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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (07): 993-1002.doi: 10.3724/SP.J.1006.2017.00993

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Screening of Tobacco Genotypes with Tolerance to Low-Nitrogen and Analysis of Their Nitrogen Efficiency

ZHONG Si-Rong1,CHEN Ren-Xiao2,TAO Yao1,GONG Si-Yu1,HE Kuan-Xin2,ZHANG Qi-Ming2,ZHANG Shi-Chuan1,LIU Qi-Yuan1,*   

  1. 1 Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education / Key Laboratory of Crop Physiology, Ecology and Genetic Breeding of Jiangxi Province / College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China; 2 Jiangxi Leaf Tobacco Research Institute, Nanchang 330045, China
  • Received:2016-11-09 Revised:2017-01-21 Online:2017-07-12 Published:2017-02-17
  • Contact: 刘齐元, E-mail: qiyuanl@126.com
  • Supported by:

    This study was supported by the Jiangxi Province Tobacco Monopoly Bureau of Science and Technology Projects (201401001).

Abstract:

It is an effective way to screen tobacco genotypes with tolerance to low-nitrogen which increase nitrogen use efficiency and decreased nitrogen pollution. In this paper, 74 tobacco genotypes were treated with low nitrogen (0.5 mmol L–1) and normal nitrogen (5.0 mmol L–1) levels in hydroponics at seedlings stage, and evaluated and screened by using descriptive statistics, factor analysis and cluster analysis The variation coefficient of root volume, root biomass, stalk and leaf nitrogen accumulation, aboveground biomass were relatively large under low nitrogen and normal nitrogen conditions, with rang of 0.37–0.68 and 0.38–0.64, respectively. The principal components were similar for both treatments; the stalk and leaf nitrogen accumulation and aboveground biomass played a major role. According to heatmap cluster analysis and scatter diagram analysis, 15 genotypes with tolerance to low-nitrogen were screened out, accounting for 20.3% of the test materials, eight of which were low nitrogen high efficiency and normal nitrogen low efficiency genotypes, accounting for 53.3% of the low-nitrogen tolerant genotypes, six were low nitrogen low efficiency and normal nitrogen low efficiency genotypes, accounting for 40.0%, and one was low nitrogen high efficiency and normal nitrogen high efficiency, accounting for 0.7%. Eight low-nitrogen sensitive genotypes were screened out, among them six belonged to low nitrogen low efficiency and normal nitrogen high efficiency, accounting for 75.0%, two to low nitrogen low efficiency and normal nitrogen low efficiency, accounting for 25.0%. The study suggested that 14P9 was low-nitrogen tolerance and nitrogen high efficiency genotype, while Zhongyan 100 and K394 were low-nitrogen sensitive and nitrogen low efficiency genotypes.

Key words: Tobacco, Genotype, Seedling stage, Nitrogen, High nitrogen efficiency

[1] Sreeramamuthy C H, Arishukumar P H, Nageswararao C R. Change in concentration of nitrogenous constituents in flue-cured tobacco leaf as affected by nitrogen fertilization in Vertisols. Tob Res, 1996, 22: 22–25 [2] Drake M P, Vann M C, Fisher L R. Influence of nitrogen application rate on the yield, quality, and chemical components of flue-cured tobacco, part II: Application method. Tob Sci, 2015, 52: 26–34 [3] 梁景霞, 梁康迳, 祁建民, 林文雄, 陈顺辉, 丘贵盛. 烟草不同基因型耐低氮能力差异评价. 植物遗传资源学报, 2007, 8: 451–455 Liang J X, Liang K J, Qi J M, Lin W X, Chen S H, Qiu G S. Evaluation of low nitrogen tolerance for different tobacco varieties. J Plant Genet Resour, 2007, 8: 451–455 (in Chinese with English abstract) [4] 巨晓棠, 谷保静. 我国农田氮肥施用现状、问题及趋势. 植物营养与肥料学报, 2014, 20: 783–795 Ju X T, Gu B J. Status-quo, problem and trend of nitrogen fertilization in China. J Plant Nutr Fert, 2014, 20: 783–795 (in Chinese with English abstract) [5] 黄元炯, 张生杰, 马永建, 杨铁钊. 不同烤烟品种(基因型)氮效率及耐低氮能力的差异. 烟草科技, 2013, (4): 71–77 Huang Y J, Zhang S J, Ma Y J, Yang T Z. Differential analysis of nitrogen utilization and tolerance to low nitrogen level between flue-cured tobacco cultivars (genotypes). Tob Sci Tech, 2013, (4): 71–77 (in Chinese with English abstract) [6] 刘辉, 赵竹青. 植物氮营养高效基因型筛选指标研究进展. 安徽农业科学, 2006, 34: 3265–3267 Liu H, Zhao Z Q. Research progress in the selecting standard of the plant genotype with nitrogen efficiency. J Anhui Agric Sci, 2006, 34: 3265–3267 (in Chinese with English abstract) [7] 王进军, 黄瑞冬. 玉米氮效率及其研究进展. 玉米科学, 2005, 13(1): 89–92 Wang J J, Huang R D. Nitrogen use efficiency and its research advances in maize. J Maize Sci, 2005, 13(1): 89–92 (in Chinese with English abstract) [8] 陈范骏, 米国华, 春 亮, 刘建安, 王 艳, 张福锁. 玉米氮效率的杂种优势分析. 作物学报, 2004, 30: 1014–1018 Chen F J, Mi G H, Chun L, Liu J A, Wang Y, Zhang F S. Analysis of heterosis for nitrogen use efficiency in maize. Acta Agron Sin, 2004, 30: 1014–1018 (in Chinese with English abstract) [9] 徐福荣, 汤翠凤, 余藤琼, 严红梅, 周海, 李俊, 蒋会兵, 叶昌荣, 戴陆园. 利用叶绿素仪SPAD值筛选耐低氮水稻种质. 分子植物育种, 2005, 3: 695–700 Xu F R, Tang C F, Yu T Q, Yan H M, Zhou H, Li J, Jiang H B, Ye C R, Dai L Y. Screening of rice germplasms for tolerance to low-nitrogen using SPAD-value by chlorophyll meter. Mol Plant Breed, 2005, 3: 695–700 (in Chinese with English abstract) [10] 黄高宝, 张恩和, 胡恒觉. 不同玉米品种氮素营养效率差异的生态生理机制. 植物营养与肥料学报, 2001, 7: 293–297 Huang G B, Zhang E H, Hu H J. Eco-physiological mechanism on nitrogen use efficiencydifference of corn varieties. J Plant Nutr Fert, 2001, 7: 293–297 (in Chinese with English abstract) [11] Machado A T, Magalhaes J R, Magnavaca R, Silva M R. Enzyme activities involved with nitrogen metabolism in different maize genotypes. Rev Bras Fisiol Veg, 1992, 4: 45–47 [12] Muchow R C. Effect of nitrogen supply on the comparative productivity of maize and sorghum in a semi-arid tropical environment III Grain yield and nitrogen accumulation. Field Crops Res, 1988, 18: 31–43 [13] 向春阳, 常强, 马兴林, 关义新, 凌碧莹, 张宝石. 玉米不同基因型对氮营养胁迫的反应. 黑龙江八一农垦大学学报, 2002, 14(4): 5–7 Xiang Y C, Chang Q, Ma X L, Guan Y X, Ling B X, Zhang B S. Response to nitrogen stress on maize genotypes. J Heilongjiang Aug First land Reclam Univ, 2002, 14(4): 5–7 (in Chinese with English abstract) [14] 梁景霞, 梁康迳, 林文雄, 祁建民, 陈顺辉, 丘贵盛. 烟草氮素营养的基因型差异初探. 中国烟草学报, 2007, 13(6): 36–40 Liang J X, Liang K J, Lin W X, Qi J M, Chen S G, Qiu G S. Primary study of genotypic differences in nitrogen nutrition in tobacco germplasms. Acta Tab Sin, 2007, 13(6): 36–40 (in Chinese with English abstract) [15] 杨中义, 张发明, 李永智, 肖志新, 李永亮, 刘莉莉, 宋鹏飞, 周冀衡. 不同烤烟品种对氮素胁迫响应差异的研究. 云南农业大学学报(自然科学版), 2011, 26: 240–245 Yang Z Y, Zhang F M, Li Y Z, Xiao Z X, Li Y L, Liu L L, Song P F, Zhou J H. Evaluation of low nitrogen stress for different tobacco varieties. J Yunnan Agric Univ(Nat Sci), 2011, 26: 240–245 (in Chinese with English abstract) [16] 刘巧真, 郭芳阳, 梁涛, 曹华民, 吴照辉, 闫小毛, 陈廷贵. 幼苗期不同烤烟品种对氮营养响应的差异研究. 湖北农业科学, 2015, 54: 2950–2953 Liu Q Z, Guo F Y, Liang T, Cao H M, Wu Z H, Yan X M, Chen T G. Study on response of different flue-cured tobacco to nitrogen nutrition at seedling stage. Hubei Agric Sci, 2015, 54: 2950–2953 (in Chinese with English abstract) [17] 韩璐, 张薇. 棉花苗期氮营养高效品种筛选. 中国农学通报, 2011, 27(1): 84–88 Han L, Zhang W. Screening of cotton varieties with high nitrogen efficiency at seedling stage. Chin Agric Bull, 2011, 27(1): 84–88 (in Chinese with English abstract) [18] 史宏志, 邸慧慧, 赵晓丹, 刘国顺, 马永建, 王维超, 段杰. 豫中烤烟烟碱和总氮含量与中性香气成分含量的关系. 作物学报, 2009, 35: 1299–1305 Shi H Z, Di H H, Zhao X D, Liu G S, Ma Y J, Wang W C, Duan J. Relationship of nicotine and total nitrogen contents with neutral aroma components in flue-cured tobacco in central area of Henan province. Acta Agron Sin, 2009, 35: 1299–1305 (in Chinese with English abstract) [19] 陈晨, 龚海青, 张敬智, 徐寓军, 郜红建. 不同基因型水稻苗期氮营养特性差异及综合评价. 中国生态农业学报, 2016, 24: 1347–1355 Chen C, Gong H Q, Zhang J Z, Xu Y J, Gao H J. Nitrogen nutrition characteristics and comprehensive evaluation on different rice cultivars at the seedling stage. Chin J Eco-Agric, 2016, 24: 1347–1355 (in Chinese with English abstract) [20] 陈文荣, 曾玮玮, 李云霞, 李永强, 郭卫东. 高丛蓝莓对干旱胁迫的生理响应及其抗旱性综合评价. 园艺学报, 2012, 39: 637–646 Chen W R, Zeng W W, Li Y X, Li Y Q, Guo W D. The physiological responds of highbush blueberry to drought stress and the comprehensive evaluation on their drought resistance capacity. Acta Hort Sin, 2012, 39: 637–646 (in Chinese with English abstract) [21] 陈二影, 杨延兵, 秦岭, 张华文, 刘宾, 王海莲, 陈桂玲, 于淑婷, 管延安. 谷子苗期氮高效品种筛选及相关特性分析. 中国农业科学, 2016, 49: 3287–3297 Chen E Y, Yang Y B, Qin L, Zhang H W, Liu B, Wang H L, Chen G L, Yu S T, Guan Y A. Evaluation of nitrogen efficient cultivars of foxtail millet and analysis of the related characters at seedling stage. Sci Agric Sin, 2016, 49: 3287–3297 (in Chinese with English abstract) [22] 黄永兰, 黎毛毛, 芦明, 万建林, 龙起樟, 王会民, 唐秀英, 范志洁. 氮高效水稻种质资源筛选及相关特性分析. 植物遗传资源学报, 2015, 16: 87–93 Huang Y L, Li M M, Lu M, Wang J L, Long Q Z, Wang H M, Tang X Y, Fan Z H. Selection of rice germplasm with high nitrogen utilization efficiency and its analysis of the related characters. J Plant Genet Resour, 2015, 16: 87–93 (in Chinese with English abstract) [23] Robinson N, Fletcher A, Whan A, Critchley C, von Wirén N, Lakshmanan P, Schmidt S. Sugarcane genotypes differ in internal nitrogen use efficiency. Funct Plant Biol, 2007, 34: 1122–1129 [24] Singh U, Ladha J K, Castillo E G, Punzalan G, Tirol-Padre A. Genotypic variation in nitrogen use efficiency in medium and long-duration rice. Field Crops Res, 1998, 58: 35–53 [25] 李敏, 张洪程, 杨雄, 葛梦婕, 马群, 魏海燕, 戴其根, 霍中洋, 许轲, 曹利强, 吴浩. 水稻高产氮高效型品种的根系形态生理特征. 作物学报, 2012, 38: 648–656 Li M, Zhang H C, Yang X, Ge M J, Ma Q, Wei H Y, Dai Q G, Huo Z Y, Xu K, Cao L Q, Wu H. Root morphological and physiological characteristics of rice cultivars with high yield and high nitrogen use efficiency. Acta Agron Sin, 2012, 38: 648–656 (in Chinese with English abstract) [26] 李淑文, 周彦珍, 文宏达, 李雁鸣, 肖凯. 不同小麦品种氮效率和产量性状的研究. 植物遗传资源学报, 2006, 7: 204–208 Li S W, Zhou Y Z, Wen H D, Li Y M, Xiao K. Nitrogen use efficiency and yield traits in different wheat varieties. J Plant Genet Resour, 2006, 7: 204–208(in Chinese with English abstract) [27] 李强, 罗延宏, 谭杰, 孔凡磊, 杨世民, 袁继超. 玉米杂交种苗期耐低氮指标的筛选与综合评价. 中国生态农业学报, 2014, 22: 1190–1199 Li Q, Luo Y H, Tan J, Kong F L, Yang S M, Yuan J C. Indexes screening and comprehensive evaluation of low nitrogen tolerance of hybrid maize cultivars at seedling stage. Chin J Eco-Agric, 2014, 22: 1190–1199 (in Chinese with English abstract) [28] 姜爽, 吴凤芝, 关颂娜, 于敏锐. 耐低氮胁迫黄瓜品种的筛选. 中国蔬菜, 2012, (8): 51–56 Jiang S, Wu F Z, Guan S N, Yu M R. Screening of cucumber cultivars for tolerance to low nitrogen stress. China Veget, 2012, (8): 51–56 (in Chinese with English abstract) [29] Eghball B, Maranville J W. Root development and nitrogen influx of corn genotypes grown under combined drought and nitrogen stress. Agron J, 1993, 85: 147–152 [30] 赵化田, 王瑞芳, 许云峰, 安调过. 小麦苗期耐低氮基因型的筛选与评价. 中国生态农业学报, 2011, 19: 1199–1204 Zhao H T, Wang R F, Xu Y F, An D G. Screening and evaluating low nitrogen tolerant wheat genotype at seedling stage. Chin J Eco-Agric, 2011, 19: 1199–1204 (in Chinese with English abstract) [31] 王艳, 米国华, 陈范骏, 张福锁. 玉米氮素吸收的基因型差异及其与根系形态的相关性. 生态学报, 2003, 23: 297–302 Wang Y, Mi G H, Chen F J, Zhang F S. Genotypic differences in nitrogen uptake by maize inbred lines its relation to root morphology. Acta Ecol Sin, 2003, 23: 297–302 (in Chinese with English abstract) [32] 春亮, 陈范骏, 张福锁, 米国华. 不同氮效率玉米杂交种的根系生长、氮素吸收与产量形成. 植物营养与肥料学报, 2005, 11: 615–619 Chun L, Chen F J, Zhang F S, Mi G H. Root growth, nitrogen uptake and yield formation of hybrid maize with different N efficiency. J Plant Nutr Fert, 2005, 11: 615–619 (in Chinese with English abstract) [33] 裴雪霞, 王姣爱, 党建友, 张定一. 耐低氮小麦基因型筛选指标的研究. 植物营养与肥料学报, 2007, 13: 93–98 Pei X X, Wang J A, Dang J Y, Zhang D Y. An approach to the screening index for low nitrogen tolerant wheat genotype. J Plant Nutr Fert, 2007, 13: 93–98 (in Chinese with English abstract) [34] 张锡洲, 阳显斌, 李廷轩, 余海英. 小麦氮素利用效率的基因型差异. 应用生态学报, 2011, 22: 369–375 Zhang X Z, Yang X B, Li Y X, Yu H Y. Genotype difference in nitrogen utilization efficiency of wheat. Chin J Appl Ecol, 2011, 22: 369–375 (in Chinese with English abstract) [35] 崔文芳, 高聚林, 王志刚, 崔超, 胡树平, 于晓芳, 孙继颖, 苏治军. 玉米自交系氮效率基因型差异分析. 玉米科学, 2013, 21(3): 6–12 Cui W F, Gao J L, Wang Z G, Cui C, Hu S P, Yu X F, Sun J Y, Su Z J. Analysis on genotypic difference in nitrogen efficiency of maize inbred lines. J Maize Sci, 2013, 21(3): 6–12 (in Chinese with English abstract) [36] 程建峰, 戴廷波, 曹卫星, 姜东, 刘宜柏. 不同类型水稻种质氮素营养效率的变异分析. 植物营养与肥料学报, 2007, 13: 175–183 Cheng J F, Dai T B, Cao W X, Jiang D, Liu Y B. Variations of nitrogen nutrition efficiency in different rice germplasmtypes. J Plant Nutr Fert, 2007, 13: 175–183 (in Chinese with English abstract) [37] 李群平, 王素琴, 王海涛, 叶红朝, 张函. 洛阳烟区烤烟新品种中烟100、中烟101的生产适应性研究. 中国烟草科学, 2005, (1): 10–13 Li Q P, Wang S Q, Wang H T, Ye H C, Zhang H. Production adaptability of new flue-cured variety Zhongyan 100 and Zhongyan 101 in Luoyang. Chin Tob Sci, 2005, (1): 10–13 (in Chinese with English abstract) [38] 周金仙. 不同烤烟品种生态适应性评价. 种子, 2006, 25(2): 58–60 Zhou J X. Different flue-cured tobacco varieties ecological adaptability evaluation. Seed, 2006, 25(2): 58–60 (in Chinese)

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