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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (07): 993-1002.doi: 10.3724/SP.J.1006.2017.00993

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Screening of Tobacco Genotypes with Tolerance to Low-Nitrogen and Analysis of Their Nitrogen Efficiency

ZHONG Si-Rong1,CHEN Ren-Xiao2,TAO Yao1,GONG Si-Yu1,HE Kuan-Xin2,ZHANG Qi-Ming2,ZHANG Shi-Chuan1,LIU Qi-Yuan1,*   

  1. 1 Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / Key Laboratory of Crop Physiology, Ecology and Genetic Breeding of Jiangxi Province / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China; 2 Jiangxi Leaf Tobacco Research Institute, Nanchang 330045, China
  • Received:2016-11-09 Revised:2017-01-21 Online:2017-07-12 Published:2017-02-17
  • Contact: 刘齐元, E-mail: qiyuanl@126.com
  • Supported by:

    This study was supported by the Jiangxi Province Tobacco Monopoly Bureau of Science and Technology Projects (201401001).

Abstract:

It is an effective way to screen tobacco genotypes with tolerance to low-nitrogen which increase nitrogen use efficiency and decreased nitrogen pollution. In this paper, 74 tobacco genotypes were treated with low nitrogen (0.5 mmol L–1) and normal nitrogen (5.0 mmol L–1) levels in hydroponics at seedlings stage, and evaluated and screened by using descriptive statistics, factor analysis and cluster analysis The variation coefficient of root volume, root biomass, stalk and leaf nitrogen accumulation, aboveground biomass were relatively large under low nitrogen and normal nitrogen conditions, with rang of 0.37–0.68 and 0.38–0.64, respectively. The principal components were similar for both treatments; the stalk and leaf nitrogen accumulation and aboveground biomass played a major role. According to heatmap cluster analysis and scatter diagram analysis, 15 genotypes with tolerance to low-nitrogen were screened out, accounting for 20.3% of the test materials, eight of which were low nitrogen high efficiency and normal nitrogen low efficiency genotypes, accounting for 53.3% of the low-nitrogen tolerant genotypes, six were low nitrogen low efficiency and normal nitrogen low efficiency genotypes, accounting for 40.0%, and one was low nitrogen high efficiency and normal nitrogen high efficiency, accounting for 0.7%. Eight low-nitrogen sensitive genotypes were screened out, among them six belonged to low nitrogen low efficiency and normal nitrogen high efficiency, accounting for 75.0%, two to low nitrogen low efficiency and normal nitrogen low efficiency, accounting for 25.0%. The study suggested that 14P9 was low-nitrogen tolerance and nitrogen high efficiency genotype, while Zhongyan 100 and K394 were low-nitrogen sensitive and nitrogen low efficiency genotypes.

Key words: Tobacco, Genotype, Seedling stage, Nitrogen, High nitrogen efficiency

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