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Acta Agronomica Sinica ›› 2023, Vol. 49 ›› Issue (7): 1994-2001.doi: 10.3724/SP.J.1006.2023.24196

• RESEARCH NOTES • Previous Articles     Next Articles

Functional analysis of StZIP12 in regulating potato Zn uptake

SUO Hai-Cui(), LIU Ji-Tao, WANG Li, LI Cheng-Chen, SHAN Jian-Wei, LI Xiao-Bo*()   

  1. Crops Research Institute, Guangdong Academy of Agricultural Sciences / Guangdong Province Key Laboratory of Crop Genetic Improvement, Guangzhou 510640, Guangdong, China
  • Received:2022-08-26 Accepted:2022-11-28 Online:2023-07-12 Published:2022-12-12
  • Contact: *E-mail: Lixiaobo1981@163.com E-mail:suohaicui@163.com;Lixiaobo1981@163.com
  • Supported by:
    The National Natural Science Foundation of China’s Youth Fund(31701486)


Potato is the fourth largest food crop in the world. Two-thirds of the world’s population feed on potato, which is an important target crop for zinc biofortification strategy. It is of great importance to study zinc uptake in potato. Zinc transporters regulate zinc uptake in plants. In this study, we cloned potato zinc transporter StZIP12 on the basis of previous studies. Quantitative PCR results showed that StZIP12 was expressed in all potato tissues, and the relative expression level of StZIP12 in young leaves was significantly higher than that in other tissues. In addition, low zinc treatment could also induce StZIP12 expression. The heterologous yeast complementation test confirmed that StZIP12 could restore the zinc absorption function of ZHY3 (zrt1zrt2) yeast mutant. We obtained StZIP12 overexpressing transgenic plants in cultivar potato Eshu 3 by genetic transformation method. Under zinc deficiency treatment, the overexpression of StZIP12 increased plant height of transgenic plants, root length, and the total zinc content of transgenic seedlings, and the zinc content of tubers of pot-cultured transgenic plants. The zinc content of tubers of two lines was 22.00% and 32.95% higher than that of the control, respectively. These results prove that StZIP12 playes an important role in the absorption of zinc in potato, and provides a theoretical basis for the biofortification of potato zinc.

Key words: potato, zinc transporter, StZIP12, zinc absorption

Fig. 1

Cloning and transmembrane domain analysis of StZIP12 gene A: the isolation of StZIP12 gene. Lane 1, 2, and 3 are all potato StZIP12 genes. M2000 is DL 2000 DNA marker. B: the sequence analysis of StZIP12, gray letters in the background represent amino acid sequences. C: StZIP12 transmembrane domain analysis. The yellow area represents the transmembrane structure and the gray area represents the cell membrane."

Fig. 2

StZIP12 protein is subcellular localized on the cell membrane Bar: 50 μm."

Fig. 3

Relative expression pattern of StZIP12 genes Error bar represents standard error. Duncan’s analysis method is used for multiple comparisons. Different lowercase represents significant difference at P < 0.05."

Fig. 4

Functional complementation of StZIP12 to yeast zinc uptake deficient mutant VC: the empty vector controls; 10-1-10-4 represent the dilution concentration of bacterial solution, respectively."

Fig. 5

Detection of transgenic lines by qRT-PCR ES3: non transgenic control; OE-26, OE-27, OE-28, OE-31, OE-32, OE-47, and OE-67 are overexpressed StZIP12 lines."

Fig. 6

Phenotype and zinc content of potato transgenic tissue culture seedlings under zinc deficiency treatments A: plant phenotype analysis. Error bar represents standard error. Duncan’s analysis method is used for multiple comparisons. Different lowercase represents significant difference at P < 0.05. B: plant phenotype; C: the classification of root volume. CK: the normal zinc treatment;-Zn: zinc deficiency treatment."

Fig. 7

Phenotype, plant height, and zinc content of transgenic lines under zinc deficiency treatment CK: normal zinc treatment;-Zn: zinc deficiency treatment. Error bar represents standard error. Duncan’s analysis method is used for multiple comparisons. Different lowercase represents significant difference at P < 0.05."

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