Fig. 1

Cloning and transmembrane domain analysis of StZIP12 gene A: the isolation of StZIP12 gene. Lane 1, 2, and 3 are all potato StZIP12 genes. M2000 is DL 2000 DNA marker. B: the sequence analysis of StZIP12, gray letters in the background represent amino acid sequences. C: StZIP12 transmembrane domain analysis. The yellow area represents the transmembrane structure and the gray area represents the cell membrane."

Fig. 2

StZIP12 protein is subcellular localized on the cell membrane Bar: 50 μm."

Fig. 3

Relative expression pattern of StZIP12 genes Error bar represents standard error. Duncan’s analysis method is used for multiple comparisons. Different lowercase represents significant difference at P < 0.05."

Fig. 4

Functional complementation of StZIP12 to yeast zinc uptake deficient mutant VC: the empty vector controls; 10-1-10-4 represent the dilution concentration of bacterial solution, respectively."

Fig. 5

Detection of transgenic lines by qRT-PCR ES3: non transgenic control; OE-26, OE-27, OE-28, OE-31, OE-32, OE-47, and OE-67 are overexpressed StZIP12 lines."

Fig. 6

Phenotype and zinc content of potato transgenic tissue culture seedlings under zinc deficiency treatments A: plant phenotype analysis. Error bar represents standard error. Duncan’s analysis method is used for multiple comparisons. Different lowercase represents significant difference at P < 0.05. B: plant phenotype; C: the classification of root volume. CK: the normal zinc treatment;-Zn: zinc deficiency treatment."

Fig. 7

Phenotype, plant height, and zinc content of transgenic lines under zinc deficiency treatment CK: normal zinc treatment;-Zn: zinc deficiency treatment. Error bar represents standard error. Duncan’s analysis method is used for multiple comparisons. Different lowercase represents significant difference at P < 0.05."