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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (09): 1309-1318.doi: 10.3724/SP.J.1006.2016.01309


Mapping Quantitative Trait Loci for Seed Size and Shape Traits in Soybean

CHEN Qiang,YAN Long,DENG Ying-Ying,Xiao Er-ning,Liu Bing-Qiang,YANG Chun-Yan*,ZHANG Meng-Chen*   

  1. Sciences / Shijiazhuang Branch of National Soybean Improvement Center / Huanghuaihai Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture, Shijiazhuang 050035, China
  • Received:2016-01-26 Revised:2016-05-09 Online:2016-09-12 Published:2016-06-06
  • Contact: 张孟臣, E-mail:mengchenzhang@hotmail.net; 杨春燕, E-mail: chyyang66@163.com E-mail:chenqiangwsm@163.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31471522), the Natural Science Foundation of Heibei Province (C2015301012), the National High-tech Research and Development Program (863 Torch Program) (2012AA101106), the China Agriculture Research System (CARS-004-PS06), and the National Science and Technology Major Project (2014ZX0800402B). 


Seed size and shape not only relate to seed yield and quality, but also affect mechanical seeding in soybean (Glycine max L.). In this study, F6:8 and F6:9 populations derived from Jidou 12 × Heidou were used to analyze the genetic character and detect quantitative trait locus (QTL) for seed length, seed width, seed thickness, seed length-to-width ratio, seed length-to-thickness ratio, and seed width-to-thickness ratio. Softwares WinQTLCart 2.5, QTLNetwork 2.1 and IciMapping 4.1 were used to identify the additive, epistatic and environmentally interacted QTLs for seed size and shape related traits. As results, the heritability of the six traits varied from 64.01% to 79.57%. A total of 38 additive QTLs were identified to be located on 12 chromosomes, with the heritability varying from 2.21% to 10.71%.Eight of them(qSL-17-1,qSL-18-1, qSW-6-1, qST-2-1, qST-6-1, qSLT-2-2, qSWT-2-1,and qSWT-20-1)were identified using three methods, simultaneously. In the meantime, seven pairs of additive × additive epistasis were detected and the heritability of epistasis pairs ranged from 0.78 to 6.20%. Additionally, the effects of QTL by environment interaction ranged from 0.0005% to 0.3900%. The QTL identified using different mapping softwares in this study could provide a reliable theoretical basis for marker-assisted selection breeding.

Key words: Soybean, Seed shape, Quantitative trait locus, Epistasis

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