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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (11): 1992-1999.doi: 10.3724/SP.J.1006.2013.01992


Mechanism Analysis of Receptor-Like Kinases Gene OsBAK1L Involved in Regulating Cell Death Response of Rice

CHEN Xian-Chao,HUANG Li-Yu,ZHOU Yong-Li*   

  1. National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-04-01 Revised:2013-06-24 Online:2013-11-12 Published:2013-08-12
  • Contact: 周永力, E-mail: zhouyongli@caas.cn


Plant receptor-like kinase BAK1 (BRI1-associated kinase 1) plays an important role in Arabidopsis in regulating diverse physiological processes, including development and signals in innate immunity. In the study, we choose a homologous geneLOC_Os03g49620.4in rice and tentatively name it as OsBAK1L for structural and functional study. The result indicates that the OsBAK1L protein is a member of SERKL (Somatic embryogenesis receptor kinase Like) protein family and located in the cell membrane. OsBAK1L was down regulated in OsBAK1L-RNAi transgenic rice plants. When the rice plants were inoculated with the Xanthomonas oryzae pv. oryzicola Rs105, hypersensibility response (HR) was triggered in both the OsBAK1L-RNAi transgenic plants and the resistant 9804-Rxo1 plants, while it was postponed only in OsBAK1L-RNAi transgenic rice plants. The expression pattern of HR associated gene, OsMPK13 (Mitogen activated protein kinase 13), and HR were also delayed in transgenic plants and had the the same changing trends. These results indicate that OsBAK1L might control HR by regulating the expression of OsMPK13.

Key words: Oryza sativa, OsBAK1L, RLK, RNAi, Hypersensibility response

[1]Shiu S H, Bleecker A B. Plant receptor-like kinase gene family: diversity, function, and signaling. Sci STKE, 2001, 113: re22. DOI: 10.1126/scisignal.1132001re22

[2]Shiu S H, Karlowski W M, Pan R, Tzeng Y H, Mayer K F X, Li W H. Comparative analysis of the receptor-like kinase family in Arabidopsis and rice. Plant Cell, 2004, 16: 1220–1234

[3]Chinchilla D, Shan L, He P, de Vries S, Kemmerling B. One for all: the receptor-associated kinase BAK1. Trends Plant Sci, 2009, 14: 535–541

[4]Li J. Multi-tasking of somatic embryogenesis receptor-like protein kinases. Curr Opin Plant Biol, 2010, 13: 509–514

[5]Postel S, Küfner I, Beuter C, Mazzotta S, Schwedt A, Borlotti A, Halter T, Kemmerling B, Nürnberger T. The multifunctional leucine-rich repeat receptor kinase BAK1 is implicated in Arabidopsis development and immunity. Eur J Cell Biol, 2010, 89: 169–174

[6]Yang D H, Hettenhausen C, Baldwin I T, Wu J. The multifaceted function of BAK1/SERK3: plant immunity to pathogens and responses 1 to insect herbivores. Plant Signal Behav, 2011, 6: 1322–1324

[7]Ito Y, Takaya K, Kurata N. Expression of SERK family receptor-like protein kinase genes in rice. Biochim Biophys Acta, 2005, 1730: 253–258

[8]Hu H, Xiong L, Yang Y. Rice SERK1 gene positively regulates somatic embryogenesis of cultured cell and host defense response against fungal infection. Planta, 2005, 222: 107–117

[9]Singla B, Khurana J P, Khurana P. Structural characterization and expression analysis of the SERK/SERL gene family in rice (Oryza sativa). Int J Plant Genomics, 2009, DOI: 10.1155/2009/539402

[10]Li D, Wang L, Wang M, Xu, Yun Y, Luo W, Liu Y J, Xu Z H, Li J, Chong K. Engineering OsBAK1 gene as a molecular tool to improve rice architecture for high yield. Plant Biotechnol J, 2009, 7: 791–806

[11]Park H, Ryu H, Kim B, Kim S Y, Yoon I S, Nam K H. A subset of OsSERK genes, including OsBAK1, affects normal growth and leaf development of rice. Mol Cells, 2011, 32: 561–569

[12]Xu M-R(许美容). Analysis of Molecular Pathways Mediated by Nonhost Resistance Gene to Bacterial Leaf Streak and Identification of Bacterial Blight Resistant Lines and QTLs. PhD Disseration of Chinese Academy of Agricultural Sciences, 2011. pp 24–47 (in Chinese with English abstract) 

[13]Karimi M, Inzé D, Depicker A. GATEWAY vectors for Agrobacterium-mediated plant transformation. Trends Plant Sci, 2002, 7: 193–195

[14]Travella S, Keller B. Down-regulation of gene expression by RNA-induced gene silencing. In: Jones H D, Shewry P R, eds. Transgenic Wheat, Barley and Oats. New York: Humana Press, 2009. pp 185–199

[15]Hardham A. Confocal microscopy in plant–pathogen interactions. In: Bolton M D, Thomma B P H J, eds. Plant Fungal Pathogens. New York: Humana Press, 2012. pp 295–309

[16]Boller T, Felix G. A renaissance of elicitors: perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors. Annu Rev Plant Biol, 2009, 60: 379–406

[17]Shrawat A, Good A. Agrobacterium tumefaciens-mediated genetic transformation of cereals using immature embryos. In: Thorpe T A, Yeung E C, eds. Plant Embryo Culture. New York: Humana Press, 2011. pp 355–372

[18]Tang D, Wu W, Li W, Lu H, Worland A J. Mapping of QTLs conferring resistance to bacterial leaf streak in rice. Theor Appl Genet, 2000, 101: 286–291

[19]Song D, Chen J, Song F, Zheng Z. A novel rice MAPK gene, OsBIMK2, is involved in disease-resistance responses. Plant Biol, 2006, 8: 587–596

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