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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (11): 1992-1999.doi: 10.3724/SP.J.1006.2013.01992

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Mechanism Analysis of Receptor-Like Kinases Gene OsBAK1L Involved in Regulating Cell Death Response of Rice

CHEN Xian-Chao,HUANG Li-Yu,ZHOU Yong-Li*   

  1. National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-04-01 Revised:2013-06-24 Online:2013-11-12 Published:2013-08-12
  • Contact: 周永力, E-mail: zhouyongli@caas.cn

Abstract:

Plant receptor-like kinase BAK1 (BRI1-associated kinase 1) plays an important role in Arabidopsis in regulating diverse physiological processes, including development and signals in innate immunity. In the study, we choose a homologous geneLOC_Os03g49620.4in rice and tentatively name it as OsBAK1L for structural and functional study. The result indicates that the OsBAK1L protein is a member of SERKL (Somatic embryogenesis receptor kinase Like) protein family and located in the cell membrane. OsBAK1L was down regulated in OsBAK1L-RNAi transgenic rice plants. When the rice plants were inoculated with the Xanthomonas oryzae pv. oryzicola Rs105, hypersensibility response (HR) was triggered in both the OsBAK1L-RNAi transgenic plants and the resistant 9804-Rxo1 plants, while it was postponed only in OsBAK1L-RNAi transgenic rice plants. The expression pattern of HR associated gene, OsMPK13 (Mitogen activated protein kinase 13), and HR were also delayed in transgenic plants and had the the same changing trends. These results indicate that OsBAK1L might control HR by regulating the expression of OsMPK13.

Key words: Oryza sativa, OsBAK1L, RLK, RNAi, Hypersensibility response

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