Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (05): 627-632.doi: 10.3724/SP.J.1006.2016.00627

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS •     Next Articles

Effect of ω-Secalin Gene Silencing on Processing Quality of Wheat 1B/1R Translocation Line

CHAI Jian-Fang*,WANG Hai-Bo*,MA Xiu-Ying,ZHANG Cui-Mian,DONG Fu-Shuang   

  1. Genetics and Physiology Institute, Hebei Academy of Agriculture and Forestry Sciences / Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, China
  • Received:2015-09-28 Revised:2016-03-02 Online:2016-05-12 Published:2016-03-11
  • Contact: 柴建芳, E-mail: chai87652130@163.com; 王海波, E-mail: nkywanghb@163.com
  • Supported by:

    This study was supported by the Natural Science Foundation of Hebei Province, China (C2014301005).

RichHTML

PDF

2342

Abstract:

Omega-secalin is an important factor for the poor processing quality of wheat 1B/1R translocation lines. In this study, an expression vector of silencing ω-secalin gene was constructed and transferred into wheat variety Jinhe 9123 via Agrobacterium tumefaciens strain EH105. The three T0 transgenic plants obtained were consecutively multiplied after PCR validation until the T4 lines. Acid-PAGE assay indicated that total amount of ω-secalins in the transgenic lines decreased 53% on average compared with that in their respective control. In the three homozygous transgenic lines, gluten index, precipitation value, and stabilization time increased significantly, whereas the agronomic traits, such as plant height, grain number per spike, 1000-grain weight and plot yield, had no significant changes compared with those in the wild type. These results suggest that silencing ω-secalin genes can improve the processing quality of wheat 1B/1R translocation lines on the premise of not affecting grain yield.

Key words: Wheat 1B, 1R translocation line, Secalin, RNAi, Gene silencing, Processing quality

[1] 周阳, 何中虎, 张改生, 夏兰琴, 陈新民, 高永超, 井赵斌, 于广军. 1BL/1RS易位系在我国小麦育种中的应用. 作物学报, 2004,30: 531–535
Zhou Y, He Z H, Zhang G S, Xia L Q, Chen X M, Gao Y C, Jing Z B, Yu G J. Utilization of 1BL/1RS translocation in wheat breeding in China. Acta Agron Sin, 2004, 30: 531–535 (in Chinese with English abstract)

[2] 杨足君, 任正隆. 抗白粉病基因Pm8在四川小麦中遗传表达初步研究. 四川农业大学学报, 1997, 15: 452–456
Yang Z J, Ren Z L. Expression of gene Pm8 for resistance to powdery mildew in wheat for Sichuan. J. Sichuan Agric Univ, 1997, 15: 452–456 (in Chinese with English abstract)
[3] Shi Z X, Chen X M, Line R F, Leung H, Wellings C R. Development of resistance gene analog polymorphism markers for the Yr9 gene resistance to wheat stripe rust. Genome, 2001, 44: 509–516
[4] Lei M P, Li G R, Liu C, Yang Z J. Characterization of wheat-Secale africanum introgression lines reveals evolutionary aspects of chromosome 1R in rye. Genome, 2012, 55: 765–774
[5] Yang M Y, Ren T H, Yan B J, Li Z, Ren Z L. Diversity resistance to Puccinia striiformis f. sp tritici in rye chromosome arm 1RS expressed in wheat. Genet Mol Res, 2014, 13: 8783–8793
[6] Dhaliwal A S, Mares D J, Marshall D R. Measurement of dough surface stickness associated with 1B/1R chromosome translocation in bread wheats. Cereal Sci, 1990, 12: 165–175
[7] 刘建军, 何中虎, Peña R J, 赵振东. 1BL/1RS易位对小麦加工品质的影响. 作物学报, 2004, 30: 149–153
Liu J J, He Z H, Peña R J, Zhao Z D. The effects of 1B/1R translocation on grain quality and noodle quality of bread wheat. Acta Agron Sin, 2004, 30: 149–153 (in Chinese with English abstract)
[8] Graybosh R A, Peterson C J, Hansen L E, Mattern P J. Relationships between protein solubility characteristics, 1BL/1RS, high molecular weight glutenin composition, and end-use quality in winter wheat germ plasm. Cereal Chem, 1990, 67: 342–349
[9] Lukaszewski A J. Manipulation of the 1RS?1BL translocation in wheat by induced homoeologous recombination. Crop Sci, 2000, 40: 216–225
[10] Fu S, Tang Z, Ren Z, Zhang H. Transfer to wheat (Triticum aestivum) of small chromosome segments from rye (Secale cereale) carrying disease resistance genes. J Appl Genet, 2010, 51: 115–121
[11] Scott M H, Amy A C, Gregory J H. Post-transcriptional gene silencing by double-stranded RNA. Nat Rev Genet, 2001, 2: 110–119
[12] Phillip A S, RNA interference. Genes & Develop, 2001, 15: 485–490
[13] Li J R, Zhao W, Li Q Z, Ye X G, An B Y, Li X, Zhang X S. RNA silencing of waxy gene results in low levels of amylose in the seeds of transgenic wheat (Triticum aestivum L.). Acta Genet Sin, 2005, 32: 846–854
[14] 孙重霞, 杨凤萍, 张婷, 隋晓燕, 梁荣奇, LIU Qing, 张晓东, 李保云. 利用RNAi技术抑制籽粒PPO合成改良小麦面粉白度的研究. 中国农业科学, 2013, 46: 1104–1113
Sun C X, Yang F P, Zhang T, Sui X Y, Liang R Q, Liu Q, Zhang X D, Li B Y. Down-regulation of the expression of grain ppo genes to improve wheat dough whiteness by RNA interference. Sci Agric Sin, 2013, 46: 1104–1113 (in Chinese with English abstract)
[15] Chai J F, Liu X, Jia J Z. Homoeologous cloning of ω-secalin gene family in a wheat 1BL/1RS translocation. Cell Res, 2005, 15: 658–664
[16] Murai J, Taira T, Ohta D. Isolation and characterization of the three Waxy genes encoding the granule-bound starch synthase in hexaploid wheat. Gene, 1999, 234: 71–79
[17] 张学勇醇溶蛋白电泳在小麦种质资源遗传分析中的应用. 中国农业科学, 1995, 28(4): 25–32
Zhang X Y, Yang X M, Dong Y C. Genetic analysis of wheat germplasm by acid polyacrylamide gel electrophoresis of gliadins. Sci Agric Sin, 1995, 28(4): 25–32 (in Chinese with English abstract), 杨欣明, 董玉琛.
[18] 纪军, 刘冬成, 王静, 李俊明, 张爱民. 一种小麦高、低分子量麦谷蛋白亚基的提取方法. 遗传, 2008, 30: 123–126
Ji J, Liu D C, Wang J, Li J M, Zhang A M. A method of extraction and separation of wheat gluten. Hereditas (Beijing), 2008, 30: 123–126 (in Chinese with English abstract)
[19] Gil-Humanes J, Pistón F, Tollefsen S, Sollid L M, Barro F. Effective shutdown in the expression of celiac disease-related wheat gliadin T-cell epitopes by RNA interference. Proc Natl Acad Sci USA, 2010, 107: 17023–17028
[20] Hussain A, and Lukow O M. Characterization of the 1B/1R translocation in wheat using water extractable protein concentrate. Euphytica, 1994, 78: 109–113
[21] Sharma S, Bhat P R, Ehdaie B, Close T J, Lukaszewski A J, Waines J G. Integrated genetic map and genetic analysis of a region associated with root traits on the short arm of rye chromosome 1 in bread wheat. Theor Appl Genet,2009, 119: 783–793
[22] 相微微, 张怀刚, 王道文, 柳觐, 刘宝龙. 转基因小麦沉默的HMW-GS基因的遗传及表达. 麦类作物学报, 2009, 29: 185–188
Xiang W W, Zhang H G, Wang D W, Liu J, Liu B L. Inheritance and expression of silenced HMW-GS genes in wheat. J Triticeae Crops, 2009, 29: 185–188 (in Chinese with English abstract)
[23] 武茹, 高德荣, 别同德, 张晓, 赵芸, 程顺和. 转基因小麦1Dx5基因沉默的遗传表达和品质效应分析. 麦类作物学报, 2010, 30: 991–996
Wu R, Gao D R, Bie T D, Zhang X, Zhao Y, Cheng S H. Genetic analysis and quality effects of 1Dx5 subunit silent in transgenic wheat. J Triticeae Crops, 2010, 30: 991–996 (in Chinese with English abstract)
[1] Zhai Sheng-Nan, Cao Xin-You, Li Hao-Sheng, Li Ji-Hu, Li Fa-Ji, Liu Jin-Dong, Xia Xian-Chun, Lyu Ying-Ying, Ma Rui-Feng, Wang Ying, Geng Hong-Wei, Liu Jian-Jun. Analysis of the genetic effects of allelic variation at the Pod-A1, Pod-D1, and Pod-2D loci on peroxidase activity in wheat grains [J]. Acta Agronomica Sinica, 2026, 52(6): 1593-1603.
[2] Song Song-Quan, Tang Cui-Fang, Liang Yu-Rong, Cheng Hong-Yan, Wang Wei-Qing. Research progress in biosynthesis and action mechanism of sRNAs and their regulation on seed development, dormancy and germination [J]. Acta Agronomica Sinica, 2026, 52(4): 959-981.
[3] WANG Yao-Kuo, WANG Wen-Zheng, ZHANG Min, LIU Xi-Wei, YANG Min, LI Hao-Yu, ZHANG Ling-Xin, YAN Yan-Fei, CAI Rui-Guo. Effects of water and nitrogen treatments on GMP synthesis and flour processing quality of winter wheat grain [J]. Acta Agronomica Sinica, 2025, 51(8): 2176-2189.
[4] DONG Zhi-Qiang, LYU Li-Hua, YAO Yan-Rong, ZHANG Jing-Ting, ZHANG Li-Hua, YAO Hai-Po, SHEN Hai-Ping, JIA Xiu-Ling. Yield and quality of strong gluten wheat Shiluan 02-1 under water and nitrogen interaction [J]. Acta Agronomica Sinica, 2023, 49(7): 1942-1953.
[5] HUANG Zhen, WU Qi-Jing, CHEN Can-Ni, WU Xia, CAO Shan, ZHANG Hui, YUE Jiao, HU Ya-Li, LUO Deng-Jie, LI Yun, LIAO Chang-Jun, LI Ru, CHEN Peng. Role of calmodulin gene (HcCaM7) and its protein acetylation is involved in kenaf response to abiotic stress [J]. Acta Agronomica Sinica, 2023, 49(2): 402-413.
[6] NING Ning, MO Jiao, HU Bing, LI Da-Shuang, LOU Hong-Xiang, WANG Chun-Yun, BAI Chen-Yang, KUAI Jie, WANG Bo, WANG Jing, XU Zheng-Hua, LI Xiao-Hua, JIA Cai-Hua, ZHOU Guang-Sheng. Comparative study on the processing quality of winter rape in different ecological zones of the Yangtze River valley [J]. Acta Agronomica Sinica, 2023, 49(12): 3315-3327.
[7] LI Ming-Jiang, LEI Jian-Feng, ZULIPIYE·Tuoheniyazi , DAI Pei-Hong, LIU Chao, LIU Xiao-Dong. Cloning and functional verification of GhIQM1 gene of cotton in response to Verticillium wilt [J]. Acta Agronomica Sinica, 2022, 48(9): 2265-2273.
[8] WANG Sha-Sha, HUANG Chao, WANG Qing-Chang, CHAO Yue-En, CHEN Feng, SUN Jian-Guo, SONG Xiao. Cloning and functional identification of TaGS2 gene related to kernel size in bread wheat [J]. Acta Agronomica Sinica, 2022, 48(8): 1926-1937.
[9] MA Huan-Huan, FANG Qi-Di, DING Yuan-Hao, CHI Hua-Bin, ZHANG Xian-Long, MIN Ling. GhMADS7 positively regulates petal development in cotton [J]. Acta Agronomica Sinica, 2021, 47(5): 814-826.
[10] SU Qiang,RONG Wei,ZHANG Zeng-Yan. Cloning and functional analysis of wheat receptor-like kinase gene TaPK3A [J]. Acta Agronomica Sinica, 2019, 45(8): 1158-1165.
[11] CHENGWei,LIHe-Ping,HEShui-Lin,LIAOYu-Cai. Progress in Enhancement of Plant Resistance against Fungal Diseases through Host-Induced Gene Silencing [J]. Acta Agron Sin, 2017, 43(08): 1115-1121.
[12] WANG Sha,HE Yong,LUO Guang-Yu,YAO Min,ZHANG Xu,CHEN Xin-Bo,ZHOU Xiao-Yun. Influence of OsWR2-RNAi on Rice Cuticle Biosynthesis and Drought Resistance [J]. Acta Agron Sin, 2017, 43(03): 315-323.
[13] YANG Xiang-Dong,NIU Lu,ZHANG Wei,YANG Jing,DU Qian,XING Guo-Jie,GUO Dong-Quan,LI Qi-Yun,DONG Ying-Shan. RNAi-mediated SMV-P3 Silencing Increases Soybean Resistance to Soybean Mosaic Virus [J]. Acta Agron Sin, 2016, 42(11): 1647-1655.
[14] ZHANG Ping-Ping,MA Hong-Xiang*,YAO Jin-Bao,ZHOU Miao-Ping,ZHANG Peng. Effect of HMW-GS Deletion on Processing Quality of Soft Wheat Ningmai 9 [J]. Acta Agron Sin, 2016, 42(05): 633-640.
[15] HU Dan-Dan,ZHANG Fan,HUANG Li-Yu,ZHUO Da-Long,ZHANG Fan,ZHOU Yong-Li,SHI Ying-Yao,LI Zhi-Kang. Stress-activated Protein Kinase OsSAPK2 Involved in Regulating Resistant Response to Xanthomonas oryzae pv. oryzae in Rice [J]. Acta Agron Sin, 2015, 41(08): 1191-1200.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd