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Acta Agronomica Sinica ›› 2023, Vol. 49 ›› Issue (4): 1102-1110.doi: 10.3724/SP.J.1006.2023.24078

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Potato tuber greening evaluation based on RGB color space

WANG Shuo(), BAO Tian-Yang, LIU Jian-Gang, DUAN Shao-Guang, JIAN Yin-Qiao, LI Guang-Cun, JIN Li-Ping, XU Jian-Fei*()   

  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture and Rural Affairs, Beijing 100081, China
  • Received:2022-04-02 Accepted:2022-09-05 Online:2023-04-12 Published:2022-09-15
  • Contact: *E-mail: xujianfei@caas.cn
  • Supported by:
    Agricultural Breeding Project of Ningxia Hui Autonomous Region, China(2019NYYZ01-1);China Agriculture Research System of MOF and MARA(CARS-09)

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Abstract:

Potato is one of the most important crops in the world. Tuber is the commercial product of potato, whereas tuber greening seriously affects product quality and results in a large amount of potatoes waste. It is very necessary to establish a convenient evaluation method of tuber greening for the identification of potato germplasm resources and the analysis of tuber quality. In this study, we systematically evaluated the greening trend and greening degree of 15 potato tetraploid and diploid varieties (lines) base on the extraction of tuber color space characteristics and RGB color space color difference. Zhongshu 2, Zhongshu 18, Zhongshu 19, Atlantic, Favorita, and Kondor appeared faster greening speed, but HS66 appeared slower greening speed under 25℃ and 125 μmol m-2 s-1. Color difference value of tubers of all potato varieties (lines) tended to the stability after 9 days lighting treatment. There was an obvious greening extent differences among potato varieties (lines). Among them, the afforestation degree of Favorita was the highest, whereas Zhongshu 28 was the lowest after 13 days lighting treatment. The method established in this study can accurately distinguish the greening speed and the extent of different varieties (lines), which provided a convenient and reliable technical reference for tuber greening evaluation.

Key words: potato, tuber, greening, RGB color features, color difference analysis

Table 1

Correlation analysis between color differences from two tubers"

品种(系)
Variety (line)		 光照处理1 d后色差
Color difference after 1
day of light treatment		 光照处理7 d后色差
Color difference after 7
days of light treatment		 光照处理13 d后色差
Color difference after 13
days of light treatment
块茎1 Tuber 1 块茎2 Tuber 2 块茎1 Tuber 1 块茎2 Tuber 2 块茎1 Tuber 1 块茎2 Tuber 2
中薯2号 Zhongshu 2 59.82 59.09 48.74 47.01 46.75 44.41
中薯3号 Zhongshu 3 69.51 75.51 57.48 63.48 55.60 60.84
中薯5号 Zhongshu 5 71.11 73.81 56.70 61.73 53.81 57.32
中薯18号 Zhongshu 18 71.43 67.22 52.82 53.24 52.10 51.30
中薯19号 Zhongshu 19 78.00 75.58 61.13 60.40 59.16 59.40
中薯21号 Zhongshu 21 69.25 77.15 52.98 59.93 50.80 56.40
中薯27号 Zhongshu 27 71.13 72.22 46.01 52.16 44.51 50.35
中薯28号 Zhongshu 28 61.70 61.84 55.09 55.03 54.02 52.40
Atlantic 63.95 63.01 55.42 54.81 55.35 54.18
CE125 77.98 82.72 58.12 57.86 55.93 55.68
Favorita 71.19 68.72 43.29 42.83 41.35 40.73
HS66 54.10 65.57 47.75 56.09 46.28 53.19
Kondor 57.84 54.39 43.37 43.27 42.01 41.32
RH 76.38 73.28 56.73 51.73 54.91 49.30
Shepody 68.80 77.71 56.71 58.78 59.53 58.43
相关系数
Correlation coefficient		 0.785** 0.802** 0.823**

Fig. 1

Tendency of color difference in different varieties (lines)"

Table 2

Color difference of tuber of potato varieties (lines) under different light treatments during different periods"

品种(系)
Variety (line)		 时间Time
0 d
0 DAL		 1 d后
1 DAL		 3 d后
3 DAL		 5 d后
5 DAL		 7 d后
7 DAL		 9 d后
9 DAL		 11 d后
10 DAL		 13 d后
13 DAL
中薯2号 Zhongshu 2 67.58 a 59.45 b 49.02 c 47.83 cd 47.88 cd 47.02 cd 45.98 d 45.58 d
中薯3号 Zhongshu 3 76.49 a 72.51 ab 64.56 bc 61.73 c 60.48 c 59.69 c 59.47 c 58.22 c
中薯5号 Zhongshu 5 73.48 a 72.46 a 65.90 b 60.26 bc 59.22 bc 56.57 c 56.25 c 55.56 c
中薯18号 Zhongshu 18 79.31 a 69.32 b 55.79 c 53.17 c 53.03 c 51.97 c 52.71 c 51.70 c
中薯19号 Zhongshu 19 85.00 a 76.79 b 64.97 c 61.59 d 60.76 de 60.35 de 60.18 de 59.28 e
中薯21号 Zhongshu 21 78.42 a 73.02 a 58.42 b 56.44 b 56.46 b 55.93 b 54.27 b 53.06 b
中薯27号 Zhongshu 27 74.82 a 71.68 a 57.45 b 49.80 bc 49.09 bc 47.16 c 46.88 c 47.43 c
中薯28号 Zhongshu 28 61.76 a 61.77 a 57.40 b 55.86 c 55.06 cd 53.94 de 53.97 de 53.21 e
Atlantic 71.77 a 63.48 b 56.46 c 54.54 c 55.11 c 54.53 c 54.36 c 54.76 c
CE125 84.68 a 80.35 a 65.76 b 58.82 c 57.99 c 56.99 c 56.00 c 55.81 c
Favorita 76.67 a 69.96 b 49.70 c 43.74 d 43.06 d 42.76 d 42.37 d 41.04 d
HS66 65.08 a 59.84 ab 53.52 ab 51.79 ab 51.92 ab 50.57 ab 48.67 b 49.73 ab
Kondor 59.32 a 56.11 b 47.59 c 44.26 d 43.32 d 42.21 d 42.06 d 41.67 d
RH 78.41 a 74.83 a 56.60 b 50.68 b 54.23 b 54.13 b 52.43 b 52.11 b
Shepody 78.36 a 73.25 a 62.70 b 59.53 b 57.74 b 60.00 b 58.72 b 58.98 b

Fig. 2

Difference of greening extent among different varieties (lines) Duncan’s test is used for data analysis and error bars in the figure indicate standard error. Different lowercase letters indicate significant differences at the 0.05 probability level."

Fig. 3

Tuber greening changes of potato varieties (lines) used in this study DAL: days after light treatment."

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