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Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (2): 292-303.doi: 10.3724/SP.J.1006.2022.12013

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Phenotypic and genetic analyses of a rice mutant eed1 with defected embryo and endosperm development

YANG Jin1,2(), BAI Ai-Ning3, BAI Xue3, CHEN Juan3, GUO Lin1, LIU Chun-Ming1,3,*()   

  1. 1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    2Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    3Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
  • Received:2021-02-18 Accepted:2021-04-26 Online:2022-02-12 Published:2021-05-17
  • Contact: LIU Chun-Ming E-mail:jinyang201587@163.com;liuchunming@caas.cn
  • Supported by:
    This study was supported by the Beijing Scientific and Technological Research Program(Z181100002418010);the CAS-Commonwealth Scientific and Industrial Research Organization Bilateral Collaboration Project(151111KYSB20180049);the National Major Project for Developing New GM Crops(2019ZX08010-001)

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Abstract:

A stably inherited embryo and endosperm defective mutant, named embryo and endosperm defective 1 (eed1), was obtained from the mutant population of rice Zhonghua 11, generated by ethylmethane sulfonate (EMS). The 1000-grain weight, grain length, grain width, grain thickness, germination rate, total starch, amylose, and storage protein contents in eed1 mature caryopses were significantly decreased compared with wild type. In eed1, the caryopses were shrunken and the endosperm was opaque. Scanning electron microscopy observation revealed that starch granules in eed1 endosperm cells were loosely packed, in single, disperse and spherical forms. The embryo of eed1 was abnormal, and some caryopses showed no sign of embryonic differentiation. Most genes involved in biosynthesis of starch and storage protein were down-regulated in eed1 endosperm by qRT-PCR. EED1 was mapped in an interval of 672 kb on chromosome 9 using a F2 population derived from a cross between eed1 and Nanjing 6. The region contained 114 open reading frames. This study lays a foundation for further studying EED1 gene in regulating development of rice embryo and endosperm.

Key words: rice, embryo and endosperm development, grain filling

Table 1

Primers for qRT-PCR used in this study"

基因名
Genes ID		 正向引物序列
Forward sequences (5'-3')		 反向引物序列
Reverse sequences (5'-3')
Ubiquitin CTGTCAACTGCCGCAAGAAG GGCGAGTGACGCTCTAGTTC
OsAGPL1 GGAAGACGGATGATCGAGAAAG CACATGAGATGCACCAACGA
OsAGPL2 AGTTCGATTCAAGACGGATAGC CGACTTCCACAGGCAGCTTATT
OsAGPL3 AAGCCAGCCATGACCATTTG CACACGGTAGATTCACGAGACAA
OsAGPS1 GTGCCACTTAAAGGCACCATT CCCACATTTCAGACACGGTTT
OsAGPS2a ACTCCAAGAGCTCGCAGACC GCCTGTAGTTGGCACCCAGA
OsAGPS2b AACAATCGAAGCGCGAGAAA GCCTGTAGTTGGCACCCAGA
OsSS I GGGCCTTCATGGATCAACC CCGCTTCAAGCATCCTCATC
OsS IIa GCTTCCGGTTTGTGTGTTCA CTTAATACTCCCTCAACTCCACCAT
OsSS IIc GACCGAAATGCCTTTTTCTCG GGGCTTGGAGCCTCTCCTTA
OsSS IIIa GCCTGCCCTGGACTACATTG GCAAACATATGTACACGGTTCTGG
OsSS IIIb ATTCCGCTCGCAAGAACTGA CAACCGCAGGATAACGGAAA
OsSS IVa GGGAGCGGCTCAAACATAAA CCGTGCACTGACTGCAAAAT
OsSS IVb ATGCAGGAAGCCGAGATGTT ACGACAATGGGTGCCAAGAT
OsGBSS I
OsGBSS II		 AACGTGGCTGCTCCTTGAA
AGGCATCGAGGGTGAGGAG		 TTGGCAATAAGCCACACACA
CCATCTGGCCCACATCTCTA
OsBE I
OsBE IIa		 TGGCCATGGAAGAGTTGGC
GCCAATGCCAGGAAGATGA		 CAGAAGCAACTGCTCCACC
GCGCAACATAGGATGGGTTT
OsBE IIb ATGCTAGAGTTTGACCGC AGTGTGATGGATCCTGCC
OsISA1 TGCTCAGCTACTCCTCCATCATC AGGACCGCACAACTTCAACATA
OsISA2 TAGAGGTCCTCTTGGAGG AATCAGCTTCTGAGTCACCG
OsISA3 ACAGCTTGAGACACTGGGTTGAG GCATCAAGAGGACAACCATCTG
OsPUL ACCTTTCTTCCATGCTGG CAAAGGTCTGAAAGATGGG
OsDPE2 CAAGTACACCACAAGACCAGCAA CGTCCAACAGCGAATCCAAT
OsPHOL TTGGCAGGAAGGTTTCGCT CGAAGCCTGAAGTGAACTTGCT
OsPHOH CACCAAGACGAAGCTCATCAAG TTCACTCGTTGCTGGGTTCTC
GluA1 CATTTGAGCCAATTCGGAGT GGCCTGATTGTTGGAACTGT
GluA2 GCAAGAGCAGGAACAAGGAC CCTCATGGTGCAAAAGGTCT
GluA3 TGAAAACCAACCCTGACTCC ACTCATCTCCCCTGTTGTGC
GluB1 GCCAAAGTCAGAGCCAAAAG GAACCAATGTGCAACACCAG
GluB4 GCGACCAGAAGGCTACAAAG TTGCTTGTTGATCGTTGCTC
Glutelinλ TGGCGACCATAGCTTTCTCT GGGTTGTGCCATGGATTTAC
Globulin1 ATCGAGAACGGCGAGAAGT GGACGGAGATGGTATGGAGA
Globulin2 CGACGAGGTGTTCTACGTCA GTGTTGGCGGAGTAGACGAT
11S-Globulin CACCAAACCCGATCTTCAGT CGGAACAGCTTCTCCATCTC
19kD-Globulin GCCAGTAATTGCAGGGGATA AGGTCACCACCAACGTAAGC
Prolamin TCTCCAACCAACAATAGCAATG TGCGTAGCTATCTGTGCCCGTC
10kD-Prolamin TGCAGTATTTCCCACCAACA ACATGAACATGGCTGTGGAG
13kD-Prolamin CACAGCGCAGTTTGATGTTT GCTTGCCGCAATGCTATACT
17kD-Prolamin TTTGATGCTTGCACCTATGG GCAGCTGCTCAGTTTTAGCC
RA16 AGGTAGTGATCTCGGCGTTG CCGATTCCTGGCTGACATAG
RA17 TTCTCGGTATTGCTCCTCGT CTTATTCCTGGCCGACATTG

Table 2

Markers for mapping of EED1"

引物名
Primers ID		 正向引物序列
Forward sequences (5'-3')		 反向引物序列
Reverse sequences (5'-3')
OS907 CTTCTAACTTTTCCATCACATCG CCTCCAACTACCAGCCATAA
InDel950
InDel976		 ACTTTCTAACTTCCTAATAAG
GTTAAATCTTCTCTCAATCT		 GTTCTGGAATGAAATATTAGA
TCTCTCCGGTCTCTCCCTGC
RM107 AGATCGAAGCATCGCGCCCGAG ACTGCGTCCTCTGGGTTCCCGG
RM201 GTACTCTCGCCGTTCACAACTCC TTAGTGACCGGGATGACACAGC
RM245 ATGCCGCCAGTGAATAGC CTGAGAATCCAATTATCTGGGG
InDel908 TGGGAGAACTAAGGAGCAA CTGTTATCTGGCAATCTG

Fig. 1

Phenotypic analyses of mature caryopses of ZH11 and eed1 A: appearance comparison of the whole and mature caryopses between ZH11 and eed1, bar: 1 mm. B: cross-sections of ZH11 and eed1 mature caryopses, bar: 1 mm. C: comparison of mature caryopses size between ZH11 and eed1, n = 20. D: 1000-grain dry weight of ZH11 and eed1 mature grains, n = 3. Values are means ± SDs by Student’s t-test. **: P < 0.01. E-F: the scanning electron microscopic observation of mature caryopses of ZH11 (E) and eed1 (F), bar: 5 μm."

Fig. 2

Germination rate and cell viability of eed1 A: phenotypes of WT and eed1 mature caryopses at 7 days after imbibition, bar: 3 cm; B: germination rate of WT and eed1 mature caryopses at 7 days after imbibition; C-E: TTC staining in longitudinally sectioned mature caryopses of WT (C) and eed1 (D, E), bar: 1 mm; values are means ± SDs by Student’s t-test. **: P < 0.01."

Fig. 3

Caryopses phenotype analyses from regenerated plantlets of ZH11 and eed1 A-B: the eed1 callus can differentiate into adventitious buds and roots on RE2 medium (A), and eventually grow into plantlets (B), bar: 2 cm; C-D: changes in the fresh (C) and dry (D) weight of ZH11 and eed1 caryopses of different stages; E: phenotypes of fresh caryopses of ZH11 and eed1 at 0-30 days after pollination, bar: 1 mm; F: phenotypes of dry caryopses of ZH11 and eed1 at 24, 27, and 30 DAP (days after pollination), bar: 1 mm; values are means ± SDs by Student’s t-test. *: P < 0.05; **: P < 0.01."

Fig. 4

Starch contents and storage protein accumulations in eedl caryopses A-B: the determination of the contents of total starch (A), amylose (B) in ZH11, WT, and eed1 mature caryopses, n = 3. Values are means ± SDs by Student’s t-test; **: P < 0.01; C: SDS-PAGE profiles of total protein in ZH11, WT, and eed1 mature caryopses; αGT: glutelin acidic subunits; αGlb: α-globulin; βGT: glutelin basic subunits; Pro or Alb: prolamins or albumins."

Fig. 5

Relative expression profile of starch and storage protein biosynthesis genes in eed1 A: relative expression levels of genes involved in starch biosynthesis in endosperm of ZH11 and eed1; B: relative expression levels of genes involved in storage protein biosynthesis in endosperm of ZH11 and eed1."

Table 3

Genetic analysis of EED1 segregation using three heterozygous EED1/eed1 plants"

表型
Phenotype		 杂合植株-1
Heterozygous plant-1		 杂合植株-2
Heterozygous plant-2		 杂合植株-3
Heterozygous plant-3
正常籽粒 Normal grains 157 152 133
胚胎且胚乳缺陷籽粒 eed1 grains 47 50 45
χ2(3:1) 0.418 < 3.84 (P > 0.05) 0.006 < 3.84 (P > 0.05) 0.007 < 3.84 (P > 0.05)

Fig. 6

Genetic mapping of EED1 A: EED1 was linked with makers OS907 and InDel908 on the long arm of chromosome 9; B: EED1 was mapped to a 672 kb region based on 61 segregated plants. The region contained 114 ORFs."

[1] Nallamilli B R R, Zhang J, Mujahid H, Malone B M, Bridges S M, Peng Z H. Polycomb group gene OsFIE2 regulates rice(Oryza sativa) seed development and grain filling via a mechanism distinct from Arabidopsis. PLoS Genet, 2013,9:e1003322.
[2] Wu X B, Liu J X, Li D Q, Liu C M. Rice caryopsis development: I. Dynamic changes in different cell layers. J Integr Plant Biol, 2016,58:772-785.
[3] Itoh J I, Nonomura K I, Ikeda K, Yamaki S, Inukai Y, Yamagishi H, Kitano H, Nagato Y. Rice plant development: from zygote to spikelet. Plant Cell Physiol, 2005,46:23-47.
[4] Mizutani M, Naganuma T, Tsutsumi K I, Saitoh Y. The syncytium-specific expression of the Orysa; KRP3 CDK inhibitor: implication of its involvement in the cell cycle control in the rice (Oryza sativa L.) syncytial endosperm. J Exp Bot, 2010,61:791-798.
[5] 刘春明, 程佑发, 刘永秀, 孙蒙祥, 薛红卫. 植物种子发育的分子机理. 中国基础科学, 2016,18:1-13.
Liu C M, Cheng Y F, Liu Y X, Sun M X, Xue H W. Molecular mechanisms of seed development in plants. China Basic Sci, 2016,18:1-13 (in Chinese).
[6] 黄小荣. OsGCD1在雄配子体形成, 胚胎模式建成与胚乳发育中的功能研究. 武汉大学博士学位论文, 湖北武汉, 2017.
Huang X R. Functional Analysis of OsGCD1 in Male Gametophyte Formation, Embryo Pattern Formation and Endosperm Development of Rice (Oryza sativa). PhD Dissertation of Wuhan University, Wuhan, Hubei, China, 2017 (in Chinese with English abstract).
[7] Noriko K, Asuka N, Naoki S, Eriko T, Yasuo N, Hidemi K, Makoto M. Rice globular embryo 4 (gle4) mutant is defective in radial pattern formation during embryogenesis. Plant Cell Physiol, 2003,44:875-883.
[8] Itoh J I, Sato Y, Sato Y, Hibara K I, Shimizu-Sato S, Kobayashi H, Takehisa H, Sanguinet K A, Namiki N, Nagamura Y. Genome-wide analysis of spatiotemporal gene expression patterns during early embryogenesis in rice. Development, 2016,143:1217-1227.
[9] Huang X R, Peng X B, Sun M X. OsGCD1 is essential for rice fertility and required for embryo dorsal-ventral pattern formation and endosperm development. New Phytol, 2017,215:1039-1058.
[10] Lopes M A, Larkins B A. Endosperm origin, development, and function. Plant Cell, 1993,5:1383-1399.
[11] Liu J X, Wu X B, Yao X F, Yu R, Larkin P J, Liu C M. Mutations in the DNA demethylase OsROS1 result in a thickened aleurone and improved nutritional value in rice grains. Proc Natl Acad Sci USA, 2018,115:11327-11332.
[12] Wu X B, Liu J X, Li D Q, Liu C M. Rice caryopsis development: II. Dynamic changes in the endosperm. J Integr Plant Biol, 2016,58:786-798.
[13] Hara T, Katoh H, Ogawa D, Kagaya Y, Sato Y, Kitano H, Nagato Y, Ishikawa R, Ono A, Kinoshita T, Takeda S, Hattori T. Rice SNF2 family helicase ENL1 is essential for syncytial endosperm development. Plant J, 2015,81:1-12.
[14] 韩小花. 水稻籽粒中控制淀粉合成关键基因OsPDIL1-1的图位克隆及功能分析. 南京农业大学博士学位论文, 江苏南京, 2011.
Han X H. Map-based Cloning and Functional Analysis of a Key Gene OsPDIL1-1 Involved in the Starch Biosynthesis in Rice. PhD Dissertation of Nanjing Agricultural University, Nanjing, Jiangsu, China, 2011 (in Chinese with English abstract).
[15] Wei X J, Jiao G A, Lin H Y, Sheng Z H, Shao G N, Xie L H, Tang S Q, Xu Q G, Hu P S. GRAIN INCOMPLETE FILLING 2 regulates grain filling and starch synthesis during rice caryopsis development. J Integr Plant Biol, 2017,59:134-153.
[16] Ryoo N, Yu C, Park C S, Baik M Y, Park I M, Cho M H, Bhoo S H, An G, Hahn T R, Jeon J S. Knockout of a starch synthase gene OsSSIIIa/Flo5 causes white-core floury endosperm in rice( Oryza sativa L.). Plant Cell Rep, 2007,26:1083-1095.
[17] Wang W, Wei X, Jiao G, Chen W, Wu Y, Sheng Z, Hu S, Xie L, Wang J, Tang S, Hu P. GBSS-BINDING PROTEIN, encoding a CBM48 domain containing protein, affects rice quality and yield. J Integr Plant Biol, 2019,62:948-966.
[18] Wang J C, Xu H, Zhu Y, Liu Q Q, Cai X L. OsbZIP58, a basic leucine zipper transcription factor, regulates starch biosynthesis in rice endosperm. J Exp Bot, 2013,64:3453-3466.
[19] 刘巧泉, 周丽慧, 王红梅, 顾铭洪. 水稻种子贮藏蛋白合成的分子生物学研究进展. 分子植物育种, 2008,6:1-15.
Liu Q Q, Zhou L H, Wang H M, Gu M H. Advances on biosynthesis of rice seed storage proteins in molecular biology. Mol Plant Breed, 2008,6:1-15 (in Chinese with English abstract).
[20] Kawakatsu T, Yamamoto M P, Touno S M, Yasuda H, Takaiwa F. Compensation and interaction between RISBZ1 and RPBF during grain filling in rice. Plant J, 2009,59:908-920.
[21] She K C, Kusano H, Koizumi K, Yamakawa H, Hakata M, Imamura T, Fukuda M, Naito N, Tsurumaki Y, Yaeshima M, Tsuge T, Matsumoto K I, Kudoh M, Itoh E, Kikuchi S, Kishimoto N, Yazaki J, Ando T, Yano M, Aoyama T, Sasaki T, Satoh H, Shimada H. A novel factor FLOURY ENDOSPERM 2 is involved in regulation of rice grain size and starch quality. Plant Cell, 2010,22:3280-3294.
[22] Xing Y Z, Zhang Q. Genetic and molecular bases of rice yield. Annu Rev Plant Biol, 2010,61:421-442.
[23] An L, Tao Y, Chen H, He M J, Xiao F, Li G H, Ding Y F, Liu Z H. Embryo-endosperm interaction and its agronomic relevance to rice quality. Front Plant Sci, 2020,11:587641.
[24] Lafon-Placette C, Köhler C. Embryo and endosperm, partners in seed development. Curr Opin Plant Biol, 2014,17:64-69.
[25] Hehenberger E, Kradolfer D, Köhler C. Endosperm cellularization defines an important developmental transition for embryo development. Development, 2012,139:2031-2039.
[26] Pignocchi C, Minns G E, Nesi N, Koumproglou R, Kitsios G, Benning C, Lloyd C W, Doonan J H, Hills M J. ENDOSPERM DEFECTIVE 1 is a novel microtubule-associated protein essential for seed development in Arabidopsis. Plant Cell, 2009,21:90-105.
[27] Nagasawa N, Hibara K I, Heppard E P, Vander Velden K A, Luck S, Beatty M, Nagato Y, Sakai H. GIANT EMBRYO encodes CYP78A13, required for proper size balance between embryo and endosperm in rice. Plant J, 2013,75:592-605.
[28] Yang W B, Gao M J, Yin X, Liu J Y, Xu Y H, Zeng L J, Li Q, Zhang S B, Wang J M, Zhang X M, He Z H. Control of rice embryo development, shoot apical meristem maintenance, and grain yield by a novel cytochrome P450. Mol Plant, 2013,6:1945-1960.
[29] Xiong H X, Wang W, Sun M X. Endosperm development is an autonomously programmed process independent of embryogenesis. Plant Cell, 2021, doi: https://doi.org/10.1093/plcell/koab007.
[30] Zhang L, Qi Y Z, Wu M M, Zhao L, Zhao Z C, Lei C L, Hao Y Y, Yu X W, Sun Y L, Zhang X, Guo X P, Ren Y L, Wan J M. Mitochondrion-targeted PENTATRICOPEPTIDE REPEAT 5 is required for cis-splicing of nad4 intron 3 and endosperm development in rice. Crop J, 2020,9:282-296.
[31] Hiei Y, Komari T, Kubo T. Transformation of rice mediated by Agrobacterium tumefaciens. Plant Mol Biol, 1997,35:205-218.
[32] Hiei Y, Ohta S, Komari T, Kumashiro T. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J, 1994,6:271-282.
[33] Ren Y L, Wang Y H, Pan T, Wang Y L, Wang Y F, Gan L, Wei Z Y, Wang F, Wu M M, Jing R N, Wang J C, Wan G X, Bao X H, Zhang B L, Zhang P C, Zhang Y, Ji Y, Lei C L, Zhang X, Cheng Z J, Lin Q B, Zhu S S, Zhao Z C, Wang J, Wu C Y, Qiu L J, Wang H Y, Wan J M. GPA5 encodes a Rab5a effector required for post-golgi trafficking of rice storage proteins. Plant Cell, 2020,32:758-777.
[34] Zhao J, Qin J J, Song Q, Sun C Q, Liu F X. Combining QTL mapping and expression profile analysis to identify candidate genes of cold tolerance from Dongxiang common wild rice (Oryza rufipogon Griff.). J Integr Agric, 2016,15:1933-1943.
[35] Ohdan T, Francisco P B J, Sawada T, Hirose T, Terao T, Satoh H, Nakamura Y. Expression profiling of genes involved in starch synthesis in sink and source organs of rice. J Exp Bot, 2005,56:3229-3244.
[36] 梁子英, 刘芳. 实时荧光定量PCR技术及其应用研究进展. 现代农业科技, 2020, (6):1-3.
Lang Z Y, Liu F. Research progress on real-time quantitative PCR technology and its application. Mod Agric Sci Technol, 2020, (6):1-3 (in Chinese with English abstract).
[37] 李景芳, 田云录, 刘喜, 刘世家, 陈亮明, 江玲, 张文伟, 徐大勇, 王益华, 万建民. 鸟苷酸激酶OsGK1对水稻种子发育至关重要. 中国水稻科学, 2018,32:415-426.
Li J F, Tian Y L, Liu X, Liu S J, Chen M L, Jiang L, Zhang W W, Xu D Y, Wang Y H, Wan J M. The guanylate kinase OsGK1 is essential for seed development in rice. Chin J Rice Sci, 2018,32:415-426 (in Chinese with English abstract).
[38] 杜溢墨, 潘天, 田云录, 刘世家, 刘喜, 江玲, 张文伟, 王益华, 万建民. 水稻粉质皱缩胚乳突变体fse4的表型分析与基因克隆. 中国水稻科学, 2019,33:499-512.
Du Y M, Pan T, Tian Y L, Liu S J, Liu X, Jiang L, Zhang W W, Wang Y H, Wan J M. Phenotypic analysis and gene cloning of rice floury endosperm mutant fse4. Chin J Rice Sci, 2019,33:499-512 (in Chinese with English abstract).
[39] Hu T T, Tian Y L, Zhu J P, Wang Y L, Jing R N, Lei J, Sun Y L, Yu Y F, Li J F, Chen X L, Zhu X, Hao Y Y, Liu L L, Wang Y H, Wan J M. OsNDUFA9 encoding a mitochondrial complex I subunit is essential for embryo development and starch synthesis in rice. Plant Cell Rep, 2018,37:1667-1679.
[40] Wu M W, Zhao H, Zhang J D, Guo L, Liu C M. RADICLELESS 1 (RL1)-mediated nad4 intron 1 splicing is crucial for embryo and endosperm development in rice(Oryza sativa L.). Biochem Biophys Res Commun, 2020,523:220-225.
[41] 金田蕴, 李辉, 郭涛, 刘晓璐, 苏宁, 吴赴清, 万建民. 水稻稳定高垩白率突变体的获得与理化特性分析. 作物学报, 2010,36:121-132.
Jin T Y, Li H, Guo T, Liu X L, Su N, Wu F Q, Wan J M. Analysis of physiological and biochemical characteristics of six mutants with stable high percentage of chalkiness in rice grains. Acta Agron Sin, 2010,36:121-132 (in Chinese with English abstract).
[42] Teng X, Zhong M S, Zhu X P, Wang C M, Ren Y L, Wang Y L, Zhang H, Jiang L, Wang D, Hao Y Y, Wu M M, Zhu J P, Zhang X, Guo X P, Wang Y H, Wan J M. FLOURY ENDOSPERM 16 encoding a NAD-dependent cytosolic malate dehydrogenase plays an important role in starch synthesis and seed development in rice. Plant Biotechnol J, 2019,17:1914-1927.
[43] You X M, Zhang W W, Hu J L, Jing R N, Cai Y, Feng Z M, Kong F, Zhang J, Yan H G, Chen W W, Chen X G, Ma J, Tang X J, Wang P, Zhu S S, Liu L L, Jiang L, Wan J M. FLOURY ENDOSPERM 15 encodes a glyoxalase I involved in compound granule formation and starch synthesis in rice endosperm. Plant Cell Rep, 2019,38:345-359.
[44] Zhong M S, Liu X, Liu F, Ren Y L, Wang Y L, Zhu J P, Teng X, Duan E, Wang F, Zhang H, Wu M M, Hao Y Y, Zhu X P, Jing R N, Guo X P, Jiang L, Wang Y H, Wan J M. FLOURY ENDOSPERM 12 encoding Alanine Aminotransferase 1 regulates carbon and nitrogen metabolism in rice. J Plant Biol, 2019,62:61-73.
[45] Wang H H, Huang Y C, Xiao Q, Huang X, Li C S, Gao X Y, Wang Q, Xiang X L, Zhu Y D, Wang J C, Wang W Q, Larkins B A, Wu Y R. Carotenoids modulate kernel texture in maize by influencing amyloplast envelope integrity. Nat Commun, 2020,11:5346.
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