马铃薯苗期耐碱性鉴定方法及86份种质资源耐碱性综合评价

doi:10.3724/SP.J.1006.2023.24278

Abstract

Abstract:

China is facing a serious land salinization problem. The area of saline-alkaline land reaches 100 million hectares, of which about 33 million hectares of saline-alkaline land could be used. Previous researches mostly concentrate on saline tolerance in potato, only a few research focuses on alkaline tolerance. In this study, the morphological traits such as plant height, shoot fresh weight, root fresh weight, and rooting rate of five different varieties of potato tissue culture seedlings under different concentrations of NaHCO₃ simulated alkaline stress were measured, the corresponding coefficient of variation values were also calculated. Combined with coefficient of variation and the effective growth rate, the suitable simulated alkaline stress for alkaline tolerance assessment of potato tissue culture seedling was 5 mmol L^-1 NaHCO₃. Eighty-six kinds of tetraploid potato germplasms were treated with 5 mmol L^-1 NaHCO₃, alkaline tolerance coefficient of phenotypes including plant height, shoot fresh weight, root fresh weight, and rooting rate were measured. After principal component analysis and conversion by membership function, comprehensive assessment of alkaline tolerance (defined as A-value) was calculated. The A-values of Zhongshu 3 and Beifang 008 were the lowest and highest, respectively. Cluster analysis of comprehensive assessment of alkaline tolerance indicated that 86 kinds of tetraploid potato germplasms could be classified into 5 groups. Among them, the potato varieties (lines) with strong alkaline tolerance were Beifang 008, SDP632, SDP562, SDP274, SDP869, and SDP750. The regression equation between A-values and alkaline tolerance coefficients of phenotypes was established by stepwise regression analysis, A = 0.214X₁ + 0.341X₂ + 0.398X₃ - 0.177X₄ - 0.026. The four independent variables were the alkaline-tolerance coefficient of plant height, fresh weight of stem and leaf, fresh weight of root, and rooting rate. The comprehensive assessment system of alkaline tolerance of potato tissue culture seedlings established in this study could be helpful for the preliminary assessment of potato alkaline tolerance. The identified alkaline stress sensitive and tolerant gremplasms could be further used for the study of molecular regulation mechanism of alkaline stress tolerance and alkaline resistance breeding.

Key words: potato, seedling stage, alkaline stress tolerance, the comprehensive assessment

ZHAO Peng, CHEN Guang-Xia, ZHANG Yan-Ping, YANG Xiao-Hui, LIU Fang, DONG Dao-Feng. Alkaline tolerance identification method of potato seedlings and comprehensive assessment of alkaline tolerance of 86 kinds of potato germplasms[J].Acta Agronomica Sinica, 2023, 49(11): 2923-2934.

Figures/Tables 9

Table 1

Potato varieties and germplasms for assessment of alkaline stress tolerance"

名称 Name	类别 Category	名称 Name	类别 Category	名称 Name	类别 Category	名称 Name	类别 Category	名称 Name	类别 Category
大西洋Atlantic	栽培品种 Variety	SDP287	种质资源 Germplasm	SDP373	种质资源 Germplasm	SDP472	种质资源 Germplasm	SDP590	种质资源 Germplasm
宾杰 Bintje	栽培品种 Variety	SDP288	种质资源 Germplasm	SDP374	种质资源 Germplasm	SDP474	种质资源 Germplasm	SDP606	种质资源 Germplasm
底西瑞Desiree	栽培品种 Variety	SDP289	种质资源 Germplasm	SDP386	种质资源 Germplasm	SDP491	种质资源 Germplasm	SDP610	种质资源 Germplasm
费乌瑞它Favorita	栽培品种 Variety	SDP290	种质资源 Germplasm	SDP389	种质资源 Germplasm	SDP493	种质资源 Germplasm	SDP614	种质资源 Germplasm
米拉 Mira	栽培品种 Variety	SDP291	种质资源 Germplasm	SDP397	种质资源 Germplasm	SDP506	种质资源 Germplasm	SDP632	种质资源 Germplasm
夏波蒂Shepody	栽培品种 Variety	SDP292	种质资源 Germplasm	SDP398	种质资源 Germplasm	SDP527	种质资源 Germplasm	SDP671	种质资源 Germplasm
Sierra	栽培品种 Variety	SDP293	种质资源 Germplasm	SDP402	种质资源 Germplasm	SDP528	种质资源 Germplasm	SDP706	种质资源 Germplasm
北方001 Beifang 001	栽培品种 Variety	SDP297	种质资源 Germplasm	SDP404	种质资源 Germplasm	SDP531	种质资源 Germplasm	SDP707	种质资源 Germplasm
北方003 Beifang 003	栽培品种 Variety	SDP331	种质资源 Germplasm	SDP408	种质资源 Germplasm	SDP537	种质资源 Germplasm	SDP736	种质资源 Germplasm
北方007 Beifang 007	栽培品种 Variety	SDP334	种质资源 Germplasm	SDP414	种质资源 Germplasm	SDP539	种质资源 Germplasm	SDP744	种质资源 Germplasm
北方008 Beifang 008	栽培品种 Variety	SDP347	种质资源 Germplasm	SDP416	种质资源 Germplasm	SDP553	种质资源 Germplasm	SDP750	种质资源 Germplasm
黑金刚 Heijingang	栽培品种 Variety	SDP348	种质资源 Germplasm	SDP425	种质资源 Germplasm	SDP554	种质资源 Germplasm	SDP831	种质资源 Germplasm
陇薯9号 Longshu 9	栽培品种 Variety	SDP350	种质资源 Germplasm	SDP428	种质资源 Germplasm	SDP560	种质资源 Germplasm	SDP869	种质资源 Germplasm
闽薯1号 Minshu 1	栽培品种 Variety	SDP351	种质资源 Germplasm	SDP429	种质资源 Germplasm	SDP562	种质资源 Germplasm	SDP322Y	种质资源 Germplasm
青薯9号 Qingshu 9	栽培品种 Variety	SDP352	种质资源 Germplasm	SDP447	种质资源 Germplasm	SDP572	种质资源 Germplasm
希森3号 Xisen 3	栽培品种 Variety	SDP353	种质资源 Germplasm	SDP449	种质资源 Germplasm	SDP578	种质资源 Germplasm
中薯3号 Zhongshu 3	栽培品种 Variety	SDP365	种质资源 Germplasm	SDP458	种质资源 Germplasm	SDP583	种质资源 Germplasm
SDP274	种质资源 Germplasm	SDP371	种质资源 Germplasm	SDP461	种质资源 Germplasm	SDP584	种质资源 Germplasm

Table 1

Table 2

Phenotypes of potato tissue-culture seedlings under different concentrations of NaHCO3 stresses"

性状 Trait	品种/指标Variety/indicator	NaHCO₃浓度 NaHCO₃ concentration
性状 Trait	品种/指标Variety/indicator	0 mmol L^-1	2.5 mmol L^-1	5.0 mmol L^-1	7.5 mmol L^-1	10.0 mmol L^-1
株高 Plant height (mm)	宾杰Bintje	71.7±5.2 a	68.5±4.5 a	33.8±5.1 b	16.8±7.3 c	3.3±0.5 d
	SDP289	87.1±3.1 a	85.1±3.3 a	54.6±6.8 b	19.4±5.2 c	3.1±0.4 d
	SDP292	75.6±5.4 a	76.6±1.2 a	33.0±1.5 b	9.8±1.5 c	13.3±3.4 c
	SDP293	82.9±3.6 a	90.3±7.1 a	74.9±9.4 a	45.1±11.5 b	6.2±0.3 c
	SDP297	76.9±7.1 a	73.4±7.7 a	69.1±5.3 a	27.0±3.7 b	10.3±0.8 c
	平均值 Mean	78.9	78.8	53.1	23.6	7.2
	标准差 SD	5.5	7.9	17.4	12.1	4.0
	变异系数 CV	6.9%	10.0%	32.8%	51.2%	55.0%
茎叶鲜重 Shoot fresh weight (mg)	宾杰Bintje	90.3±7.9 a	53.5±10.7 b	46.8±12.3 b	9.5±3.4 c	3.4±0.3 c
	SDP289	250.7±56.7 a	145.0±2.7 b	161.3±5.4 b	51.3±11.0 c	9.7±3.5 c
	SDP292	200.0±34.3 a	122.9±7.7 b	82.3±19.8 bc	48.9±11.1 cd	10.9±3.1 d
	SDP293	168.8±14.5 ab	172.0±19.0 a	133.9±25.1 b	56.3±7.4 c	9.4±2.3 d
	SDP297	234.5±25.2 a	132.9±5.4 c	181.6±19.5 b	42.8±5.5 d	26.8±7.9 d
	平均值 Mean	188.9	125.3	121.2	41.8	12.0
	标准差 SD	56.8	39.5	49.9	16.7	7.8
	变异系数 CV	30.1%	31.5%	41.2%	40.0%	65.0%
根鲜重 Root fresh weight (mg)	宾杰 Bintje	39.1±0.3 a	18.3±3.1 b	7.8±0.9 c	9.0±1.1 c	0.0±0.0 d
	SDP289	102.4±11.9 a	32.3±8.0 b	39.6±2.6 b	6.3±2.8 c	0.0±0.0 c
	SDP292	86.3±3.5 a	31.3±1.9 b	19.2±6.1 c	0.0±0.0 d	0.0±0.0 d
	SDP293	70.0±5.8 a	49.6±1.9 b	59.0±8.9 ab	7.4±2.0 c	0.0±0.0 c
	SDP297	61.1±6.8 a	30.3±3.5 b	33.7±4.6 b	0.7±0.2 c	0.0±0.0 c
	平均值 Mean	71.8	32.4	31.9	4.7	0.0
	标准差 SD	21.6	10.0	17.5	3.7	0.0
	变异系数 CV	30.1%	30.9%	55.0%	78.2%	0.0%
生根率 Rooting rate (%)	宾杰 Bintje	100.0±0.0 a	100.0±0.0 a	66.7±4.7 b	23.3±4.7 c	0.0±0.0 d
	SDP289	100.0±0.0 a	93.3±9.4 a	80.0±8.2 b	33.3±4.7 c	0.0±0.0 d
	SDP292	100.0±0.0 a	86.7±4.7 b	56.7±4.7c	0.0±0.0 d	0.0±0.0 d
	SDP293	100.0±0.0 a	100.0±0.0 a	86.7±9.4 b	40.0±8.2 c	0.0±0.0 d
	SDP297	100.0±0.0 a	100.0±0.0 a	76.7±9.4 b	36.7±9.4 c	0.0±0.0 d
	平均值 Mean	100.0	96.0	73.3	26.7	0.0
	标准差 SD	0.0	5.3	10.5	14.5	0.0
	变异系数 CV	0	5.6%	14.4%	54.2%	0
有效生长率Effective growth rate (%)	宾杰Bintje	100.0±0.0 a	100.0±0.0 a	80.0±8.2 b	53.3±4.7 c	0.0±0.0 d
	SDP289	100.0±0.0 a	100.0±0.0 a	93.3±9.4 a	50.0±8.2 b	6.7±4.7 c
	SDP292	100.0±0.0 a	100.0±0.0 a	76.7±4.7 b	36.7±4.7 c	16.7±4.7 d
	SDP293	100.0±0.0 a	100.0±0.0 a	100.0±0.0 a	86.7±9.4 a	53.3±9.4 b
	SDP297	100.0±0.0 a	100.0±0.0 a	100.0±0.0 a	63.3±9.4 b	30.0±8.2 c
	平均值 Mean	100.0	100.0	90.0	58.0	21.3

Table 2

Table 3

Fig. 1

Table 4

Alkaline tolerance coefficients of phenotypes of 86 potato varieties (lines)"

品种(系) Variety (line)	株高 Plant height	茎叶鲜重 Shoot fresh weight	根鲜重 Root fresh weight	生根率 Rooting rate	品种(系) Variety (line)	株高 Plant height	茎叶鲜重 Shoot fresh weight	根鲜重 Root fresh weight	生根率 Rooting rate
大西洋Atlantic	0.886	0.988	0.718	1.000	SDP404	0.821	0.627	0.537	1.000
宾杰Bintje	0.432	0.639	0.323	0.467	SDP408	0.838	1.021	0.777	1.000
底西瑞Desiree	0.587	0.614	0.312	0.500	SDP414	0.789	0.793	0.553	0.938
费乌瑞它Favorita	0.775	1.039	0.725	0.600	SDP416	0.884	0.812	0.869	1.000
米拉Mira	0.813	1.021	0.317	0.867	SDP425	1.008	0.949	0.402	0.875
夏波蒂Shepody	0.725	0.797	0.709	0.500	SDP428	0.530	0.618	0.033	0.938
Sierra	0.951	0.842	0.823	1.000	SDP429	0.763	0.707	0.560	1.000
北方001 Beifang 001	0.529	0.620	0.505	1.000	SDP447	1.066	1.164	0.564	1.000
北方003 Beifang 003	0.971	0.955	0.762	1.000	SDP449	0.762	0.707	0.297	0.813
北方007 Beifang 007	0.828	0.886	0.874	0.600	SDP458	0.973	0.808	0.807	0.938
北方008 Beifang 008	0.979	1.417	0.601	0.300	SDP461	0.575	0.914	0.288	0.875
黑金刚Heijingang	0.845	1.023	0.592	1.000	SDP472	0.727	0.928	0.307	0.750
陇薯9号Longshu 9	0.341	0.452	0.329	0.563	SDP474	0.426	0.478	0.786	0.545
闽薯1号Minshu 1	0.542	0.659	0.241	0.824	SDP491	0.358	0.594	0.074	0.333
青薯9号Qingshu 9	0.760	0.717	0.270	0.727	SDP493	0.710	1.009	0.950	0.985
希森3号Xisen 3	0.432	0.570	0.495	0.438	SDP506	0.916	1.082	0.768	1.000
中薯3号Zhongshu 3	0.076	0.091	0.128	0.250	SDP527	0.564	0.912	0.462	0.647
SDP274	1.035	1.229	0.827	1.000	SDP528	0.593	0.948	0.700	0.750
SDP287	0.585	0.635	0.323	0.813	SDP531	0.252	0.524	0.264	0.375
SDP288	0.284	0.420	0.219	0.375	SDP537	0.676	0.543	0.484	0.688
SDP289	0.197	0.331	0.241	0.375	SDP539	0.081	0.182	0.059	0.083
SDP290	0.563	0.570	0.452	0.500	SDP553	0.802	0.740	0.400	1.000
SDP291	0.240	0.210	0.116	0.438	SDP554	0.534	0.534	0.333	0.750
SDP292	0.135	0.169	0.102	0.188	SDP560	0.831	0.931	0.644	1.000
SDP293	0.857	0.759	0.302	0.941	SDP562	1.199	1.478	0.610	1.000
SDP297	0.752	0.676	0.514	0.938	SDP572	0.577	0.631	0.460	1.000
SDP331	0.707	0.883	0.415	0.667	SDP578	0.908	0.968	0.542	0.910
SDP334	0.692	0.797	0.487	0.933	SDP583	0.752	0.781	0.601	0.938
SDP347	0.683	1.238	0.260	0.533	SDP584	0.910	0.881	0.669	1.000
SDP348	0.813	0.932	0.884	1.000	SDP590	0.794	0.999	0.745	0.667
SDP350	0.627	0.629	0.307	0.688	SDP606	0.243	0.236	0.234	0.551
SDP351	0.664	0.801	0.508	0.688	SDP610	0.657	0.549	0.260	1.000
SDP352	0.925	0.991	0.825	1.000	SDP614	0.493	0.868	0.298	0.938
SDP353	0.440	0.407	0.248	0.500	SDP632	1.122	1.275	0.812	0.889
SDP365	0.818	0.85	0.564	1.000	SDP671	0.683	0.674	0.418	0.882
SDP371	0.786	0.756	0.499	1.000	SDP706	0.616	0.716	0.403	0.407
SDP373	0.607	0.720	0.342	1.000	SDP707	0.613	0.562	0.568	0.750
SDP374	0.664	0.627	0.367	0.750	SDP736	0.554	0.777	0.344	0.926
SDP386	0.837	0.895	0.883	0.882	SDP744	0.289	0.321	0.245	0.500
SDP389	0.567	0.604	0.380	0.813	SDP750	1.024	1.140	0.868	0.969
SDP397	0.393	0.616	0.266	0.667	SDP831	0.887	0.922	1.043	1.000
SDP398	0.396	0.598	0.346	0.750	SDP869	0.837	1.061	0.951	0.727
SDP402	0.709	0.821	0.512	0.938	SDP322Y	0.651	1.109	1.005	1.000

Table 4

Table 5

Table 6

Table 7

Fig. 2

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指标 Indicator	株高 Plant height	茎叶鲜重 Shoot fresh weight	根鲜重 Root fresh weight
茎叶鲜重Shoot fresh weight	0.851^**
根鲜重Root fresh weight	0.687^**	0.644^**
生根率Rooting rate	0.706^**	0.537^**	0.507^**

综合指标 Comprehensive indicator	特征值 Eigen value	贡献率 Contribution ratio (%)	累计贡献率 Cumulative contribution ratio (%)	特征向量 Eigenvector
综合指标 Comprehensive indicator	特征值 Eigen value	贡献率 Contribution ratio (%)	累计贡献率 Cumulative contribution ratio (%)	株高 Plant height	茎叶鲜重 Shoot fresh weight	根鲜重 Root fresh weight	生根率 Rooting rate
PC1	2.980	74.501	74.501	0.246	0.492	0.611	-0.459
PC2	0.519	12.966	87.467	0.202	-0.170	-0.372	1.081

Alkaline tolerance identification method of potato seedlings and comprehensive assessment of alkaline tolerance of 86 kinds of potato germplasms

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品种(系) Variety (line)	A值 A-value	品种(系) Variety (line)	A值 A-value	品种(系) Variety (line)	A值 A-value	品种(系) Variety (line)	A值 A-value	品种(系) Variety (line)	A值 A-value
大西洋Atlantic	0.609	SDP287	0.300	SDP373	0.309	SDP472	0.435	SDP590	0.663
宾杰Bintje	0.330	SDP288	0.199	SDP374	0.343	SDP474	0.445	SDP606	0.102
底西瑞Desiree	0.345	SDP289	0.159	SDP386	0.654	SDP491	0.223	SDP610	0.228
费乌瑞它Favorita	0.676	SDP290	0.380	SDP389	0.309	SDP493	0.674	SDP614	0.328
米拉Mira	0.469	SDP291	0.066	SDP397	0.256	SDP506	0.668	SDP632	0.815
夏波蒂Shepody	0.595	SDP292	0.068	SDP398	0.268	SDP527	0.475	SDP671	0.360
Sierra	0.615	SDP293	0.370	SDP402	0.443	SDP528	0.570	SDP706	0.438
北方001 Beifang 001	0.323	SDP297	0.404	SDP404	0.400	SDP531	0.245	SDP707	0.390
北方003 Beifang 003	0.634	SDP331	0.473	SDP408	0.634	SDP537	0.375	SDP736	0.330
北方007 Beifang 007	0.695	SDP334	0.423	SDP414	0.467	SDP539	0.062	SDP744	0.154
北方008 Beifang 008	0.852	SDP347	0.551	SDP416	0.609	SDP553	0.380	SDP750	0.756
黑金刚Heijingang	0.562	SDP348	0.641	SDP425	0.518	SDP554	0.270	SDP831	0.716
陇薯9号Longshu 9	0.232	SDP350	0.323	SDP428	0.145	SDP560	0.549	SDP869	0.765
闽薯1号Minshu 1	0.265	SDP351	0.470	SDP429	0.424	SDP562	0.800	SDP322Y	0.714
青薯9号Qingshu 9	0.360	SDP352	0.661	SDP447	0.646	SDP572	0.319
希森3号Xisen 3	0.380	SDP353	0.217	SDP449	0.352	SDP578	0.553
中薯3号Zhongshu 3	0.028	SDP365	0.486	SDP458	0.613	SDP583	0.474
SDP274	0.767	SDP371	0.422	SDP461	0.368	SDP584	0.558

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