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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (05): 669-677.doi: 10.3724/SP.J.1006.2017.00669


Molecular Regulation and Substance Exchange Dynamics at Dormancy and Budbreak Stages in Overwintering Buds of Tea Plant

TANG Hu1,2,**,HAO Xin-Yuan2,**,WANG Lu2,XIAO Bin1,WANG Xin-Chao2,*,YANG Ya-Jun1,2,*   

  1. 1 College of Horticulture, Northwest A&F University, Yangling 712100, China; 2 Tea Research Institute of Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China
  • Received:2016-10-17 Revised:2017-01-21 Online:2017-05-12 Published:2017-02-17
  • Contact: Wang Xinchao, E-mail: xcw75@tricaas.com, Tel: 0571-86653162 ; Yang Yajun: Email: yjyang@tricaas.com E-mail:tanghu@tricaas.com
  • Supported by:

    This study was supported by the National Science Foundation of China (31370690), the Earmarked Fund for China Agriculture Research System (CARS-23), and the Chinese Academy of Agricultural Sciences through an Innovation Project for Agricultural Sciences and Technology (CAAS-ASTIP-2014-TRICAAS).


Early sprouting cultivar Longjing 43 and later sprouting cultivar Biyun were employed in this study. The levels of substance exchange were monitored by detecting the fluorescence signal in calcein treated overwintering buds. The glucanase related genes were identified by sequence homology analysis. Their characteristics and expression patterns during different time of winter were further analyzed. The substance exchanges were detected either in stem-bud unit or mother leaf-stem unit. From the initial formation to release in dormancy, the substance exchange in overwintering buds showed strong-weak-strong variation patterns in both test cultivars, however, the duration of weak exchange stage was much shorter in Longjing 43 than in Biyun. Moreover, there was a close correlation between substance exchange variation pattern and the expression pattern of CsGLU1, a gene identified in tea plant with positive callose hydrolyzation activity. On the basis of promoter sequence analysis, plenty of transcription factor binding sequences related to hormone signaling, cold stimulation and dormancy regulation were found in CsGLU1 promoter region, which validates its putative functions in dormancy regulation. In conclusion, overwintering buds of tea plant have substance exchange with stem and mother leaf both in dormancy and non-dormancy status, furthermore, the variation of substance exchange level was consistent to the changes of dormancy status. CsGLU1 is a callose hydrolyzation related gene, which is supposed to be a key gene regulating tea plant dormancy transition through affecting the substance exchange in overwintering buds. The study provides meaningful results for understanding the changes of dormancy statuses in overwintering buds and deeply exploring the regulation mechanism in tea plant with different sprouting phenophase.

Key words: Tea plant, Overwinter bud dormancy, Substances exchange, Calcein

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