Mol Res, 2014, 13: 8783–8793
[6] Dhaliwal A S, Mares D J, Marshall D R. Measurement of dough surface stickness associated with 1B/1R chromosome translocation in bread wheats. Cereal Sci, 1990, 12: 165–175
[7] 刘建军, 何中虎, Peña R J, 赵振东. 1BL/1RS易位对小麦加工品质的影响. 作物学报, 2004, 30: 149–153
Liu J J, He Z H, Peña R J, Zhao Z D. The effects of 1B/1R translocation on grain quality and noodle quality of bread wheat. Acta Agron Sin, 2004, 30: 149–153 (in Chinese with English abstract)
[8] Graybosh R A, Peterson C J, Hansen L E, Mattern P J. Relationships between protein solubility characteristics, 1BL/1RS, high molecular weight glutenin composition, and end-use quality in winter wheat germ plasm. Cereal Chem, 1990, 67: 342–349
[9] Lukaszewski A J. Manipulation of the 1RS?1BL translocation in wheat by induced homoeologous recombination. Crop Sci, 2000, 40: 216–225
[10] Fu S, Tang Z, Ren Z, Zhang H. Transfer to wheat (Triticum aestivum) of small chromosome segments from rye (Secale cereale) carrying disease resistance genes. J Appl Genet, 2010, 51: 115–121
[11] Scott M H, Amy A C, Gregory J H. Post-transcriptional gene silencing by double-stranded RNA. Nat Rev Genet, 2001, 2: 110–119
[12] Phillip A S, RNA interference. Genes & Develop, 2001, 15: 485–490
[13] Li J R, Zhao W, Li Q Z, Ye X G, An B Y, Li X, Zhang X S. RNA silencing of waxy gene results in low levels of amylose in the seeds of transgenic wheat (Triticum aestivum L.). Acta Genet Sin, 2005, 32: 846–854
[14] 孙重霞, 杨凤萍, 张婷, 隋晓燕, 梁荣奇, LIU Qing, 张晓东, 李保云. 利用RNAi技术抑制籽粒PPO合成改良小麦面粉白度的研究. 中国农业科学, 2013, 46: 1104–1113
Sun C X, Yang F P, Zhang T, Sui X Y, Liang R Q, Liu Q, Zhang X D, Li B Y. Down-regulation of the expression of grain ppo genes to improve wheat dough whiteness by RNA interference. Sci Agric Sin, 2013, 46: 1104–1113 (in Chinese with English abstract)
[15] Chai J F, Liu X, Jia J Z. Homoeologous cloning of ω-secalin gene family in a wheat 1BL/1RS translocation. Cell Res, 2005, 15: 658–664
[16] Murai J, Taira T, Ohta D. Isolation and characterization of the three Waxy genes encoding the granule-bound starch synthase in hexaploid wheat. Gene, 1999, 234: 71–79
[17] 张学勇醇溶蛋白电泳在小麦种质资源遗传分析中的应用. 中国农业科, 1995, 28(4): 25–32
Zhang X Y, Yang X M, Dong Y C. Genetic analysis of wheat germplasm by acid polyacrylamide gel electrophoresis of gliadins. Sci Agric Sin, 1995, 28(4): 25–32 (in Chinese with English abstract), 杨欣明, 董玉琛.
[18] 纪军, 刘冬成, 王静, 李俊明, 张爱民. 一种小麦高、低分子量麦谷蛋白亚基的提取方法. 遗传, 2008, 30: 123–126
Ji J, Liu D C, Wang J, Li J M, Zhang A M. A method of extraction and separation of wheat gluten. Hereditas (Beijing), 2008, 30: 123–126 (in Chinese with English abstract)
[19] Gil-Humanes J, Pistón F, Tollefsen S, Sollid L M, Barro F. Effective shutdown in the expression of celiac disease-related wheat gliadin T-cell epitopes by RNA interference. Proc Natl Acad Sci USA, 2010, 107: 17023–17028
[20] Hussain A, and Lukow O M. Characterization of the 1B/1R translocation in wheat using water extractable protein concentrate. Euphytica, 1994, 78: 109–113
[21] Sharma S, Bhat P R, Ehdaie B, Close T J, Lukaszewski A J, Waines J G. Integrated genetic map and genetic analysis of a region associated with root traits on the short arm of rye chromosome 1 in bread wheat. Theor Appl Genet,2009, 119: 783–793
[22] 相微微, 张怀刚, 王道文, 柳觐, 刘宝龙. 转基因小麦沉默的HMW-GS基因的遗传及表达. 麦类作物学报, 2009, 29: 185–188
Xiang W W, Zhang H G, Wang D W, Liu J, Liu B L. Inheritance and expression of silenced HMW-GS genes in wheat. J Triticeae Crops, 2009, 29: 185–188 (in Chinese with English abstract)
[23] 武茹, 高德荣, 别同德, 张晓, 赵芸, 程顺和. 转基因小麦1Dx5基因沉默的遗传表达和品质效应分析. 麦类作物学报, 2010, 30: 991–996
Wu R, Gao D R, Bie T D, Zhang X, Zhao Y, Cheng S H. Genetic analysis and quality effects of 1Dx5 subunit silent in transgenic wheat. J Triticeae Crops, 2010, 30: 991–996 (in Chinese with English abstract)
[1] MA Huan-Huan, FANG Qi-Di, DING Yuan-Hao, CHI Hua-Bin, ZHANG Xian-Long, MIN Ling. GhMADS7 positively regulates petal development in cotton [J]. Acta Agronomica Sinica, 2021, 47(5): 814-826.
[2] SU Qiang,RONG Wei,ZHANG Zeng-Yan. Cloning and functional analysis of wheat receptor-like kinase gene TaPK3A [J]. Acta Agronomica Sinica, 2019, 45(8): 1158-1165.
[3] CHENGWei,LIHe-Ping,HEShui-Lin,LIAOYu-Cai. Progress in Enhancement of Plant Resistance against Fungal Diseases through Host-Induced Gene Silencing [J]. Acta Agron Sin, 2017, 43(08): 1115-1121.
[4] WANG Sha,HE Yong,LUO Guang-Yu,YAO Min,ZHANG Xu,CHEN Xin-Bo,ZHOU Xiao-Yun. Influence of OsWR2-RNAi on Rice Cuticle Biosynthesis and Drought Resistance [J]. Acta Agron Sin, 2017, 43(03): 315-323.
[5] YANG Xiang-Dong,NIU Lu,ZHANG Wei,YANG Jing,DU Qian,XING Guo-Jie,GUO Dong-Quan,LI Qi-Yun,DONG Ying-Shan. RNAi-mediated SMV-P3 Silencing Increases Soybean Resistance to Soybean Mosaic Virus [J]. Acta Agron Sin, 2016, 42(11): 1647-1655.
[6] ZHANG Ping-Ping,MA Hong-Xiang*,YAO Jin-Bao,ZHOU Miao-Ping,ZHANG Peng. Effect of HMW-GS Deletion on Processing Quality of Soft Wheat Ningmai 9 [J]. Acta Agron Sin, 2016, 42(05): 633-640.
[7] HU Dan-Dan,ZHANG Fan,HUANG Li-Yu,ZHUO Da-Long,ZHANG Fan,ZHOU Yong-Li,SHI Ying-Yao,LI Zhi-Kang. Stress-activated Protein Kinase OsSAPK2 Involved in Regulating Resistant Response to Xanthomonas oryzae pv. oryzae in Rice [J]. Acta Agron Sin, 2015, 41(08): 1191-1200.
[8] REN Qin,WANG Ya-Jun,GUO Zhi-Hong,LI Ji-Ping,XIE Zhong-Kui,WANG Ruo-Yu,WANG Li,HUI Na-Na. Gene Silence of Phytophthora infestans Induced by Plant-mediated RNA Interference in Potato [J]. Acta Agron Sin, 2015, 41(06): 881-888.
[9] ANG Li,HUANG Yu-Lian,CHANG Ping,YAN Jun,ZHANG Ye-Lun,XIA Xian-Chun,TIAN Yu-Bing,HE Zhong-Hu,ZHANG Yong. QTL Mapping for Arabinoxylans Content and Its Relationship with Processing Quality in Common Wheat [J]. Acta Agron Sin, 2014, 40(09): 1695-1701.
[10] YE Yan-Ying,ZENG Gang,CAO Ming-Qing,MA Rong-Cai,WU Cai-Jun,YAO Lei. HC-Pro Gene Segment Mediated Hyper-Resistance to Turnip mosaic virus [J]. Acta Agron Sin, 2014, 40(03): 550-555.
[11] CHEN Xian-Chao,HUANG Li-Yu,ZHOU Yong-Li. Mechanism Analysis of Receptor-Like Kinases Gene OsBAK1L Involved in Regulating Cell Death Response of Rice [J]. Acta Agron Sin, 2013, 39(11): 1992-1999.
[12] ZHANG Jie-Qiong,LI Hong-Yan,HU Xiao-Nan,SHAN Zhi-Hui,TANG Gui-Xiang. Agrobacterium tumefaciens Mediated Transformation of RNAi CP Gene into Soybean (Glycine max L.) [J]. Acta Agron Sin, 2013, 39(09): 1594-1601.
[13] DONG Jian,YANG Hua,ZHAO Wan-Chun,LI Xiao-Yan,CHEN Qi-Jiao,GAO Xiang. Agronomic Traits and Grain Quality of Chinese Spring–Dasypyrum villosum Translocation Lines T1DL*1VS and T1DS*1VL [J]. Acta Agron Sin, 2013, 39(08): 1386-1390.
[14] ZHANG Yong,SHEN Xiao-Yong,ZHANG Wen-Xiang,CHEN Xin-Min,YAN Jun,ZHANG Yan,WANG De-Sen,WANG Zhong-Wei,LIU Yue-Fang,TIAN Yu-Bing,XIA Xian-Chun,HE Zhong-Hu. Marker-Assisted Selection of HMW-Glutenin 1Dx5+1Dy10 Gene and 1B/1R Translocation for Improving Industry Quality in Common Wheat [J]. Acta Agron Sin, 2012, 38(10): 1743-1751.
[15] WANG Jian,ZHAO Kai-Jun,QIAO Feng,YANG Sheng-Long. Genetic Effects of Different RNA Interference Fragments from OsGA20ox2 on Plant Height and Other Agronomic Traits in Rice [J]. Acta Agron Sin, 2012, 38(04): 632-638.
Full text



No Suggested Reading articles found!