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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (07): 1003-1011.doi: 10.3724/SP.J.1006.2017.01003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

QTL Mapping for Oil, Protein and Sesamin Contents in Seeds of White Sesame

WU Kun1,2,WU Wen-Xiong1,YANG Min-Min1,LIU Hong-Yan1,HAO Guo-Cun1,ZHAO Ying-Zhong1,*   

  1. 1 Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China; 2 Biotechnology Research Center of Southwest University, Chongqing 400715, China
  • Received:2016-08-18 Revised:2017-01-20 Online:2017-07-12 Published:2017-03-22
  • Contact: 赵应忠, E-mail: zhaoyz63@163.com, Tel: 027-86733625 E-mail:adwukun@126.com
  • Supported by:

    The work was supported by the National Natural Science Foundation of China (31201243), the National Key Basic Research Program of China (973 Program) (2011CB109304), and the China Agriculture Research System (CARS-15).

Abstract:

Seed oil content, protein content and sesamin content are three important targets for sesame breeding. The objective of this study was to explore the genetic model and detect quantitative trait loci (QTLs) for three quality traits using a mapping population containing 224 recombinant inbred lines (RILs, F9). The three quality traits of seeds were measured by near infrared reflectance spectroscopy (NIRS) in three environments. Correlation analysis showed oil content was negatively correlated with protein content but positively correlated with sesamin content, whereas, protein content was negatively correlated with sesamin content. Using mixed composite interval mapping (MCIM) method, a total of eight QTLs distributed were detected for the three seed quality traits, with contribution ratio of QTL additive effect from 0.41% to 14.55%. While 13 QTLs were detected on nine LGs with multiple interval mapping (MIM), explaining 5.2% to 18.6% of the total phenotypic variation. Five QTLs were detected to be located on the same regions with MCIM and MIM, and two QTLs were detected in more than two environments. The Qoc-5 and Qsc-5 were located on the same region of LG5, both of which had positive additive effect, while the Qpc-5 was on the nearby region with negative additive effect. Similar results also existed on LG2 and LG1, which might be due to pleiotropism or closely linkage. Accordingly, the contents of oil and sesamin in seed can be improved together, while select protein content negatively in breeding.

Key words: Sesame, Oil content, Protein content, Sesamin content, QTL

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