Fig. 1

Diagram of auxin responsible vector pc2300-DR5::GUS"

Table 1

Primers for identification of positive transgenic plants"

Table 2

Component PCR system"

Fig. 2

Identification of GUS positive plants by PCR and GUS staining A: PCR identification of transgenic rapeseed plants with kanamycin resistance. M: marker, and the target band size was 705 bp. 8 transgenic single plants were 1-8, 9 was positive controls, and 10 was negative controls. B: GUS staining identification of transgenic rape plants with kanamycin resistance."

Fig. 3

Transgenic DR5::GUS rapeseed seedlings stained for GUS activity at different development stages A, C, E-I: GUS staining identification of seedlings from 1 to 15 days after germination. B, D: GUS staining identification of root tips of seedlings 1 and 2 days after germination. DAG: days after germination. Bar in Figs. E-I: 1 cm."

Fig. 4

DR5::GUS and GH3.6 gene expression levels in transgenic rapeseed seedlings at different developmental stages Significance analysis was performed using the t-test (n ≥ 3). * and ** indicate significant difference at the 0.05 and 0.01 probability levels, respectively. DAG: days after germination."

Fig. 5

GUS staining of DR5::GUS transgenic rapeseed seedlings under different concentrations of NAA treatment Bar: 1 cm."

Fig. 6

DR5::GUS staining of GUS transgenic rape in bud"

Fig. 7

DR5::GUS staining of GUS transgenic rape in seed A-J: GUS staining identification of seed from 1 to 20 days after pollination. DAP: days after pollination. Bar: 500 μm."

Fig. 8

DR5::GUS staining of GUS transgenic rape in silique DAP: days afer pollination."