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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (07): 1012-1020.doi: 10.3724/SP.J.1006.2017.01012


Positive Regulation of CsbZIP4 Transcription Factor on Salt Stress Response in Transgenic Arabidopsis

CAO Hong-Li1,2,**,WANG Lu1,**,QIAN Wen-Jun1,HAO Xin-Yuan1,YANG Ya-Jun1,*,WANG Xin-Chao1,*   

  1. 1Tea Research Institute, Chinese Academy of Agricultural Sciences / National Center for Tea Improvement / Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China; 2College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2016-10-21 Revised:2017-03-01 Online:2017-07-12 Published:2017-03-24
  • Contact: Wang Xinchao, E-mail: xcw75@tricaas.com, Tel: 0571-86653162;Yang Yajun, E-mail: yjyang@tricaas.com. E-mail:lili9885@126.com
  • Supported by:

    This study was supported by the Natural Science Foundation of Zhejiang Province (LY14C160001), the National Natural Science Foundation of China (31500564), the Earmarked Fund for China Agriculture Research System (CARS-23), and the Chinese Academy of Agricultural Sciences through an Innovation Project for Agricultural Sciences and Technology (CAAS-ASTIP-2014-TRICAAS).


Basic region / leucine zipper (bZIP) transcription factors is a multi-functional protein family in eukaryotes, which is involved in various biological processes including seed maturation, light signaling regulation and stress response. According to sequence similarity and conserved motifs, the AtbZIPs are classified into 10 groups (A, B, C, D, E, F, G, H, I, and S). In this study, CsbZIP4 of C-group bZIP transcription factors was as an objective, the expression patterns of CsbZIP4 in response to abiotic stress in tea plants were investigated, and the salinity tolerance caused by CsbZIP4 overexpression in Arabidopsis was analyzed. The CsbZIP4 from tea plant leaves was up-regulated under 4°C, exogenous ABA, salinity and dehydration stresses, especially induction of salinity and dehydration stresses led to up-regulation of 2.9-fold and 2.2-fold, respectively. In roots, CsbZIP4 was down-regulated in response to cold, salinity and dehydration stresses, especially down-regulated by 2-fold under salinity stress. Under fluorescence microscope, it was indicated that CsbZIP4 was located in nucleus. Constitutive overexpression of CsbZIP4 in transgenic Arabidopsis lowered the plants’ sensitivity to exogenous ABA and salinity at germination stage. Moreover, CsbZIP4 overexpression lines exhibited higher salinity tolerance under 300 mmol L–1 NaCl conditions and higher SPAD values. AtSOS1, which is a salinity responsive gene, was strongly induced in overexpression lines. In conclusion, CsbZIP4 positively regulates salt stress response in Arabidopsis, and might be closely related to the tolerance to salinity stress in tea plant.

Key words: Tea plant, CsbZIP4 transcription factor, Abiotic stress, Salinity tolerance

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