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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (05): 627-632.doi: 10.3724/SP.J.1006.2016.00627

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Effect of ω-Secalin Gene Silencing on Processing Quality of Wheat 1B/1R Translocation Line

CHAI Jian-Fang*,WANG Hai-Bo*,MA Xiu-Ying,ZHANG Cui-Mian,DONG Fu-Shuang   

  1. Genetics and Physiology Institute, Hebei Academy of Agriculture and Forestry Sciences / Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, China
  • Received:2015-09-28 Revised:2016-03-02 Online:2016-05-12 Published:2016-03-11
  • Contact: 柴建芳, E-mail: chai87652130@163.com; 王海波, E-mail: nkywanghb@163.com
  • Supported by:

    This study was supported by the Natural Science Foundation of Hebei Province, China (C2014301005).

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Abstract:

Omega-secalin is an important factor for the poor processing quality of wheat 1B/1R translocation lines. In this study, an expression vector of silencing ω-secalin gene was constructed and transferred into wheat variety Jinhe 9123 via Agrobacterium tumefaciens strain EH105. The three T0 transgenic plants obtained were consecutively multiplied after PCR validation until the T4 lines. Acid-PAGE assay indicated that total amount of ω-secalins in the transgenic lines decreased 53% on average compared with that in their respective control. In the three homozygous transgenic lines, gluten index, precipitation value, and stabilization time increased significantly, whereas the agronomic traits, such as plant height, grain number per spike, 1000-grain weight and plot yield, had no significant changes compared with those in the wild type. These results suggest that silencing ω-secalin genes can improve the processing quality of wheat 1B/1R translocation lines on the premise of not affecting grain yield.

Key words: Wheat 1B, 1R translocation line, Secalin, RNAi, Gene silencing, Processing quality

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